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Photoreactivation of UV-damage in phage containing 5-Bromouracil-DNA

Zusammenfassung

Es wird eine neue Strahlenschutzwirkung des Cysteamins gegenüber photoreaktivierbaren und nicht-photoreaktivierbaren UV-Schäden bei 5-Bromuracilsubstituierten Phagen beschrieben. BU-T1 Phagen UV-bestrahlt in Anwesenheit von 0,01 molar Cysteamin zeigen eine normale Strahlenempfindlichkeit und nahezu normale Photoreaktivierbarkeit, die bei Abwesenheit des Schutzstoffes blockiert ist. Der Mechanismus der Desensibilisierung wird diskutiert.

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Literature

  1. Fox, E., andM. Meselson: Unequal photosensitivity of the two strands of DNA in bacteriophage. J. molec. Biol.7, 583–589 (1963).

  2. Harm, W.: On the relationship between host-cell reactivation and UV-reactivation in UV-inactivated phages. Z. Vererbungsl.94, 67–79 (1963).

  3. Hotz, G.: Suppression by cysteamin of radiosensitization in 5-bromodeoxyuridine substituted phage T1. Biochem. biophys. Res. Commun.11, 393–398 (1963).

  4. Hotz, G.: Unpublished results (1964).

  5. —, andK. G. Zimmer: Experiments in radiation chemistry of T1-phage. Int. J. Radiat. Biol.7, 75–86 (1963).

  6. Lochmann, E. R.: Die Umwandlung von 5-Bromuracil-2-14C durch Röntgenstrahlen in vitro und in vivo. Naturwissenschaften50, 520–521 (1963).

  7. Sauerbier, W.: The influence of 5-bromodeoxyuridine substitution on UV-sensitivity, host-cell reactivation, and photoreactivation in T1 and P22H5. Virology15, 465–472 (1961).

  8. Setlow, R. B., andW. L. Carrier: The disappearance of thymine dimers from DNA: An error-correcting mechanism. Proc. nat. Acad. Sci. (Wash.)51, 226–231 (1964).

  9. Smith, K. G.: Photochemistry of the nucleic acids. In:A. C. Giese's Photophysiology, vol. 2. New York and London: Acdemic Press 1964.

  10. Stahl, F. W., J. M. Crasemann, L. Okun, E. Fox, andC. Laird: Radiation-sensitivity of bacteriophage containing 5-bromodeoxyuridine. Virology13, 98–104.

  11. Wacker, A.: Molecular mechanisms of radiation effects. In:Davidson andColin's Progress in nucleic acid research, vol. 1, pp. 369–399. New York: Academic Press 1963.

  12. Wulff, D. L., andC. S. Rupert: Disappearance of thymine photodimer in ultraviolet irradiated DNA upon treatment with a photo-reactivating enzyme from baker's yeast. Biochem. biophys. Res. Commun.7, 237–240 (1962).

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Hotz, G. Photoreactivation of UV-damage in phage containing 5-Bromouracil-DNA. Zeitschrift für Vererbungslehre 95, 211–214 (1964). https://doi.org/10.1007/BF00897006

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Keywords

  • Cysteamin