Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Differential patterns in the temporal experession ofBombyx mori chorion genes


cDNA clones for two distinct families ofBombyx mori chorion protiens, Class A (m2774) and Class Hc (m5000), were used to study the developmental regulation of specific chorion mRNAs. Individual follicles from maturing ovarioles were assayed by Northern blotting techniques and amino acid pulse-labeling to determine concomitant RNA accumulation and protein synthesis patterns with a temporal resolution of 2.2–2.6 h of developmental age. RNAs hybridizing to the two cloned chorion sequences at high criterion showed distinctive, non-overlapping patterns of accumulation during the middle-late and very late stages of choriogenesis. Moreover, the periods of expression of these RNAs coincided exactly with the synthetic periods of two distinct subsets of chorion proteins, one containing Class A and B components, and the other containing Class Hc components alone. These results suggest that chorion gene transcription and translation are tightly coupled.

This is a preview of subscription content, log in to check access.



piperazine-N,N′-bis C2-ethane-sulfonic acid


sodium dodecyl sulfate




  1. Bock SC, Goldsmith MR (1979) Pattern and timing of chorion protein synthesis inBombyx mori strain C108. J Cell Biol 83:38a

  2. Bock SC, Tiemeier DC, Mester K, Wu M, Gerhart JC, Goldsmith MR (1980) RNA- and translational-level expression of specific chorion genes inBombyx mori strain C108. Am Zool 20:877a

  3. Bock SC, Tiemeier DC, Mester K, Wu M, Goldsmith MR (1982) Hybridization selected translation ofBombyx mori high cysteine chorion proteins inXenopus laevis oocytes. Proc Natl Acad Sci, USA 79:1032–1036

  4. Eickbush TH, Kafatos FC (1982) A Walk in the Chorion Locus ofBombyx mori. Cell 29:633–643

  5. Gelinas RE, Kafatos FC (1973) Purification of specific mRNAs from moth follicular cells. Proc Natl Acad Sci 70:3764–3768

  6. Gelinas RE, Kafatos FC (1977) The control of chorion protein synthesis in silkmoths: mRNA production parallels protein synthesis. Dev Biol 55:179–190

  7. Goldsmith MR, Basehoar G (1978) Organization of the chorion genes ofBombyx mori, a multigene family. I. Evidence for linkage to chromosome 2. Genetics 90:291–310

  8. Goldsmith MR, Clermont-Rattner E (1979) Organization of the chorion genes ofBombyx mori, a multigene family. II. Partial localization of three gene clusters. Genetics 92:1173–1185

  9. Goldsmith MR, Clermont-Rattner E (1980) Organization of the chorion genes inBombyx mori, a multigene family. III. Detailed composition of three gene clusters. Genetics 96:201–212

  10. Goldsmith MR, Paul M, Weare B, Clermont-Rattner E (1978) Development of the chorion inBombyx mori. J Cell Biol 79:320

  11. Goldsmith MR, Rattner EC, Koehler MMD, Balikov SR, Bock SC (1979) Two-dimensional electrophoresis of small-molecular weight proteins. Anal Biochem 99:33–40

  12. Grace TDC (1962) Establishment of four strains of cells from insect tissues grownin vitro. Nature (Lond) 195:788–789

  13. Iatrou K, Tsitilou SG, Goldsmith MR, Kafatos FC (1980). Molecular analysis of the GrB mutation inBombyx mori through the use of a chorion cDNA library. Cell 20:659–669

  14. Jones CW, Kafatos FC (1980) Structure, organization and evolution of developmentally regulated chorion genes in a silkmoth. Cell 22:855–867

  15. Kafatos FC, Efstratiadis A, Goldsmith MR, Jones CW, Maniatis T, Regier JC, Rodakis G, Rosenthal N, Sim GK, Thireos G, Villa-Komaroff L (1978) The developmentally regulated multigene families encoding chorion proteins in silkmoths. In: Ahmad F, Schults J, Russell TR, Werner R (eds) Differentiation and Development, Miami Winter Symposia, vol 15. Academic Press, New York, pp 299–315

