Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Mass spectrometric analysis of genetic and post-translational heterogeneity in the lectins Jacalin andMaclura pomifera agglutinin

  • 46 Accesses

  • 14 Citations


Jacalin andM. pomifera agglutinin are T-antigen specific lectins with α4β4 structures that show far greater microheterogeneity than plant lectins from other families, due to multiple genetic isoforms and post-translational processing. Electrospray mass spectrometry and combined liquid chromatography-electrospray mass spectrometry were used to characterize the various forms. For both lectins, the mass data were consistent with previous protein sequencing of the major α-chain species of 133 residues and three β-chain species of 20 or 21 residues. In addition, for jacalin the mass of one minor α-chain species was consistent with a second of the four reported gene sequences. However, the glycopeptide α-chain form and one β-chain form did not match any of the genes, suggesting a fifth gene remains to be found. ForM. pomifera agglutinin, three more β-chain forms were found, but all six could arise from only two genes, with additional post-translational proteolysis and post-translational substitution with an unidentified component of 106 Da creating the set of six forms. Only two α-chain forms were found also, with no glycosylation.

This is a preview of subscription content, log in to check access.


  1. 1.

    Becker JW, Reeke GN, Wang JL, Cunningham BA, Edelman GM (1975)J Biol Chem 259:1513–24.

  2. 2.

    Wright CS (1977)J Mol Biol 141:439–57.

  3. 3.

    Young NM, Johnston RAZ, Szabo AG, Watson DC (1989)Arch Biochem Biophys 270:596–603.

  4. 4.

    Lee X, Johnston RAZ, Rose DR, Young NM (1989)J Mol Biol 210:685–86.

  5. 5.

    Ruffet E, Paquet N, Frutiger S, Hughes GJ, Jaton J-C (1992)Biochem J 286:131–34.

  6. 6.

    Young NM, Johnston RAZ, Watson DC (1991)FEBS Letts 282:382–84.

  7. 7.

    Capon C, Piller F, Wieruszeski J-M, Leroy Y, Fournet B (1990)Carbohydr Res 199:121–27.

  8. 8.

    Ashford DA, Dwek RA, Rademacher TW, Lis H, Sharon N (1991)Carbohydr Res 213:215–27.

  9. 9.

    Yang H, Czapla TH (1993)J Biol Chem 268:5905–10.

  10. 10.

    Smith RD, Loo JA, Edmonds CG, Barinaga CJ, Udseth HR (1990)Anal Chem 62:882–89.

  11. 11.

    Tang X-J, Thibault P, and Boyd RK (1993)Anal Chem 65:2824–34.

  12. 12.

    Higgins TJV, Chandler PM, Zurawski E, Button SC, Spencer D (1983)J Biol Chem 258:9544–49.

  13. 13.

    Carrington DM, Auffret A, Hanks DE (1985)Nature 313:64–67.

  14. 14.

    Abe Y, Shirane K, Yokosawa H, Matsushita H, Mitta M, Kato I, Ishii S-I (1993)J Biol Chem 268: 3525–29.

  15. 15.

    Banerjee R, Dhanaraj V, Mahanta SK, Surolia A, Vijayan M (1991)J Mol Biol 221: 773–76.

Download references

Author information

Correspondence to N. Martin Young.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Young, N.M., Watson, D.C. & Thibault, D.P. Mass spectrometric analysis of genetic and post-translational heterogeneity in the lectins Jacalin andMaclura pomifera agglutinin. Glycoconjugate J 12, 135–141 (1995). https://doi.org/10.1007/BF00731357

Download citation


  • electrospray mass spectrometry
  • lectin
  • microheterogeneity
  • post-translational processing
  • sequencing