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Developmental potentials of the cells of the male foreleg disc ofDrosophila

I. Pattern regulation in intact fragments

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Summary

  1. 1.

    The developmental potentials of the four quadrants of the male foreleg disc ofDrosophila melanogaster were analysed by culturing excised quadrants for 3 days and 10 days in adult hosts prior to metamorphosis.

  2. 2.

    The cultured pieces underwent different types of pattern regulation in a circular direction. The upper medial piece was able to regenerate the missing structures of the disc, thus confirming the findings of earlier reports. The three remaining pieces could undergo pattern duplication in mirror-image symmetry. The lower medial piece revealed in addition a slight capacity for regeneration from the vertical cut surface.

  3. 3.

    The duplicating pieces differed markedly in their frequencies of pattern duplication: duplications occurred with very high frequencies in lower medial pieces, with intermediate frequencies in upper lateral pieces, and with very low frequencies in lower lateral pieces.

  4. 4.

    Both lower lateral and upper lateral pieces underwent a progressive loss of most markers with increasing culture time.

  5. 5.

    Claws were regenerated solely by upper medial pieces.

  6. 6.

    Transdetermined structures, too, were encountered only in upper medial pieces.

  7. 7.

    The results are discussed with respect to the two major current models of pattern regulation in imaginal discs, the “gradient model” and the “clock model”.

  8. 8.

    It is suggested that the differences in the frequencies of pattern duplication reflect the unequal spacing of circular positional values within the three duplicating quadrants. Under this assumption the data indicate a progressive decrease in the density of circular positional values with increasing distance from the upper medial quadrant of the disc.

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References

  1. Bryant, P. J.: Regeneration and duplication following operations in situ on the imaginal discs ofDrosophila melanogaster. Develop. Biol26, 606–615 (1971)

  2. Bryant, P.J.: Pattern formation in the imaginal wing disc ofDrosophila melanogaster: Fate map, regeneration and duplication. J. Exp. Zool193, 49–78 (1975)

  3. French, V., Bryant, P.J., Bryant, S.V.: Pattern regulation in epimorphic fields. Science193, 969–981 (1976)

  4. Garcia-Bellido, A.: Pattern reconstruction by dissociated imaginal disc cells ofDrosophila melanogaster. Develop. Biol.14, 278–306 (1966a)

  5. Garcia-Bellido, A.: Changes in selective affinity following transdetermination in imaginal disc cells ofDrosophila nelanogaster. Exp. Cell Res.44, 382–392 (1966b)

  6. Garcia-Bellido, A.: Histotypic reaggregation of dissociated imaginal disc cells ofDrosophila melanogaster cultured in vivo. Wilhelm Roux' Arch.158, 212–217 (1967)

  7. Garcia-Bellido, A.: Cell affinities in antennal homeotic mutants ofDrosophila melanogaster. Genetics59, 487–499 (1968)

  8. Garcia-Bellido, A., Lewis, E. B.: Autonomous cellular differentiation of homeotic bithorax mutants ofDrosophila melanogaster. Develop. Biol.48, 400–410 (1976)

  9. Garcia-Bellido, A., Nöthiger, R.: Maintenance of determination by cells of imaginal discs ofDrosophila after dissociation and culture in vivo. Wilhelm Roux's Archives180, 189–206 (1976)

  10. Hadorn, E.: Problems of determination and transdetermination. In “Genetic control of differentiation”, Brookhaven Symp. Biol.18, 148–161 (1965)

  11. Lee, L.-W., Gerhart, J.C.: Dependence of transdetermination frequency on the developmental stage of cultured imaginal discs ofDrosophila melanogaster. Develop. Biol.35, 62–82 (1973)

  12. Nöthiger, R.: Differenzierungsleistungen in Kombinaten, hergestellt aus Imaginalscheiben verschiedener Arten, Geschlechter und Körpersegmente vonDrosophila. Wilhelm Roux' Arch.155, 269–301 (1964)

  13. Poodry, C.A., Bryant, P.J., Schneiderman, H.A.: The mechanism of pattern reconstruction by dissociated imaginal discs ofDrosophila melanogaster. Develop. Biol.26, 464–477 (1971)

  14. Schubiger, G.: Anlageplan, Determinationszustand und Transdeterminationsleistungen der männlichen Vorderbeinscheibe vonDrosophila melanogaster. Wilhelm Roux' Arch.160, 9–40 (1968)

  15. Schubiger, G.: Regeneration, duplication and transdetermination in fragments of the leg disc ofDrosophila melanogaster. Develop. Biol.26, 277–295 (1971)

  16. Schubiger, G.: Regeneration ofDrosophila melanogaster male leg disc fragments in sugar fed female hosts. Experientia29, 631 (1973)

  17. Tobler, H.: Zellspezifische Determination und Beziehung zwischen Proliferation und Transdetermination in Bein- und Flügelprimordien vonDrosophila melanogaster. J. Embryol. exp. Morph.16, 609–633 (1966)

  18. Ulrich, E.: Cell lineage, Determination und Regulation in der weiblichen Genitalimaginalscheibe vonDrosophila melanogaster. Wilhelm Roux' Arch.167, 64–82 (1971)

  19. Van der Meer, J. M., Ouweneel, W.J.: Differentiation capacities of the dorsal metathoracic (haltere) disc ofDrosophila melanogaster. II. Regeneration and duplication. Wilhelm Roux' Arch.174, 361–373 (1974)

  20. Weber, K., Nöthiger, R.: DNA-synthesis in larval salivary glands during culture in starved female flies ofDrosophila melanogaster. Wilhelm Roux' Arch.176, 253–266 (1975)

  21. Wolpert, L.: Positional information and pattern formation. Current Topics in Develop. Biol.6, 183–224 (1971)

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Correspondence to Siegward Strub.

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Strub, S. Developmental potentials of the cells of the male foreleg disc ofDrosophila . Wilhelm Roux' Archiv 181, 309–320 (1977). https://doi.org/10.1007/BF00848058

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Key words

  • Drosophila melanogaster
  • Male foreleg disc
  • Pattern regulation