Cell-free extracts of mycelia and sclerotia ofSclerotinia sclerotiorum (Lib.) D By. grown on synthetic liquid medium with various carbon sources containedd-mannitol-1-phosphate: NAD oxidoreductase (EC 18.104.22.168) and weakd-mannitol: NADP oxidoreductase (EC 22.214.171.124) activity. The difference in specific activity of the two enzymes coupled with the presence ofd-mannitol-1-phosphatase activity indicates the major pathway for mannitol synthesis.
Both enzymes were present in mycelia and sclerotia at all times, but highest activity occurred during early stages of growth. The optimum pH for reactions 1,2 and 3 was 7.5, 10.5 and 6.5 respectively. The oxidoreductase was quite specific in substrate requirements. Both enzymes were inhibited by sulfhydryl reagents and the phosphatase was activated by Mg2+.
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Wang, S.C., Le Tourneau, D. Mannitol biosynthesis inSclerotinia sclerotiorum . Archiv. Mikrobiol. 81, 91–99 (1972). https://doi.org/10.1007/BF00715026
- Carbon Source
- High Activity
- Liquid Medium