Using a modified Boyden chamber system, inflammatory exudates taken from the acute carrageenan pleural model and the chronic carrageenan air-pouch model were tested for their ability to promote leukocyte locomotion in vitro. Both exudates were able to induce polymorphonuclear leukocyte (PMN) and mononuclear leukocyte (MN) migration, suggesting that cell-specific chemoattractants are not responsible for the progression of the inflammatory process from the acute to the chronic phase. Checkerboard assays were used to establish whether the observed migration was in response to chemotactic stimulation and/or chemokinetic stimulation. Both acute and chronic exudates were able to induce MN chemotaxis and chemokinesis. Acute exudate was able to induce PMN chemotaxis and chemokinesis, but chronic exudate was only able to induce PMN chemokinesis. This may partly explain the predominance of MNs in chronic inflammation. However, our present in vitro results have failed to demonstrate that cell-specific chemoattractants are responsible for the in vivo observation that migration of polymorphonuclear leukocytes precedes the migration of mononuclear cells.
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Spector, W. G. 1969,Int. Rev. Exp. Pathol. The granulomatous exudate.8:1–55.
Wilkinson, P. C., andR. B. Allan. 1980. The locomotor behavior of human blood monocytes in chemotactic and chemokinetic environments and the role of substratum in monocyte locomotion.In Mononuclear Phagocytes, Part I. Martinus Nijoff. R. Van Furth, editor. The Hague, Boston/London.
Zigmond, S. H., andJ. G. Hirsch. 1973. Leukocyte locomotion and chemotaxis. Methods for evaluation and demonstration of cell chemotactic factors.J. Exp. Med. 137:387–397.
Velo, G. P., C. J. Dunn, J. P. Giroud, J. Timsit, andD. A. Willoughby. 1973. Distribution of prostaglandins in inflammatory exudate.J. Pathol. 3:149–158.
Wilkinson, P. C. 1974. Chemotaxis and Inflammation. Churchill-Livingstone, Edinburgh.
Capasso, F., C. J. Dunn, S. Yamamoto, D. A. Willoughby, andJ. P. Giroud. 1975. Further studies on carrageenan induced pleurisy in rats.J. Pathol. 116:117–124.
William, D. M., andN. W. Johnson. 1976. Alterations in peripheral blood leukocyte distribution in response to local inflammation stimuli in the rat.J. Pathol. 118:129–134.
Sultan, A.M., C. J. Dunn, andD. A. Willoughby. 1979. Leukocyte migration inhibition activity of nonimmune acute inflammatory pleural exudate.Inflammation 3(3):305–317.
Atherton, A., andG. V. R. Born. 1972. Quantitative investigations of adhesiveness of circulating polymorphonuclear leukocytes to blood vessel walls.J. Physiol. 222:447–474.
Gesner, B. M., andV. Ginsburg. 1964. Effect of glycosides on the fate of transfused lymphocytes.Proc. Natl. Acad. Sci. (U.S.A.) 52:750–755.
Sedgwick, A. D., J. C. W.Edwards, D. A.Willoughby. 1981.J. Path, (in press).
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Sedgwick, A.D., Edwards, J.C.W. & Willoughby, D.A. Studies on the ability of inflammatory exudate obtained from acute and chronic phases of the inflammatory process to promote leukocyte locomotion in vitro. Inflammation 6, 13–20 (1982). https://doi.org/10.1007/BF00910715
- Public Health
- Internal Medicine
- Mononuclear Cell
- Inflammatory Process
- Chronic Inflammation