  16. Laskey RA, Mills AD (1975) Quantitative film detection of3H and14C in polyacrylamide gels by fluorography. Eur J Biochem 56:335–341

  17. Lindquist S (1980) Varying patterns of protein synthesis inDrosophila during heat shock: Implications for regulation. Dev Biol 77:463–479

  18. Maniatis T, Jeffery A, Van de Sande H (1975) Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochem 14:3787–3794

  19. Nadel MR, Kafatos FC (1980) Specific protein synthesis in cellular differentiation. IV. The chorion proteins ofBombyx mori and their program of synthesis. Dev Biol 75:26–40

  20. Nadel MR, Goldsmith MR, Goplerud J, Kafatos FC (1980a) Specific protein synthesis in cellular differentiation. V. A. secretory defect of chorion formation in the Grcol mutant ofBombyx mori. Dev Biol 75:41–58

  21. Nadel MR, Thireos G, Kafatos FC (1980b) Effect of the pleiotropic GrB mutation ofBombyx mori on chorion protein synthesis. Cell 20:643–658

  22. Paul M, Goldsmith MR, Hunsley JR, Kafatos FC (1972) Specific protein synthesis in cellular differentiation: Production of eggshell proteins by silkmoth follicular cells. J Cell Biol 55:653–680

  23. Paul M, Kafatos FC (1975) Specific protein synthesis in cellular differentiation. II. The program of protein synthetic changes during chorion formation by silkmoth follicles, and its implementation in organ culture. Dev Biol 42:141–159

  24. Regier JC, Kafatos FC, Goodfliesh R, Hood L (1978) Silkmoth chorion proteins: Sequence analysis of the products of a multigene family. Proc Natl Acad Sci USA 75:390–394

  25. Rodakis GC (1978) PhD Thesis, University of Athens, Athens, Greece

  26. Rodakis GC, Kafatos FC (1982). The origin of evolutionary novelty in proteins; How a high-cysteine chorion protein has evolved. Proc Natl Acad Sci USA 79:3551–3555

  27. Rodakis GC, Moschonas NK, Kafatos FC (1982) Evolution of a multigene family of chorion proteins in silkmoths. Mol Cell Biol [in press]

  28. Sim GK, Kafatos FC, Jones CW, Koehler MD, Efstratiadis A, Maniatis T (1979) Use of a cDNA library for studies on evolution and developmental expression of the chorion multigene families. Cell 18:1303–1316

  29. Spradling AC, Mahowald AP (1979) Identification and genetic localization of mRNAs from ovarian follieles cells ofDrosophila melanogaster. Cell 16:589–598

  30. Takemoto T, Nagamatsu Y, Oka T (1980) Casein and α-lactalbumin messenger RNAs during development of the mouse mammary gland. Dev Biol 78:247–257

  31. Thireos G, Kafatos FC (1980) Cell-free translation of silkmoth chorion mRNAs: Identification of protein precursors and characterization of cloned cDNAs by hybrid-selected translation. Dev Biol 78:36–46

  32. Thireos G, Griffin-Shea R, Kafatos FC (1980) Untranslated mRNA for a chorion protein ofDrosophila melanogaster accumulates transiently at the onset of specific gene amplification. Proc Natl Acad Sci USA 77:5789–5793

  33. Thomson I, Wilkinson CE, Jackson JF, dePomerai DI, Clayton RM, Truman DES, Williamson R (1978) Isolation and cell-free translation of chick lens crystallin mRNA during normal development and transdifferentiation of neural retina. Dev Biol 65:372–382

Download references

Author information

Correspondence to Marian Ruth Goldsmith.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Bock, S.C., Tiemeier, D.C., Mester, K. et al. Differential patterns in the temporal experession ofBombyx mori chorion genes. Wilhelm Roux' Archiv 192, 222–227 (1983).

Download citation

Key words

  • Silkmoth chorion
  • Transcription
  • Northern analysis