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Single mid-chain GlcNAcβ1-6Galβ1-4R sequences of linear oligosaccharides are resistant to endo-β-galactosidase ofBacteroides fragilis

Abstract

Endo-β-galactosidase (EC 3.2.1.103) ofBacteroides fragilis, at 250 mU ml−1, did not cleave the internal galactosidic linkage of the linear radiolabelled trisaccharide GlcNAcβ1-6Galβ1-4GlcNAc, or those of the tetrasaccharides Galβ1-4GlcNAcβ1-6Galβ1-4GlcNAc and Galβ1-4GlcNAcβ1-6Galβ1-4Glc. The isomeric glycans which contained the GlcNAcβ1-3Galβ1-4GlcNAc/Glc sequence were readily cleaved.

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Abbreviations

GlcNAc:

2-acetamido-2-deoxy-d-glucose

Lact:

lactose

MT:

maltotriose

MTet:

maltotetraose

R MTet :

chromatographic migration rate in relation to that of maltotetraose

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Correspondence to Ossi Renkonen.

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Renkonen, O., Penttilä, L., Niemelä, R. et al. Single mid-chain GlcNAcβ1-6Galβ1-4R sequences of linear oligosaccharides are resistant to endo-β-galactosidase ofBacteroides fragilis . Glycoconjugate J 8, 376–380 (1991). https://doi.org/10.1007/BF00731351

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Keywords

  • endo-β-galactosidase
  • Bacteroides fragilis
  • oligo-N-acetyllactosaminoglycans
  • GlcNAcβ1-6Galβ1-4R sequences