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The presence of an endogenous lectin in early embryos ofXenopus laevis

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Summary

Xenopus laevis embryos were examined for the presence of endogenous carbohydrate binding proteins. Soluble extracts of cleavage, gastrula and neurula embryos are able to agglutinate trypsinized rabbit erythrocytes. Unlike other embryonic lectins this agglutination activity requires the presence of calcium ions but not of sulphydryl reducing agents. It is specifically inhibited by galactose and galactose containing derivatives. Thiodigalactoside is the most potent disaccharide inhibitor followed by lactose and melibiose respectively. Methyl α-d-galactopyranoside is a more effective inhibitor than its β anomer. N-acetyl-d-galactosamine, N-acetyl-d-glucosamine, methyl α-d-mannopyranoside andl-fucose do not inhibit activity at concentrations at or above 25 mM. EDTA and EGTA are also strong inhibitors of this activity. β-d-galactoside binding lectins present in the early chick embryo have been implicated in cell to cell and cell to substrate adhesiveness of extraembryonic chick endoderm cells. Since cells of the blastula inXenopus laevis possess surface receptors bearing terminal β-d-galactoside groups it is possible that this β-d-galactoside binding lectin may play a role in the control of cell surface mediated events during development.

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References

  1. Barondes SH (1981) Lectins: Their multiple endogenous cellular functions. Ann Rev Biochem 50:207–231

  2. Barondes SH, Rosen SD (1976) Cell surface carbohydrate-binding proteins: Role in cell recognition. In: Barondes SH (ed) Neuronal recognition. Plenum Press, New York, pp. 331–356

  3. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

  4. Cook GMW, Zalik SE, Milos N, Scott V (1979) A lectin which binds specifically to β-d-galactoside groups is present at the earliest stages of chick embryo development. J Cell Sci 38:293–304

  5. Den H, Malinzak DA (1977) Isolation and properties of β-d-galactoside-specific lectin from chick embryo thigh muscle. J Biol Chem 252:5444–5448

  6. Fraser BR, Zalik SE (1977) Lectin mediated agglutination of amphibian embryonic cells. J Cell Sci 27:227–243

  7. Hamburger V (1960) A manual of experimental embryology. University of Chicago Press, Chicago

  8. Kitamura K (1980) The changes in lectin activity during the development of embryonic chick skin. J Embryol Exp Morphol 59:59–59

  9. Kobiler D, Barondes SH (1977) Lectin activity from embryonic chick brain, heart and liver: Changes with development. Dev Biol 60:326–330

  10. Milos N, Zalik SE (1982) Mechanisms of adhesion among cells of the early chick blastoderm. Role of the β-d-galactoside-binding lectin in the adhesion of extraembryonic endoderm cells. Differentiation 21:175–182

  11. Milos N, Zalik SE (1981) Effect of the β-d-galactoside-binding lectin on cell to substratum and cell to cell adhesion of cells from the extraembryonic endoderm of the early chick blastoderm. Wilhelm Roux's Arch 190:259–266

  12. Nieuwkoop PD, Faber J (1967) Normal table ofXenopus laevis (Daudin). North-Holland, Amsterdam

  13. Nowak TP, Kobiler D, Roel LE, Barondes SH (1977) Developmentally regulated lectin from embryonic chick pectoral muscle. J Biol Chem 252:6062–6030

  14. Podleski TR, Greenberg I, Nichols SC (1979) Studies on lectin activity during myogenesis. Exp Cell Res 122:305–316

  15. Rosen SD, Kafka JA, Simpson DL, Barondes SH (1973) Developmentally regulated carbohydrate-binding protein inDictyostelium discoideum. Proc Natl Acad Sci USA 70:2554–2557

  16. Wyrick RE, Nishihara T, Hedrick JL (1974) Agglutination of jelly coat and cortical granule components and the block to polyspermy in the amphibianXenopus laevis. Proc Natl Sci USA 71:2067–2071

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Correspondence to Sara E. Zalik.

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Lorena Harris, H., Zalik, S.E. The presence of an endogenous lectin in early embryos ofXenopus laevis . Wilhelm Roux' Archiv 191, 208–210 (1982). https://doi.org/10.1007/BF00848338

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Key words

  • Amphibian embryos
  • Endogenous lectin
  • Cell Surface