Tritiated α-amanitin has been used as a specific and sensitive probe to estimate the number of RNA polymerase B molecules in isolated nuclei, chromatin and nucleoids, obtained from macroplasmodia ofPhysarum polycephalum. During mitosis (metaphase±10 min) there is at least 10-fold less RNA polymerase B than at all phases of the cell cycle, even if DNA replication has been blocked in vivo. It is concluded that many of the RNA polymerase B molecules leave the chromatin during decondensation of the chromosomes in telophase of the synchronous nuclear division ofPhysarum.
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Pierron, G., Sauer, H.W. RNA Polymerase B levels during the cell cycle ofPhysarum polycephalum . Wilhelm Roux' Archiv 189, 165–169 (1980). https://doi.org/10.1007/BF00868674
- RNA polymerase B
- [3H]α-amanitin binding
- Cell cycle