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Effects of radioactive decay on the autonomous replicatin of anEscherichia coli episome

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Summary

TheE. coli episomeF 13 loses its capacity for autonomous replication when the integrity of the molecule is impaired by the decay of32P atoms incorporated into its polynucleotide chains. Upon further division of the host bacteria, the distal fragments near the episomal terminus are eliminated, whereas, the proximal fragments near the episomal origin may survive as heterogenotes attached to the host chromosome.

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References

  1. Adelberg, E. A., andS. N. Burns: Genetic variation in the sex factor ofEscherichia coli. J. Bact.79, 321 (1960).

  2. Cairns, J.: The bacterial chromosome and its manner of replication as seen by autoradiography. J. molec. Biol.6, 208 (1963).

  3. Cavalli, L. L., J. Lederberg, andE. M. Lederberg: An infective factor controlling sex compatibility inBacterium coli. J. gen. Microbiol.8, 89 (1953).

  4. Driskell-Zamenhof, P. J., andE. A. Adelberg: Studies on the chemical nature and size of sex factors ofEscherichia coli K 12. J. molec. Biol.6, 483 (1963).

  5. Echols, H., A. Garen, S. Garen, andA. Torriani: Genetic control of repression of alkaline phosphatase inE. coli. J. molec. Biol.3, 425 (1961).

  6. Fraser, D., andA. Jerrel: The amino acid composition of T3 bacteriophage. J. biol. Chem.205, 291 (1953).

  7. Fuerst, C. R., F. Jacob, etE. L. Wollman: Determination de liaisons genetiques chezE. coli K12, a l'aide du radiophosphore. C. R. Acad. Sci. (Paris)243, 2162 (1956).

  8. Hakura, A., andY. Hirota: Sex-determining temperate phagetau inE. coli K12. Jap. J. Genetics36, 379 (1961).

  9. Hanawalt, P. C., O. Maaløe, D. J. Cummings, andM. Schaechter: The normal DNA replication cycle. II. J. molec. Biol.3, 156 (1961).

  10. Hayes, W.: The mechanism of genetic recombination inE. coli. Cold Spr. Harb. Symp. quant. Biol.18, 75 (1953).

  11. Hershey, A. D., andN. Melechen: Synthesis of phage precursor nucleic acid in the presence of chloramphenicol. Virology3, 207 (1957).

  12. Hirota, Y.: Mutants of the sex factor inE. coli K12. Genetics44, 515 (1959).

  13. —, andP. H. A. Sneath: F' and F mediated transduction inEscherichia coli K12. Jap. J. Genetics36, 307 (1961).

  14. —, andH. Uchida: Mode of replication of chromosome inEscherichia coli. Jap. J. Genetics37, 386 (1962).

  15. Horiuchi, T., S. Horiuchi, andA. Novick: The genetic basis of hyper-synthesis of β-galactosidase. Genetics48, 157 (1963).

  16. Jacob, F., etE. A. Adelberg: Transfert de caracteres genetiques par incorporation au facteur sexuel d'Escherichia coli. C. R. Acad. Sci. (Paris)249, 189 (1959).

  17. —, etS. Brenner: Sur la regulation de la synthese du DNA chez les bacteries: l'hypothese du replicon. C. R. Acad. Sci. (Paris)256, 298 (1963).

  18. —, etE. L. Wollman: Les episomes, elements genetiques ajoutes. C. R. Acad. Sci. (Paris)247, 154 (1958).

  19. ——: Sur les processus de conjugaison et de recombinaison chezE. coli. IV. Prophages inductibles et mesures des segments genetiques transferes au cours de la conjugaison. Ann. Inst. Pasteur95, 497 (1958).

  20. Lederberg, J.: Isolation and characterization of biochemical mutants of bacteria. In: Methods in Medical Research, vol. 3, p. 5. Chicago: Year Book Publ. 1950.

  21. Loeb, F.: The isolation of a bacteriophage specific for the F+ and Hfr mating types ofE. coli K12. Science131, 932 (1960).

  22. Maaløe, O., andP. C. Hanawalt: The normal DNA replication cycle. I. J. molec. Biol.3, 144 (1961).

  23. Stent, G. S., andC. R. Fuerst: Inactivation of bacteriophages by decay of incorporated radioactive phosphorus. J. gen. Physiol.38, 441 (1955).

  24. ——: Genetic and physiological effects of the decay of incorporated radioactive phosphorus in bacterial viruses and bacteria. Advanc. biol. med. Phys.7, 1 (1960).

  25. Wollman, E. L., F. Jacob, andW. Hayes: Conjugation and genetic recombination inEscherichia coli. Cold Spr. Harb. Symp. quant. Biol.21, 141 (1956).

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Hirota, Y., Uchida, H. Effects of radioactive decay on the autonomous replicatin of anEscherichia coli episome. Zeitschrift für Vererbungslehre 95, 184–194 (1964). https://doi.org/10.1007/BF00894919

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Keywords

  • Radioactive Decay
  • Polynucleotide
  • Distal Fragment
  • Host Bacterium
  • Proximal Fragment