It has been suggested that the immune system might figure prominently in the regulation of forelimb regeneration. However, neither the nature of this influence nor the aspect(s) of regeneration influenced are clearly known. The determination of which components of the immune system are indispensable for regeneration would be a logical first step in attempting to address such questions. This investigation, therefore, examined the effects of removing the spleen, a major lymphoid organ in the newt, upon the progress of regeneration. Splenectomies performed concomitantly with or after forelimb amputation failed to alter the time course of regeneration. Splenectomies, but not sham-splenectomies, performed prior to amputation reduced the time required to achieve successive stages of regeneration under some, but not all conditions, i.e., when performed 10–20 days before amputation, during the late fall and winter. Up until 35 days after amputation, no gross morphological distortions were observed as a result of splenectomy. It was concluded that the spleen is not required for regeneration to occur.
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Andrews A (1976) Immunostimulation as a modulator of regeneration and growth. Am Zool 16:260
Carlson BM (1974) Morphogenetic interactions between rotated skin cuffs and underlying stump tissues in regenerating axolotl forelimbs. Dev Biol 39:263–285
Carrel A (1921) Cicatrization of wounds. XII. Factors initiating regeneration. J Exp Med 34:425–434
Carrel A (1922) Growth-promoting function of leukocytes. J Exp Med 36:385–391
Carroll JM, Sicard RE (1979) Cyclic AMP phosphodiesterase activity in regenerating forelimbs of the adult newt. J Cell Biol 83:393a
Fidler I (1973) In vitro studies of cellular-mediated immunostimulation of tumor growth. J Nat Cancer Inst 50:1307–1312
Fini ME (1979) The effect of splenectomy upon forelimb regeneration in the adult newt,Notophthalmus viridescens. (MS Thesis, Boston College)
French V, Bryant PJ, Bryant SV (1976) Pattern regulation in epimorphic fields. Science 193:969–981
Hightower JA, St Pierre RL (1971) Hemopoietic tissue in the adult newt,Notophthalmus viridescens. J Morphol 135:299–308
Nouza K, Nemec P, Draber P (1973) The influence of host splenectomy on the tumor allograft-promoting effectiveness of antilymphocyte serum. Folia Biol Prague 19:267–272
Prehn RT (1970) Immunosurveillance, regeneration, and oncogenesis. Prog Exp Tumor Res 14:1–24
Prehn RT (1971) Perspectives on oncogenesis: does immunity stimulate or inhibit neoplasia? J Reticuloendothel Soc 10:1–16
Prehn RT (1972) The immune reaction as a stimulator of tumor growth. Science 176:170–171
Prehn RT, Lappé M (1971) An immunostimulation theory of tumor development. Transplant Rev 7:26–54
Schauble MK (1972) Seasonal variation of newt forelimb regeneration under controlled environmental conditions. J Exp Zool 181:281–286
Schauble MK, Tyler DB (1972) The effect of prolactin on the seasonal cyclicity of newt forelimb regeneration. J Exp Zool 182:41–46
Singer M (1978) On the nature of the neurotrophic phenomenon in urodele limb regeneration. Am Zool 18:829–842
Spärck J (1969) The function of the immune response in tumor growth and its genetical regulation. Arch Pathol Microbiol Scand 77:1–23
Stocum DL (1978) Organization of morphogenetic fields in the regenerating amphibian limb. Am Zool 18:883–896
Tank PW (1979) Positional information in the forelimb of the axolotl: experiments with double-half tissues. Dev Biol 73:11–24
Tassava RA, McCullough WD (1978) Neural control of cell cycle events in regenerating salamander limbs. Am Zool 18:843–854
Voisin GA (1971) Immunological facilitation — a broadening of the enhancement phenomenon. Prog Allergy 15:328–385
Portions of this work constitute part of the thesis submitted by M.E. Fini in partial fulfillment of the requirements for the M.S. degree in Biology at Boston College
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Fini, M.E., Sicard, R.E. Limb regeneration of the adult newt (Notophthalmus viridescens) in the absence of the spleen. Wilhelm Roux' Archiv 189, 77–79 (1980). https://doi.org/10.1007/BF00848570
- Immune system
- Notophthalmus viridescens