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Biological melting curves for the “replicative form” of ΦX 174 DNA

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Summary

The effect of heating and quick cooling on the infectivity of partially purified ΦX 174 replicative form was examined. A sharp increase, up to 300 fold, in infectivity was observed over a range of 4–8° C. The biologicalT M (defined as half-maximal infectivity) shows a linear dependence on the logarithm of ionic strength. The G-C content of the replicative form, as calculated from a comparison of the biologicalT M values with opticalT M values obtained byMarmur andDoty onE. coli DNA, is 45% (theoretical value=43%). The results provide a simple biological assay for the replicative form; they also indicate a high specificity of our competent bacteria for the heated replicative form.

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Benzinger, R., Hofschneider, P.H. Biological melting curves for the “replicative form” of ΦX 174 DNA. Zeitschrift für Vererbungslehre 94, 316–321 (1963). https://doi.org/10.1007/BF00894775

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Keywords

  • Ionic Strength
  • Linear Dependence
  • High Specificity
  • Sharp Increase
  • Biological Assay