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Photosystem II quantum yield as a measure of radical scavengers in chilling injury in cucumber fruits and bell peppers

A static, dynamic and statistical model

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Abstract

Cucumber fruits (Cucumis sativus L. cv. Jessica) and green bell peppers (Capsicum annuum L. cvs. Lokas and Medeo) were stored at different temperatures ranging from 2 to 12°C. After three different storage periods, fruits from each temperature were transferred to 20°C for 7 d to allow for the development of visual symptoms of chilling injury (CI). During storage, the photochemical quantum yield of photosystem II (PSII) in peel tissue adapted to darkness, was calculated from measurements of pulse-amplitude-modulated chlorophyll fluorescence. The decrease in PSII quantum yield during storage at low temperatures in darkness can be described as a temperature-dependent inhibition of an enzyme according to Arrhenius, assuming a negative activation energy. By comparison with the radical-scavenger measurements of Hariyadi and Parkin (1991, Postharvest Biol. Techn. 1, 33–45) it is postulated that the time and temperature dependence of the quantum yield parallels the diminution of radical-scavenging activity at lower temperatures in cucumber and capsicum fruits. This is combined with an equation for the process of radical scavenging itself and an equation for the auto-catalytic radical-producing lipid peroxidation reaction. These three basic processes lead to both a static and a dynamic model for the occurrence of chilling injury in low-temperature-sensitive plant tissue. A statistical fit of the measured data using the static model leads to the estimates of the different activation energies and reaction rates with a high degree of accuracy. The estimated values are in accord with what one would expect on the basis of knowledge of the processes leading to chilling injury, and directly point to meaningful physico-chemical parameters.

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Abbreviations

C:

Symmetry point of logistic curve

CI:

intensity of chilling injury

e:

base of the natural logarithm system

E:

activation energy

Eff:

efficiency

Fv/Fm :

quantum yield of PSII

k:

reaction rate constant

R:

amount of free radicals

S:

amount of substrate for chilling injury (double bonds in fatty-acid chains)

t:

time

T:

temperature

Z:

amount of radical-scavenging enzyme Indices

CI:

chilling injury

d:

denaturation

i:

any index

max:

maximum value

min:

minimum value

r:

radical scavenging

ref:

reference (temperature)

s:

substrate

0:

initial amount

References

  1. Abe, K. (1990) Ultrastructural changes during chilling stress. In: Chilling injury of horticultural crops, 71–84, Wang, C.Y., ed. CRC Press, Boca Raton, Florida

  2. Asada, K., Takahashi, M. (1987) Production and scavenging of active oxygen in photosynthesis. In: Photoinhibition. Topics in photosynthesis, vol. 9, pp. 227–288, Kyle, D.J., Osmond, C.B., Arntzen, C.J., eds. Elsevier, Amsterdam

  3. Biesheuvel, A., van Kruistum, G. (1992) Lage temperatuur-bederf aanpakken met kou. Groente en Fruit50, 6–7

  4. Björkman, O., Demmig, B. (1987) Photon yield of O2 evolution and chlorophyll fluorescence characteristics at 77 K among vascular plants of diverse origins. Planta170, 489–504

  5. Chrominski, A., Khan, M.A., Weber, D.J., Smith, B.N. (1986) Ethylene and ethane production in response to salinity stress. Plant Cell Environ. 9, 687–691

  6. Elstner, E.F., Konze, J.R. (1976) Effect of point freezing on ethylene and ethane production by sugar beet leaf disks. Nature263, 351–352

  7. Feng, Y., Li, X., Boersma, L. (1990) The Arrhenius equation as a model for explaining plant responses to temperature and water stresses. Ann. Bot. 66, 237–244

  8. Foyer, Ch.H., Lelandais, M., Edwards, E.A., Mullineaux, Ph.M. (1991) The role of ascorbate in plants, interactions with photosynthesis, and regulatory significance. In: Active oxygen/oxidative stress and plant metabolism, pp. 131–144, Pell, E.J., Steffen, K.L., eds. American Society Plant Physiology, Rockville, Maryland

  9. Gounaris, K., Selkirk, M.E. (1992) Oxidative damage in photosystem 2. Robert Hill Symposium on Photosynthesis, Book of abstracts, p. 51, Barber, J., ed. London, UK

  10. Graham, D., Patterson, B.D. (1982) Responses of plants to low, non-freezing temperatures: proteins, metabolism and acclimation. Annu. Rev. Plant Physiol. 33, 347–372

  11. Halliwell, B. (1987) Oxidative damage, lipid peroxidation and antioxidant protection in chloroplasts. Chem. Phys. Lipids44, 327–340

