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Anwendung der Affinitätschromatographie an NAD-Sepharose zum Nachweis und zur Reinigung vonmyo-Inosit-1-phosphat-Synthase in Erythrocyten von Hühnern und inLemna gibba

Studies on the biosynthesis of cyclitols, XXXII: Application of affinity chromatography on NAD-sepharose to the identification and purification of myo-inositol-1-phosphate synthase in chicken erythrocytes and in lemna gibba

Untersuchungen über die Biosynthese der Cyclite, 32. Mitt.

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Abstract

With the help of affinity chromatography on NAD-Sepharose it was possible to findmyo-inositol-1-phosphate synthase (EC 5.5.1.4) in blood of anaemic chicken and to obtain a relatively active preparation of the enzyme. In the blood of normal chicken, which had no reticulocytes, no activity of this enzyme could be detected. The same method was also successfully applied for the concentration of an enzyme of the same specificity fromLemna gibba (duckweed).

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31. Mitt.:W. Woloszczuk undO. Hoffmann-Ostenhof, Z. physiol. Chem. (im Druck).

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Schwarcz, R., Fried, W., Pittner, F. et al. Anwendung der Affinitätschromatographie an NAD-Sepharose zum Nachweis und zur Reinigung vonmyo-Inosit-1-phosphat-Synthase in Erythrocyten von Hühnern und inLemna gibba . Monatshefte für Chemie 105, 445–451 (1974). https://doi.org/10.1007/BF00907394

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