Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Sulfate and glucosamine labelling of the intercellular matrix in vitellogenic follicels of a moth

Summary

InHyalophora cecropia the intercellular spaces of the follicles contain during vitellogenesis a matrix that can be labelled eitherin situ or in culture with35S-sulfate and3H-glucosamine. The matrix was demonstrated by autoradiography and also by treating follicles for 15 min with pronase, which released TCA-soluble matrix fragments with molecular weights of up to 2×106 daltons. Testicular hyaluronidase degraded the high molecular weight fragments, and thus it is probable that they are chondroitin sulfate-like mucopolysaccharides. With the termination of vitellogenesis new matrix is no longer deposited, and the pre-existing material is disassembled. The sulfated matrix may account for the patency of the intercellular diffusion channels essential for blood protein uptake and also for the low level, extracellular binding of blood proteins that characterizes the vitellogenic follicle inHyalophora.

This is a preview of subscription content, log in to check access.

References

  1. Abu-Hakima, R., Davey, K.G.: The action of juvenile hormone on the follicle cells ofRhodnius prolixus: The importance of volume change. J. Exp. Biol.69, 33–44 (1977)

  2. Anderson, E.: Oocyte differentiation and vitellogenesis in the roach,Periplaneta americana. J. Cell Biol.20, 131–155 (1964)

  3. Anderson, L.M.: Protein synthesis and uptake by isolated Cecropia oocytes. J. Cell Sci.8, 735–750 (1971)

  4. Anderson, L.M., Telfer, W.H.: A follicle cell contribution to the yolk spheres of moth oocytes. Tiss. Cell1, 633–644 (1969)

  5. Anderson, L.M., Telfer, W.H.: Trypan blue inhibition of yolk deposition—a clue to follicle cell function in the Cecropia moth. J. Embryol. Exp. Morphol.23, 35–52 (1970a)

  6. Anderson, L.M., Telfer, W.H.: Extracellular concentrating of proteins in the Cecropia moth follicle. J. Cell. Physiol.76, 37–53 (1970b)

  7. Ashhurst, D.E.: The connective tissue of insects. Ann. Rev. Entomol.13, 45–74 (1968)

  8. Ashhurst, D.E., Costin, N.M.: Insect mucosubstances. I. The mucosubstances of developing connective tissue inLocusta migratoria. Histochem. J.,3, 279–295 (1971a)

  9. Ashhurst, D.E., Costin, N.M.: Insect mucosubstances II. The mucosubstances of the central nervous systems. Histochem. J.3, 297–310 (1971b)

  10. Ashhurst, D.E., Costin, N.M.: Insect mucosubstances. III. Some mucosubstances of the nervous system of the wax moth (Galleria mellonella) and the stick insect (Carausius morosus) Histochem. J.3, 379–387 (1971c)

  11. Ashhurst, D.E., Patel, N.G.: Hyaluronic acid in cockroach ganglia. Ann. Entomol. Soc. Am.56, 182–184 (1963)

  12. Bast, R., Telfer, W.: Follicle cell protein synthesis and its contribution to the yolk of the Cecropia moth oocyte. Develop. Biol.52, 83–97 (1976)

  13. Bonhag, P.F.: Ovarian structure and vitellogenesis in insects. Ann. Rev. Entomol.3, 137–160 (1958)

  14. Comper, W.D., Laurent, T.C.: Physiological function of connective tissue polysaccharides. Physiol. Rev.58, 255–315 (1978)

  15. Davey, K., Huebner, E.: The response of the follicle cells ofRhodnuis prolixus to juvenile hormone and antigonadotropin in vitro. Can. J. Zool.52, 1407–1412 (1974)

  16. DeLoof, A., Lagasse, A., Bohyn, W.: Proteid yolk formation in the Colorado beetle with special reference to the mechanism of the selective uptake of hemolymph proteins. Koninkl. Nederl. Akad. V. Wet. Ser. C.75, 125–143 (1974)