  12. Hariyadi, P., Parkin, K.L. (1991) Chilling-induced Oxidative stress in cucumber fruits. Postharvest Biol. Techn. 1, 33–45

  13. Johnson, I.R., Thornley, J.H.M. (1985) Temperature dependence of plant and crop processes. Ann. Bot. 55, 1–24

  14. Kuo, S-J., Parkin, K.L. (1989) Chilling injury in cucumbers (Cucumis sativus L.) associated with lipid peroxidation as measured by ethane evolution. J. Food Sci. 54, 1488–1491

  15. Minorsky, P.V. (1985) An heuristic hypothesis of chilling injury in plants: a role for calcium as the primary physiological transducer of injury. Plant Cell Environ. 8, 75–94

  16. Nguyen, X.V., Mazliak, P. (1990) Chilling injury induction is accompanied by galactolipid degradation in tomato pericarp. Plant Physiol. Biochem. 28, 283–291

  17. Parkin, K.L., Kuo, S-J. (1989) Chilling-induced lipid degradation in cucumber (Cucumis sativus L. cv Hybrid C) fruit. Plant Physiol. 90, 1049–1056

  18. Saczynska, V., Gemel, J., Kaniuga, Z. (1993) Photosynthetic apparatus of chilling-sensitive plants. 28. Chilling susceptibility of Cucumis sativus species. Phytochemistry33, 61–67

  19. Saltveit, M.E. Jr., Cabrera, R.M. (1987) Tomato fruit temperature before chilling influences ripening after chilling. HortScience22, 452–454

  20. Schöner, S., Krause, G.H. (1990) Protective systems against active oxygen species in spinach: response to cold acclimation in excess light. Planta180, 383–389

  21. Senaratna, T., McKersie, B.D. (1986) Loss of desiccation tolerance during seed germination: a free radical mechanism of injury. In: Membranes, metabolism and dry organisms, pp. 85–101, Leopold, A.C., eds. Cornell University Press, Ithaca, NY

  22. Sierenberg, R.W., de Gans, O.B. (1987) Personal Prosim user guide. Sierenberg en de Gans bv, Waddinxveen, The Netherlands

  23. Sierenberg, R.W., de Gans, O.B. (1988) Introduction to Personal Prosim. Q-Passport no4, pp. 7–16

  24. Sommersalo, S., Krause, G.H. (1989) Photoinhibition at chilling temperature: fluorescence characteristics of unhardened and cold-acclimated spinach leaves. Planta177, 409–416

  25. Terashima, I., Kashino, Y., Katoh, S. (1991a) Exposure of leaves of Cucumis sativus L. to low temperatures in the light causes uncoupling of thylakoids I. Studies with isolated thylakoids. Plant Cell Physiol. 32, 1267–1274

  26. Terashima, I., Sonoike, K., Kawazu, T., Katoh, S. (1991b) Exposure of leaves of Cucumis sativus L. to low temperatures in the light causes uncoupling of thylakoids II. Non-destructive measurements with intact leaves. Plant Cell Physiol. 32, 1275–1283

  27. Van Kooten, O., Snel, J.F.H. (1990) The use of fluorescence nomenclature in plant stress physiology. Photosynth. Res. 25, 147–150

  28. Van Kooten, O., Mensink, M.G.J., Otma, E.C., Van Schaik, A.C.R., Schouten, S.P. (1992) Chilling damage of dark stored cucumbers (Cucumis sativus 1.) affects the maximum quantum yield of photosystem 2. In: Progress in photosynthesis research, vol. IV, pp.161–164, Murata, N., ed. Kluwer Acad. Publ. Dordrecht

  29. Wise, R.R., Naylor, A.W. (1987a) Chilling-enhanced photooxidation: the peroxidative destruction of lipids during chilling injury to photosynthesis and ultrastructure. Plant Physiol. 83, 272–277

  30. Wise, R.R., Naylor, A.W. (1987b) Chilling-enhanced photooxidation: evidence for the role of singlet oxygen and superoxide in the breakdown of pigments and endogenous antioxidants. Plant Physiol. 83, 278–282

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This study was conducted in the framework of a research program on fruits and vegetables, partly financed by the Dutch Commodity Board for Vegetables and Fruits.

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Tijskens, L.M.M., Otma, E.C. & van Kooten, O. Photosystem II quantum yield as a measure of radical scavengers in chilling injury in cucumber fruits and bell peppers. Planta 194, 478–486 (1994). https://doi.org/10.1007/BF00714459

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Key words

  • Chilling injury
  • Chlorophyll fluorescence
  • Mathematical model
  • Radical scavenging