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Prosaposin and prosaptide, a peptide from prosaposin, induce an increase in ganglioside content on NS20Y neuroblastoma cells

Abstract

Prosaposin has been recently identified as a neurotrophic factor eliciting differentiation in neuronal cultured cells (NS20Y). In this paper we investigate whether prosaposin and its active peptide (prosaptide) may modify the ganglioside pattern in neuroblastoma cells. The analysis by high performance thin layer chromatography did not reveal qualitative changes in the ganglioside pattern of NS20Y cells incubated in the presence of prosaposin, compared to control cells, but it did reveal an increase of the content of all three major resorcinol positive bands (GM3, GM2, GD1a). Cytofluorimetric and immunofluorescence microscopic analysis revealed that the increase of the ganglioside content was at the plasma membrane level. These findings suggest that the neurotrophic activity of prosaposin on NS20Y neuroblastoma cells might be mediated in part by the increase of cell surface gangliosides.

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References

  1. 1.

    Hakomori S (1993)Biochem Soc Trans 21: 583–95.

  2. 2.

    Hannun YA, Bell RM (1989)Science 243: 500–7.

  3. 3.

    Chigorno V, Pitto M, Cardace G, Acquotti D, Kirschner G, Sonnino S, Ghidoni R, Tettamanti G (1985)Glycoconjugate J 2: 279–91.

  4. 4.

    Bremer EG (1994)Current Topics in Membranes 40: 387–411.

  5. 5.

    Bremer EG, Schlessinger J, Hakomori S (1986)J Biol Chem 261: 2434–40.

  6. 6.

    Rosner H, Al-Aqtum M, Rahmann H (1992)Neurochem Int 20: 339–51.

  7. 7.

    Schengrund CL (1990)Brain Res Bulletin 24: 131–41.

  8. 8.

    Thangnipon W, Balazs R (1992)Neurochem Res 17: 45–59.

  9. 9.

    Carine K, Schengrund CL (1984)J Neurosc Res 12: 59–69.

  10. 10.

    Leon A, Facci L, Toffano G, Sonnino S, Tettamanti G (1981)J Neurochem 37: 350–57.

  11. 11.

    Kreutter D, Kim JYK, Goldenring JR, Rasmussen H, Ukomadu C, De Lorenzo RJ, Yu RK (1987)J Biol Chem 262: 1633–37.

  12. 12.

    Markwell MA, Fredman P, Svennerholm L (1984)Biochim Biophys Acta 775: 7–16.

  13. 13.

    Fishman PH (1982)J Membr Biol 69: 85–97.

  14. 14.

    Hiraiwa, M, O'Brien JS, Kishimoto Y, Galdzicka M, Fluharty AL, Ginns EI, Martin BM (1993)Arch Biochem Biophys 304: 110–16.

  15. 15.

    Fu Q, Carson GS, Hiraiwa M, Grafe M, Kishimoto Y, O'Brien JS (1994)J Molec Neurosc 5: 59–67.

  16. 16.

    Hiraiwa M, Soeda S, Kishimoto Y, O'Brien JS (1992)Proc Natl Acad Sci USA 89: 11254–58.

  17. 17.

    O'Brien JS, Carson GS, Seo HC, Hiraiwa M, Kishimoto Y (1994)Proc Natl Acad Sci USA 91: 9593–96.

  18. 18.

    O'Brien J, Carson GS, Seo HC, Hiraiwa M, Weiler S, Tomich JM, Barranger JA, Kahn M, Azuma N, Kishimoto Y (1995)FASEB J 9 681–85.

  19. 19.

    Uemura KE, Sugiyama E, Taketomi T (1991)J Biochem 110: 96–102.

  20. 20.

    Campana WC, Carson GS, Misasi R, O'Brien JS (1995)J Neurochem 64: PS24.

  21. 21.

    Svennerholm L, Fredman P (1980)Biochim Biophys Acta 617: 97–109.

  22. 22.

    Williams MA, McCluer RH (1980)J Neurochem 35: 266–69.

  23. 23.

    Svennerholm L (1957)Biochim Biophys Acta 24: 604–11.

  24. 24.

    Jourdian GW, Dean L, Roseman S (1971)J Biol Chem 246: 430–35.

  25. 25.

    Kotani M, Ozawa H, Kawashima I, Ando S, Tai T (1992)Biochim Biophys Acta 1117: 97–103.

  26. 26.

    Kishimoto Y, Hiraiwa M, O'Brien JS (1992)J Lipid Research 33: 1255–67.

  27. 27.

    Morimoto S, Yamamoto Y, O'Brien JS, Kishimoto Y (1990)Proc Natl Acad Sci USA 87: 3493–97.

  28. 28.

    Nores GA, Dohi T, Taniguchi M, Hakomori S (1987)J Immunol 139: 3171–76.

  29. 29.

    Rosner H, Greis CH, Rodemann HP (1990)Exp Cell Res 190: 161–69.

  30. 30.

    Saito M, Saito M, Rosenberg A (1984)Biochemistry 23: 1043–46.

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Misasi, R., Sorice, M., Carson, G.S. et al. Prosaposin and prosaptide, a peptide from prosaposin, induce an increase in ganglioside content on NS20Y neuroblastoma cells. Glycoconjugate J 13, 195–202 (1996). https://doi.org/10.1007/BF00731494

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Keywords

  • gangliosides
  • prosaposin prosaptide
  • neuroblastoma cells
  • differentiation