The interpretation of experiments with HNO2-inactivated phage T4 published byHarm (1960) and of similar experiments with UV inactivated phages T2 and T4 (Dulbecco 1952,Harm 1956, andEpstein 1958) is discussed. Two alternative theories are used as possible interpretations of the HNO2 experiments: (1) The “injection damage” theory holding that a fractionJ of the damages caused in a phage by HNO2 treatment prevent participation of the phage in the infectious process. (2) The “finite damage” theory holding that the damages produced by HNO2 in the genetic material are much larger (have a greater target length) than UV damages, and otherways applying to HNO2 damages the same theory earlier applied to UV-damages (Barricelli 1956 and 1960).
Experimental methods to decide between the two theories are indicated. In the conclusion it is shown that the injection damage theory is not valid for UV damages in T2 and T4. In this case the theory is ruled out by the results ofDulbecco's (1952),Harm's (1956) andEpstein's (1958) MR experiments.
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Barricelli, N. A.: A “chromosomic” recombination theory for multiplicity-reactivation in phages. Acta biotheor. (Leiden)11, 107–120 (1956).
—: An analytical approach to the problems of phage recombination and reproduction. I. Multiplicity reactivation and the nature of radiation damages. Virology2, 99–135 (1960).
Barricelli, N. A.: A general survival law for virus and cells with damaged genetic material. (Virology 1961 in press.)
—, andA. H. Doermann: An analytical approach to the problems of phage recombination and reproduction. III. Crossreactivation. Virology13, 460–476 (1961).
Doermann, A. H., M. Chase andF. W. Stahl: Genetic recombination and replication in bacteriophage. J. cell. comp. Physiol.45, Suppl.2, 51–74 (1955).
Epstein, R. H.: A study of multiplicity-reactivation in the bacteriophage T4. Virology6, 382–404 (1958).
Harm, W.: On the mechanism of multiplicity-reactivation in bacteriophage. Virology2, 559–564 (1956).
—: Vergleichende Untersuchungen an HNO2-inaktivierten und UV-inaktivierten Bakteriophagen T 4. Z. Vererb.-Lehre91, 52–62 (1960).
Herriott, R. M., andJ. L. Barlow: The Protein Coats or “Ghosts” of Coli Phage T2. J. gen. Physiol.41, 307–331 (1957).
Krieg, D. R.: A study of gene action in ultraviolet-irradiated bacteriophage T4. Virology8, 80–98 (1959).
Luria, S. E.: Reactivation of irradiated bacteriophage by transfer of self-reproducing units. Proc. nat. Acad. Sci. (Wash.)33, 253–264 (1947).
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This investigation was supported by research grant RG-6980 from the Division of General Medical Sciences and C-4437 from the National Cancer Institute of the National Institutes of Health, U.S.A. Public Health Service.
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Barricelli, N.A., Avent, K. On the nature of HNO2 and UV damages in T-even bacteriophages. Zeitschrift für Vererbungslehre 92, 183–189 (1961). https://doi.org/10.1007/BF00890284
- Experimental Method
- Genetic Material
- Alternative Theory
- Infectious Process