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Microcalorimetry as a diagnostic and analytical tool for the assessment of biodegradation of 2,4-D in a liquid medium and in soil

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The biodegradation of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) byPseudomonas cepacia was assessed by microcalorimetry in a liquid medium and in sterilized soil at 25°C under aerobic conditions. It was found that thermograms of the rate of heat evolved versus time (dQ/dt versust) can be used as a diagnostic tool to identify the timet 1 required for the primary biodegradation of 2,4-D and the timet f required for the completion of the biodegradation activity in a liquid medium as well as in soil. Microcalorimetry can also be used as an analytical tool to monitor the progress of 2,4-D consumption during the biodegradation process in a liquid medium and to measure the importance of the soil sorption/desorption of intermediate metabolites. A new concept called “bioeffort” was defined as the product of the biodegradation time (t) and the biomass concentration (X) at that time. This concept was used to predict either the biomass concentration required or the duration of the primary biodegradation of 2,4-D in soil from the data obtained from a liquid medium.

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  1. Baudu M, Le Cloirec P, Martin G (1993) First approach of desorption energies of water and organic molecules onto activated carbon by differential scanning calorimetry studies. Water Res 27:69–76

  2. Cooney CL, Wang DIC, Mateles RI (1968) Measurement of heat evolution and correlation with oxygen consumption during microbial growth. Biotechnol Bioeng 11:269–281

  3. Evans WC, Smith BSW, Fernley HN, Davies JI (1971) Bacterial metabolism of 2,4-dichlorophenoxyacetate. Biochem J 122:543–551

  4. Fradette S (1993) Comparaison de la respirométrie et de la calorimétrie comme outils d'évaluation de l'activité biologique dans les sols contaminés par l'herbicide 2,4-D. M.Sc. Thesis, Laval University

  5. Greer CW, Hawari J, Samson R (1990) Influence of physicochemical factors on the degradation of 2,4-dichlorophenoxy acetic acid by aPseudomonas sp. isolated from peat moss. Arch Microbiol 154:317–322

  6. Ishikawa Y, Nonoyama Y, Shoda M (1981) Microcalorimetric study of aerobic growth ofEscherichia coli in batch culture. Biotechnol Bioeng 23:2825–2836

  7. Kawabata T, Yamano H, Takahashi K (1983) An attempt to characterize calorimetrically the inhibitory effect of foreign substances on microbial degradation of glucose in soil. Agric Biol Chem 47:1281–1288

  8. Kimura T, Takahashi K (1985) Calorimetric studies of soil microbes: quantitative relation between heat evolution during microbial degradation of glucose and changes in microbial activity in soil. J Gen Microbiol 131:3083–3089

  9. Loos MA, Roberts RN, Alexander M (1967) Formation of 2,4-dichlorophenol and 2,4-dichloroanisole from 2,4-dichlorophenoxyacetate byArthrobacter sp. Can J Microbiol 13:691–699

  10. Lovrien RE, Ferry ML, Magnuson TS, Blanchette RA (1989) Microbial calorimetric analysis. ACS Symp Ser 399:544–558

  11. Luong JHT, Volesky B (1980) Determination of the heat of some aerobic fermentations. Can J Chem Eng 58:497–504

  12. Radjendirane V, Bhat MA, Vaidyanathan CS (1991) Affinity purification and characterization of 2,4-diehlorophenol hydroxylase fromPseudomonas cepacia. Arch Biochem Biophysics 288:169–176

  13. Rochkind ML, Blackburn JW, Sayler GS (1986) Microbial decomposition of chlorinated aromatic compounds. EPA report no. EPA/600/2-86/090

  14. Roy D, Samson R (1988) Investigation of growth and metabolism ofSaccharomyces cerevisiae (baker's yeast) using microcalorimetry and bioluminometry. J. Biotechnol 8:193–206

  15. Sand W, Schröter A, Fortnagel P, Bock E (1990) Differentiation of plasmid-containingEscherichia coli strains by microcalorimetry. J Microbiol Methods 12:247–251

  16. Sinton GL, Fan LT, Erickson LE, Lee SM (1986) Biodegradation of 2,4-D and related xenobiotic compounds. Enzyme Microb Technol 8:395–403

  17. Soil Survey Staff (1975) Soil taxonomy: a basic system of soil classification for making and interpreting soil surveys. USDA-SCS Agricultural Handbook 436. U.S. Government Printing Office, Washington, D.C.

  18. Sparling GP (1983) Estimation of microbial biomass and activity in soil using microcalorimetry. J Soil Sci 34:381–390

  19. Stockar U von, Marison IN (1989) The use of calorimetry in biotechnology. Adv Biochem Eng 40:93–136

  20. Yamano H, Takahashi K (1983) Temperature effect on the activity of soil microbes measured from heat evolution during the degradation of several carbon sources. Agric Biol Chem 47: 1493–1499

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Correspondence to A. LeDuy.

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Fradette, S., Rho, D., Samson, R. et al. Microcalorimetry as a diagnostic and analytical tool for the assessment of biodegradation of 2,4-D in a liquid medium and in soil. Appl Microbiol Biotechnol 42, 432–439 (1994). https://doi.org/10.1007/BF00902753

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  • Biomass
  • Biodegradation
  • Liquid Medium
  • Diagnostic Tool
  • Analytical Tool