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Cultivation of human leukemia factor on the chorioallantoic membrane of chick embryos

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Allantoic fluid of chick embryos, infected by blood and filtrate of human brain tissue from patients with leukemia or persons dead from it, was injected to mice of low-leukemic breeds. It was demonstrated that the above substances cause the appearance of leukemia in 20.5 and 15% of animals. Allantoic fluid of chick embryos infected with blood of healthy persons caused leukemia only in 2.7% of mice, while allantoic fluid of embryos, infected by filtrate of normal brain tissue did not possess leukosogenic activity. Consequently, it is possible to cultivate the human factor for a long period of time on membranes of developing chick embryos (15 inoculations for over 3 months).

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Literature Cited

  1. [1]

    V. M. Bergol'ts, Problemy Gematol. No. 1, 11–18 (1957).

  2. [2]

    V. M. Bergol'ts and L. V. Shershul'skaia, Biull. Eksptl. Biol. i Med. 45, No. 5, 84–89 (1958). Original Russian pagination. See C. B. translation.

  3. [3]

    V. I. Ioffe et al., Zhur. Mikrobiol., Epidemiol. i Immunobiol. No. 7, 43–51 (1951).

  4. [4]

    F. I. Leikina,Ibid No. 10, 95–96 (1954).

  5. [5]

    A. D. Timofeevskii, Arkh Patol. No. 8, 14–21, (1956). Proceedings of a Scientific Conference of the Institute of Experimental Pathology and Therapy of Cancer, p. 3–4, Moscow, 1957. In Russian.

  6. [6]

    W. L. Bostick, J. Immunol., 1948, v. 59, 8, p. 189–193.

  7. [7]

    J. Brachet and J. Shaver, Experientia, 1949, v. 5, p. 235. Proc. Soc. Exper. Biol. a Med., 1952, v. 6, p. 173–200.

  8. [8]

    L. Dunham and H. L. Stewar, J. Nat. Cancer Inst. 1953 v. 13, p. 1299–1378.

  9. [9]

    R. Gentry and B. Burmester, Poultry Sci. 1955, v. 34, p. 669–672.

  10. [10]

    A. Graffi et al., Naturwissenschaften. 1955, Bd. 42, S. 421–442.

  11. [11]

    D. Lush, Australian J. Exper. Biol. a Med. Sci., 1937, v. 15, p. 131–139.

  12. [12]

    R. de Ruyck, Proc. Am. Assoc., Cancer Res. 1954, .p. 41.

  13. [13]

    M. H. Smith, Proc. Soc. exper. Biol. a. Med. 1948, v. 69, p. 136–140.

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Bergol'ts, V.M. Cultivation of human leukemia factor on the chorioallantoic membrane of chick embryos. Bull Exp Biol Med 46, 849–853 (1958).

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  • Public Health
  • Leukemia
  • Brain Tissue
  • Human Brain
  • Human Factor