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Enzyme-linked immunosorbent assays for the measurement of blood group A and B glycosyltransferase activities

Abstract

ELISA assays have been developed for α(1–3)N-acetylgalactosaminyltransferase (blood group A transferase) and α(1–3)galactosyltransferase (blood group B transferase) activities. In these assays, microtitre plates coated with the bovine serum albumin conjugate of a synthetic Fucα1–2Galβ-R acceptor substrate are incubated with the appropriate nucleotide donor (UDP-GalNAc or UDP-Gal) and human serum as the enzyme source. The resulting trisaccharide products Fucα1–2(GalNAcα1–3)Galβ-R-BSA or Fucα1–2(Galα1–3)Galβ-R-BSA are detected and quantified with monoclonal antibodies selected not to cross-react with the substrate structure. With less than a microliter of human serum, product formation is proportional to enzyme concentration and to time of incubation of up to 90 min.

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Correspondence to Monica M. Palcic.

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Keshvara, L.M., Newton, E.M., Good, A.H. et al. Enzyme-linked immunosorbent assays for the measurement of blood group A and B glycosyltransferase activities. Glycoconjugate J 9, 16–20 (1992). https://doi.org/10.1007/BF00731173

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Keywords

  • ELISA
  • blood group AN-acetylgalactosaminyltransferase
  • blood group B galactosyltransferase
  • glycosyltransferase assays