Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Influence of spinal cord stimulation upon myosin light chain and tropomyosin subunit expression in a fast muscle (posterior latissimus dorsi) of the chick embryo

  • 20 Accesses

  • 14 Citations

Summary

Latissimus dorsi muscles of the chick consist of a slow (ALD) and a fast (PLD) muscle. The influence of chronic spinal cord stimulation in the chick embryo upon the expression of myosin light chains and tropomyosin subunits was investigated. Early in development the two muscles exhibited the same ratio of α- and β-tropomyosin subunits. Later, in the slow muscle the ratio β:α decreased and in chicken the amounts of the two components were about the same. In the fast muscle, the α-subunit increased and reached 66% in young chicken. In the early stages of embryonic development, both muscles accumulated slow and fast light chains. However, in ALD the amount of slow light chains was greater than that of fast light chains and the reverse was observed in PLD muscle. Later during development, the slow components decreased in PLD while the fast components increased; the reverse was observed in ALD muscle. The fast myosin LC3f has been detected in 18-day-old embryonic PLD.

Chronic spinal cord stimulation at a low rhytm was performed from day 10 of embryonic development to day 15 or 16. In both muscles from spinal cord-stimulated embryos, the β-tropomyosin subunit was lower than in control embryos. In ALD, the pattern of light chains was unaffected by chronic stimulation while in PLD muscle the slow and fast components were modified. In particular the ratio LCs:LCf was increased in spinal cord-stimulated embryos with regard to controls.

This is a preview of subscription content, log in to check access.

References

  1. ASHMORE, C. R. & DOERR, L. (1971) Postnatal development of fiber types in normal and dystrophic skeletal muscle of the chick.Expl Neurol. 30, 431–46.

  2. BAGUST, J., LEWIS, D. M. & WESTERMAN, R. A. (1981) Motor units in cross-reinnervated fast and slow twitch muscle of the cat.J. Physiol. 313, 223–35.

  3. BÁRÁNY, M. & CLOSE, R. I. (1971) The transformation of myosin in cross-innervated rat muscles.J. Physiool. 213, 455–74.

  4. BENFIELD, P. A., LOWEY, S., LE BLANC, D. D. & WALLER, G. S. (1983) Myosin isozymes in avian skeletal muscles. II. Fractionation of myosin isozymes froma dult and embryonic chickenpecgoralis muscle by immunoaffinity chromatography.J. Musc. Res. Cell Motility 4, 717–38.

  5. BENNETT, M. R. & PETTIGREW, A. G. (1974) The transformation of synapses in reinneryvated and cross-reinnervated striated muscle during development.J. Physiol. 241, 547–73.

  6. BENNETT, M. R., PETTIGREW, A. G. & TAYLOR, R. S. (1973) The formation of synapses in reinnervated and cross-reinnervated adult avian muscle.J. Physiol. 230, 331–57.

  7. BROWN, W. e., SALMONS, S. & WHALEN, R. G. (1983) The sequential replacement of myosin subunit isoforms during muscle type transformation induced by long term electrical stimulation.J. biol. Chem. 258, 14686–92.

  8. CROW, M. T., OLSON, P. S., CONLON, S. B. & STOCKDALE, F. E. (1983a) Myosin light chain expressionin the developing avian hindlimb.Limb Dev. Regen., part B, 417–28.

  9. CROW, M. T., OLSON, P. S. & STOCKDALE, F. E. (1983b) Myosin light-chain expression during avian muscle development.J. Cell Biol. 96, 736–44.

  10. GAUTHIER, G. F., BURKE, R. E., LOWEY, S. & HOBBS, A. W (1983) Myosin isozymes in normal and cross-reinnervated cat skeletal muscle fibers.J. Cell Biol. 97, 756–71.

  11. GAUTHIER, G. F., LOWEY, S., BENFIELD, P. A. & HOBBS, A. W. (1982) Distribution and properties of myosin isozymes in developing avian adn mammalian skeletal muscle fibers.J. Cell Biol. 92, 471–84.

  12. GAUTHIER, G. F., LOWEY, S. & HOBBS, A. W. (1978) Fast and slow myosin in developing muscle fibres.Nature 274, 25–9.

  13. HNÍK, P., JIRMANOVÁ, I., VYKLICKÝ, L. & ZELENÁ, J. (1967) Fast and slow muscles of the chick after nerve cross-union.J. Physiol. 193, 309–25

  14. HOH, J. F. Y. (1975) Neural regulation of mammalian fast and slow muscle myosins: an electrophoretic analysis.Biochemistry 14, 724–7.

  15. HOH, J. F. Y. (1979) Developmental changes in chicken skeletalm yosin isoenzymes.FEBS Lett. 98, 267–70.

  16. HOH, J. F. Y., KWAN, B. T. S., DUNLOP, C. & KIM, B. H. (1980) Effects of nerve cross-union and cordotomy on myosin isoenzymes in fast-twitch and slow-twitch muscles of the rat. InPlasticity of Muscle (edited by PETTE, D.), pp. 339–52. Berlin, New York: de Gruyter.

  17. KELLER, L. R. & EMERSON, C. P., JR (1980) Synthesis of adult myosin light chains by embryonic muscle cultures.Proc. natn. Acad. Sci. U.S.A. 77, 1020–4.

  18. KOENIG, J. (1967) Structure et distribution des plaques motrices d'un muscle lent réinnervé expérimentalement par le nerf moteur d'un muscle rapide.C.R. Soc. Biol. 161, 1276–79.

  19. LØMO, T. R., WESTGAARD, R. H. & DAHL, H. A. (1974) Contractile properties of muscle: control by pattern of muscle activity in the rat.Proc. R. Soc. Ser. B. 187, 99–103.

