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Time-resolved chlorophyll fluorescence of spruce needles under various light conditions

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Different branches of a declined spruce were exposed to full sunlight, reduced sunlight (using a fine wire mesh), and natural shadow. Subnanosecond decay kinetics and time-gated fluorescence spectra of individual needles were measured and compared with their chlorophyll concentration. Sunlight-exposed needles showed lower chlorophyll concentrations and higher intensitiesI 3 of a long-lived fluorescent component (decay time about 3 ns) than shadow needles. This seems to be due to a reduced energy transfer from the chlorophyll antenna molecules to the reaction centres of Photosystem II. After light reduction to 15–20% during one summer season the chlorophyll concentration increased, whereasI 3 decreased, thus proving some recovery of the photosynthetic apparatus.

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  1. 1.

    D. Siefermann-Harmset al. (1993)KfK-PEF 104, 79–126.

  2. 2.

    A. R. Holzwarth (1988) in H. K. Lichtenthaler (Ed.),Applications of Chlorophyll Fluorescence, Kluwer, Dordrecht, pp. 21–31.

  3. 3.

    H. Schneckenburger and W. Schmidt (1992)Radiat. Environ. Biophys. 31, 73–81.

  4. 4.

    E. H. Evans, R. G. Brown, A. R. Wellburn, and J. Wolfenden (1992)New Phytol. 122, 501–506.

  5. 5.

    H. Schneckenburger, K. König, T. Dienersberger, and R. Hahn (1994)Opt. Eng. 33, 2600–2606.

  6. 6.

    H. K. Lichtenthaler and A. R. Wellburn (1983)Biochem. Soc. Transact. 603, 591–592.

  7. 7.

    C. Buschmann (1986)Naturwissenschaften 73, 691–699.

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Correspondence to Herbert Schneckenburger.

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Schneckenburger, H., Schmidt, W. Time-resolved chlorophyll fluorescence of spruce needles under various light conditions. J Fluoresc 5, 155–158 (1995). https://doi.org/10.1007/BF00727532

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Key words

  • Forest decline
  • chlorophyll fluorescence
  • light stress
  • time-resolved spectroscopy