The effects were examined of 6-month intermittent hypobaric (4000 m) exposure on the antioxidant enzyme systems in soleus and tibialis muscles of rats. At the end of the 6-month experimental exposure, the six rats in both the exposed group and the control group were sacrificed. Immunoreactive mitochondrial superoxide dismutase (Mn-SOD) contents were measured as well as the activities of antioxidant enzymes [Mn-SOD, cytosolic SOD (Cu,Zn-SOD), catalase (CAT), and glutathione peroxidase (GPX)]. Thiobarbituric acid-reactive substances (TBARS) were also determined as an indicator of lipid peroxidation. The high altitude exposure resulted in a marked increase in TBARS content in soleus muscle, suggesting increased levels of oxygen free radicals. Conversely, significant decreases in both Mn-SOD content and activity in solens muscle were oted affer exposure. Such trends were not noticed in tibialis muscle. On the other hand, no significant changes in Cu,Zn-SOD, CAT, or GPX were observed in either muscle. These results suggested that the increases in lipid peroxidation were most probably a result of decreased Mn-SOD function which was more depressed in oxidative than in glycolytic muscle.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126
Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287
Costa LE (1990) Hepatic cytochrome P-450 in rats submitted to chronic hypobaric hypoxia. Am J Physiol 259: C654–C659
De Groot H, Littauer A (1989) Hypoxia, reactive oxygen and cell injury. Free Radic Biol Med 6:541–551
Dhalival H, Kirshenbaum LA, Randhawa AK, Signal PK (1991) Correlation between antioxidant changes during hypoxia and reoxygenation. Am J Physiol 261: H632–H638
Gregory EM, Fridovich I (1973) Oxygen toxicity and the superoxide dismutase. J Bacteriol 114:1197–1201
Hoshida S, Kuzuya T, Fuji H, Yamashita N, Oe H, Hori M, Suzuki K, Taniguchi N, Tada M (1993) Sublethal ischemia alters myocardial antioxidant activity in canine heart. Am J Physiol 264: H33–H39
Laughlin MH, Armstrong RB (1982) Muscular blood flow distribution patterns as a function of running speed in rats. Am J Physiol 243: H296–H306
Liu J, Simon LM, Phillips JR, Robin ED (1977) Superoxide dismutase (SOD) activity in hypoxic mamalian system. J Appl Physiol 42:107–110
Matsuda Y, Higashiyama S, Kijima Y, Suzuki K, Akiyama M, Kawano K, Kawata S, Tarui S, Deutsch HF, Taniguchi N (1990) Preparation, subunit structure and sulfhydryl reactivity of human manganase superoxide dismutase. Eur J Biochem 194:713–720
McCutchan HJ, Schwappach JR, Enquist EG, Walden DL, Terade LS, Reiss OK, Leef JA, Repine JE (1990) Xanthine oxidase-derived H2O2 contributes to reperfusion injury of ischemic skeletal muscle. Am J Physiol 258: H1415–H1419
Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358
Ohno H, Yahata T, Yamashita K, Yamamura K, Sato Y, Yamamoto C, Hayamizu S, Taniguchi N (1988) Effects of physical training on immunoreactive γ-glutamyltransferase in human plasma. Enzyme 39:110–114
Ohno H, Yamashita H, Ookawara T, Saito D, Wakabayashi K, Taniguchi N (1992) Training effects on concentrations of immunoreactive superoxide dismutase isoenzyme in human plasma. Acta Physiol Scand 146:291–292
Rice CL, Pettgrew FP, Noble EG, Taylor AW (1988) The fibre composition of skeletal muscle. In: Poortmans JR (ed) Principles of exercise biochemistry. Karger, Basel, pp 22–23
Sen CK, Ookawara T, Suzuki K, Taniguchi N, Hänninen O, Ohno H (1994) Immunoreactivity and activity of mitochondrial superoxide dismutase following training and exercise. Pathophysiology 00:1–4
Srivastava G, Bhatnagar R, Viswanathan R, Venkintasubramanian TA (1980) Microsomal and mitochondrial cytochromes in acutely hypoxic rat lung and liver. Ind J Biochem Biophys 17:130–134
Suzuki K, Nakata T, Seo HG, Miyazawa N, Sugiyama T, Taniguchi N (1991) A new model for hepatitis and liver cancer. In: Mori M, Yoshida MC, Takeichi N, Taniguchi N (eds) The EC rats. Springer, Berlin, Heidelberg, New York, Tokyo, pp 142–148
Suzuki K, Kinoshita N, Matsuda Y, Higashiyama S, Kuzuya T, Minamino T, Tada M, Taniguchi N (1992) Elevation of immunoreactive serum Mn-Superoxide dismutase in patients with acute myocardial infarction. Free Radic Res Commun 6:325–334
Taniguchi N (1992) Clinical significances of superoxide dismutases: changes in aging, diabetes, ischemia, and cancer. Adv Clin Chem 29:1–47
Tappel AL (1978) Glutathione peroxidase and hydroperoxidase. In: Fleischer S, Packer L (eds) Methods Enzymol 52:506–513
Turrens JF, Alexandre A, Lehninger AL (1985) Ubisemiquinone is an electron donor for superoxide formation by complex III of heart mitochondria. Arch Biochem Biophys 237:408–414
About this article
Cite this article
Radák, Z., Lee, K., Choi, W. et al. Oxidative stress induced by intermittent exposure at a simulated altitude of 4000 m decreases mitochondrial superoxide dismutase content in soleus muscle of rats. Europ. J. Appl. Physiol. 69, 392–395 (1994). https://doi.org/10.1007/BF00865401
- Intermittent exposure to high altitude
- Antioxidant enzymes
- Lipid peroxidation
- Fibre type
- Mitochondrial superoxide dismutase