Paraffin-embedded material of 47 ovarian tumours primarily diagnosed as granulosa cell tumours, including 2 cases of juvenile granulosa cell tumour, were studied immunohistochemically for the presence of intermediate filament proteins, epithelial membrane antigen and tumour markers. Forty-one lesions, including the 2 juvenile granulosa cell tumours, were vimentin positive, while keratin and epithelial membrane antigen expression could not be detected. Six tumours primarily diagnosed as poorly differentiated malignant granulosa cell tumours were vimentin negative, showed a mild to moderate positivity for keratin and intense positivity with the anti-epithelial membrane antigen antibody. These latter tumours were therefore classified as undifferentiated ovarian carcinomas, corresponding to their significantly poorer prognosis and shorter survival when compared with the granulosa cell tumours. Two of these six tumours were positive for carcino-embryonic antigen. Two small cell carcinomas of the ovary studied in addition expressed keratin in a proportion of tumour cells while no epithelial membrane antigen or vimentin was detectable. None of the tumours tested for alpha-fetoprotein, human chorionic gonadotrophin, human placental alkaline phosphatase and human placental lactogen, were positive.
The data indicate the value of antibodies directed against intermediate filament proteins and epithelial membrane antigen to distinguish granulosa cell tumours from poorly differentiated carcinomas, a worthwhile distinction considering the much better prognosis of granulosa cell tumours.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Benfield GFA, Tapper-Jones L, Stont TV (1982) Androblastoma and raised serum AFP with familial multinodular goitre. Case report. Br J Obstet Gynaecol 89:323–326
Bonazzi del Pogetto C, Virtanen I, Lehto VP, Wahlstrom T, Sakscha E (1983) Expression of intermediate filaments in ovarian and uterine tumors. Int J Gynecol Pathol 1:359–366
Brown DC, Theaker JM, Banks PM, Gatter KC, Mason DY (1987) Cytokeratin expression in smooth muscle and smooth muscle tumors. Histopathology 11:477–486
Chadha S, Honnebier WJ, Schaberg A (1987) Raised serum alpha-fetoprotein in Sertoli-Leydig cell tumor (androblastoma) of ovary: Report of two cases. Int J Gynecol Pathol 6:82–88
Chumas JC, Rosenwaks Z, Mann WJ, Finkel G, Pastore J (1984) Sertoli-Leydig cell tumor of the ovary producing alpha-fetoprotein. Int J Gynecol Pathol 3:213–219
Czernobilsky B, Moll R, Leppien G, Schweikhart G, Franke WW (1987) Desmosomal plaque-associated vimentin filaments in human ovarian granulosa cell tumors of various histologic patterns. Am J Pathol 126:476–486
Delsol G, Gatter KC, Stein H, Erber WN, Pulford KAF, Zinne K, Mason DY (1984) Human lymphoid cells may express epithelial membrane antigens. Implications for the diagnosis of human neoplasms. Lancet 11:1124–1129
Fox H, Langley FA (1976) Tumours of the ovary. London, Heinemann
Fox H (1985) Sexcord-stromal tumours of the ovary. J Pathol 145:127–148
Kurman RJ, Ganjei P, Nadji M (1984) Contributions of immunohistochemistry to the diagnosis and study of ovarian neoplasms. Int J Gynecol Pathol 3:3–26
Mann WJ, Chumas J, Rosenwaks Z, Merril JA, Davenport D (1986) Elevated serum alpha-fetoprotein associated with Sertoli-Leydig cell tumors. Obstet Gynecol 67:141–144
Miettinen M, Letho VP, Virtanen I (1983) Expression of intermediate filaments in normal ovaries and ovarian epithelial sexcord-stromal and germinal tumors. Int J Gynecol Pathol 2:64–71
Miettinen M, Walstrom T, Vitranen I, Talerman A, Astengo-Osuna C (1985) Cellular differentiation in ovarian sexcord-stromal and germ-cell tumors studied with antibodies to intermediate-filament proteins. Am J Surg Pathol 9:640–651
Norton AJ, Thomas JA, Isaacson PG (1987) Cytokeratin-specific monoclonal antibodies are reactive with tumours of smooth muscle derivation. An immunocytochemical and biochemical study using antibodies to intermediate filament cytoskeletal proteins. Histopathology 11:487–499
Nouwen EJ, Pollet DE, Schelstraete JB, Eerdekens MW, Hansch C (1985) Human placental alkaline phosphatase in benign and malignant ovarian neoplasia. Cancer Res 45:892–902
Scully RE (1979) Tumors of the ovary and maldeveloped gonads. Washington D.C. Armed Forces Institute of Pathology
Scully RE (1984) Immunohistochemistry of ovarian tumors. In: Jose Russo (ed) Immuno-cytochemistry in tumor diagnosis. Martinus Nijhoff Publishing, Boston, Dordrecht, Lancaster, pp 293–321
Sloane JP, Hughes F, Ormerod MG (1983) An assessment of the value of epithelial membrane antigen and other epithelial markers in solving diagnostic problems in tumor histopathology. Histochem J 15:645–654
Tetu B, Ordonez NG, Silva EG (1986) Sertoli-Leydig cell tumor of the ovary with alpha-fetoprotein production. Arch Pathol Lab Med 110:65–68
Young RH, Scully RE (1982) Ovarian sexcord-stromal tumors: recent progress. Int J Gynecol Pathol 1:101–123
Young RH, Perez-Atayde AR, Scully RE (1984) Ovarian Sertoli-Leydig cell tumor with retiform and heterologous components. Am J Surg Pathol 8:709–718
About this article
Cite this article
Chadha, S., van der Kwast, T.H. Immunohistochemistry of ovarian granulosa cell tumours. Vichows Archiv A Pathol Anat 414, 439–445 (1989). https://doi.org/10.1007/BF00718628
- Granulosa cell tumour
- Intermediate filament proteins
- Tumour markers
- Ovarian carcinoma