  17. Ehrhardt, Von P.: Die Rolle von Methionin, Cystein, Cystin and Sulfat bei der künstlichen Ernährung vonNeomyzus (Aulacorthum) circumflexus Bukt (Aphidae, Homoptera, Insecta). Biol. Zbl.88, 335–348 (1969)

  18. Estes, Z.E., Faust, R.M.: Studies on the mucopolysaccharides of the greater wax mothGalleria mellonella (Linnnaeus) Comp. Biochem. Physiol.13, 443–452 (1964)

  19. Hilchey, J.D., Block, R.J., Miller, L.P., Weed, R.M.: The sulfur metabolism of insects. I. The utilization of sulfate for the formation of cystine and methionine by the German cockroach,Blattella germanica (L). Contrib. Boyce Thompson Inst.18, 109–123 (1955)

  20. Hoglund, L.: The comparative biochemistry of invertebrate mucoploysaccharides. V. Insecta (Calliphora erythrocephala) Comp. Biochem. Physiol.,53(B), 9–14 (1976a)

  21. Hoglund, L.: Changes in mucopolysaccharides during the development of the blowfly,Calliphora erythrocephala. J. Insect Physiol.22, 917–924 (1976b)

  22. Lane, N.J., Treherne, J.E.: Uptake of peroxidase by the cockroach nervous system. Tiss. Cell2, 413–425 (1970)

  23. Laurent, T.C., Bjork, I; Pietruszkiewicz, A., Persson, H.: On the interaction between polysaccharides and other molecules. II. The transport of globular particles through hyaluronic acid solutions. Biochem. Biophys. Acta,78, 351–359 (1963)

  24. Melius, M.E., Telfer, W.H.: An autoradiographic analysis of yolk deposition in the Cecropia moth oocyte. J. Morphol.129, 1–16 (1969)

  25. Nisizawa, K., Yamaguchi, T., Handa, N., Maeda, M., Yamazaki, H.: Chemical nature of a uronic acid-containing polysaccharide in the peritrophic membrane of the silkworm. Biochem. J. (Tokyo)54, 419–426 (1963)

  26. Pan, M.L., Wallace, R.A.: Cecropia vitellogenin: isolation and characterization. Am. Zool.14, 1239–1242 (1974)

  27. Pratt, G.E., Davey, K.G.: The corpus allatum and oogenesis inRhodnius prolixus (Stal). J. Exp. Biol.56, 201–214 (1972)

  28. Roth, T.F., Porter, K.R.: Yolk protein uptake in the oocytes of the mosquitoAedes aegypti.J. Cell Biol. 20, 313–332 (1964)

  29. Rubenstein, E.: The role of an epithelial occlusion zone in the termination of vitellogenesis inHyalophora cecropia ovarian follicles. Develop. Biol. (in press) (1979)

  30. Stay, B.: Protein uptake in the oocytes of the Cecropia moth. J. Cell Biol.26, 49–62 (1965)

  31. Telfer, W. H.: The route of entry and localization of blood proteins in the oocyte of saturniid moths. J. Biophys. Biochem. Cytol.9, 747–759 (1961)

  32. Telfer, W.H., Anderson, L.M.: Changes associated with the initiation of a terminal growth phase in the oocyte of the Cecropia moth. Develop. Biol.17, 512–535 (1968)

  33. Telfer, W.H., Smith, D.S.: Aspects of egg formation. Symp. Royal Entomol. Soc. London5, 117–134 (1970)

  34. Treherne, J.E., Pichon, Y.: The insect blood-brain barrier. Adv. Insect Physiol.9, 257–313 (1972)

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Telfer, W.H. Sulfate and glucosamine labelling of the intercellular matrix in vitellogenic follicels of a moth. Wilhelm Roux' Archiv 185, 347–362 (1979). https://doi.org/10.1007/BF00848521

Download citation

Key words

  • Ovary
  • Silkworm
  • Sulfate
  • Glucosamine
  • Mucopolysaccharide