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Electrophoretic protein analysis for the identification of doubled haploid 1A–1R, 1B–1R wheat-rye double translocation lines and for the assessment of their genetic stability

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Abstract

Eighteen available doubled haploid wheat lines with a cytologically proven 1A–1R, 1B–1R double translocation, which where derived via anther culture from four crosses of the 1A–1R wheat-rye translocation cv “Amigo” with several 1B–1R wheat-rye translocation forms, were subjected to electrophoretic seed protein analysis. Besides, the five parents used in the crosses and some other wheat cultivars and doubled haploid lines (19 with a 1B–1R single translocation, 10 with a 1A–1R translocation and 7 without any 1R translocation) were also included in the investigation. It was found that the gliadin patterns visualized after SDS polyacrylamide gel electrophoresis of alcohol-soluble seed protein extracts can differentiate not only 1B–1R and 1A–1R translocation forms from wheats without any 1R-translocation chromosome, but also 1B–1R and 1A–1R wheats from each other. Moreover, 1A–1R, 1B–1R double translocation lines can be distinguished as well due to characteristic differences revealed between 1A–1R and 1B–1R translocation forms. Thus, all of tested dh1- and dh2-grains of the double translocation lines showed the expected doublet: the 1A–1R translocation (“Amigo”)-typical rye band and the 1B–1R translocation (“Kawkas”)-typical rye band. Consequently, gliadin patterns estimated after SDS electrophoresis may be used as markers for the fast detection of the desired 1A–1R, 1B–1R double translocation forms among 1A–1R single translocation lines, 1B–1R single translocation lines and lines without any 1R-translocation in the progenies of appropriate crosses. Furthermore, by means of gliadin tests on the dh2-generation the excellent stability of the double translocation 1A–1R, 1B–1R during more than one propagation phase has been proven. Estimations of high-molecular weight (HMW) glutenin subunits coded by 1A and 1B chromosomes are compatible with the double translocation constitution. A few deviating results can be explained by crossing-over events. Seed protein analysis revealed that it is possible to produce 1A–1R, 1B–1R double translocation lines with good glutenin compositions provided that adequate favourable parents are used.

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Former name: Department of Physiology of Institute for Cereal Research

Communicated by G. Wenzel

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Vahl, U., Müller, G. & Böhme, T. Electrophoretic protein analysis for the identification of doubled haploid 1A–1R, 1B–1R wheat-rye double translocation lines and for the assessment of their genetic stability. Theoret. Appl. Genetics 86, 547–556 (1993). https://doi.org/10.1007/BF00838707

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Key words

  • Gliadin
  • HMW glutenin
  • SDS electrophoresis
  • Biochemical marker
  • 1A-1R, 1B–1R wheatrye double translocation
  • Doubled haploids
  • Triticum aestivum L.