  20. LOWEY, S., BENFIELD, P. A., LE BLANC, D. D. & WALLER, G. S. (1983) Myosin isozymes in avian skeletal muscles. I. Sequential expression of myosin isozymes in developing chicken pectoralis muscles.J. Musc. Res. Cell Motility 4, 695–716.

  21. LOWRY, O. H., ROSEBROUGH, N. J. FARR, A. L. & RANDALL, R. J. (1951) Protein measurement with the Folin phenol reagent.J. biol. Chem. 193, 265–75.

  22. MABUCHI, K., SZVETKO, D., PINTÉR, K. & SRÉTER, K., & SRÉTER, F. A. (1982) Type IIB to IIA fiber transformation in intermittently stimulated rabbit muscles.Am. J. Physiol. 242, C373–81.

  23. MATSUDA, R., BANDMAN, E. & STROHMAN, R. C. (1983) Regional differences in the expression of myosin light chains and tropomyosin subunits during development of chicken breast muscle.Devl Biol. 95, 484–91.

  24. MOMMAERTS, W. F. H. M., SERAYDARIAN, K., SUH, M., KEAN, C. J. C. & BULLER, A. J. (1977) The conversion of some biochemical properties of mammalian skeletal muscles following cross-reinnervation.Expl Neurol. 55, 637–53.

  25. MONTARRAS, D., FISZMAN, M. Y. & GROS, F. (1982) Changes in tropomyosin during development of chick embryonic skeletal musclesin vivo and during differentiation of chick muscle cellsin vitro.J. biol. Chem. 251, 545–8.

  26. O'FARRELL, P. H. (1975) High resolution two-dimensional electrophoresis of proteins.J. biol. Chem. 250, 4007–21.

  27. PETTE, D., VRBOVÁ, G. & WHALEN, R. G. (1979) Independent development of contractile properties and myosin light chains in embryonic chick fast and slow muscle.Pflügers Arch. 378, 251–7.

  28. PETTE, D. & SCHNEZ, U. (1977) Coexistence of fast and slow type myosin light chains in single muscle fibres during transformation as induced by long term stimulation.FEBS Lett. 83, 128–30.

  29. RENAUD, D., GARDAHAUT, M.-F., ROUAUD, T. & LE DOUARIN, G. H. (1983) Influence of chronic spinal cord stimulation upon differentiation of β muscle fibers in a fast muscle (Posterior Latissimus dorsi) of the chick embryo.Expl Neurol. 80, 157–66.

  30. RENAUD, D., LE DOUARIN, G. H. & KHASKIYE, A. (1978) Spinal cord stimulation in chick embryo: effects on development of the posterior latissimus dorsi muscle and neuromuscular junctions.Expl Neurol. 60, 189–200.

  31. ROY, R. K., MABUCHI, K., SARKAR, S., MIS, C. & SRÉTER, F. A. (1979a) Changes in tropomyosin subunit pattern inc hronic electrically stimulated rabbit fast muscles.Biochem. biophys. Res. Commun. 89, 181–7.

  32. ROY, R. K., SRÉTER, F. A. & SARKAR, S. (1979b) Chanbes in tropomyosin subunits and myosin light chains during development of chicken and rabbit striated muscles.Devl Biol. 69, 15–30.

  33. RUBINSTEIN, N. A. & KELLY, A. M. (1978) Myogenic and neurogenic contributions to the development of fast and slow twich muscles in rat.Devl Biol. 62, 473–85.

  34. RUBINSTEIN, N., MABUCHI, K., PEPE, F., SALMONS, S., GERGELY, J. & SRÉTER, F. (1978) Use of type-specific antimyosins to demonstrate the transformation of individual fibers in chronically stimulated rabbit fast muscles.J. Cell Biol. 79, 252–61.

  35. RUBINSTEIN, N. A., PEPE, F. A. & HOLTZER, H. (1977) Myosin types during the development of embryonic chicken fast and slow muscles.Proc. natn. Acad. Sci. U.S.A. 74, 4524–7.

  36. SEEDORF, K., SEEDORF, U. & SETTE, D. (1983) Coordinate expression of alkali and DTNB myosin light chains during transformation of rabbit fast muscle by chronic stimulation.FEBS Lett. 158, 321–4.

  37. SRÉTER, F. A. & GERGELY, J. (1973) Synthesis by fast muscle of myosin light chains characteristic of slow muscle in response to long-term stimulation.Nature 241, 17–8.

  38. SRÉTER, F. A., GERGELY, J. & LUFF, A. L. (1974) the effect of cross-reinnervation on the synthesis of myosin light chains.Biochem. biophys. Res. Commun. 56, 84–9.

  39. SRÉTER, F. A., PINTÉR, K., JOLESZ, F., & MABUCHI, K. (1982) Fast to slow transformation of fast muscles in response to long-term phasic stimulation.Expl Neurol. 75, 95–102.

  40. SRIHARI, T., SEEDORF, U. & PETTE, D. (1981) Ipsi- and contralateral changes in rabbit soleus myosins by cross-reinnervation.Pflügers Arch. 390, 246–9.

  41. STOCKDALE, F. E., RAMAN, N. & BADEN, H. (1981) Myosin light chains and the developmental origin of fast muscle.Proc. natn. Acad. Sci. U.S.A. 78, 931–5.

  42. SYROVÝ, I. & ZELENÁ, J. (1975) The onset and progress of transformation of avian slow into fast muscles under neural influence.Pflügers Arch. 360, 121–34.

  43. TOUTANT, M., TOUTANT, J. P., MONTARRAS, D. & FISZMAN, M. Y. (1983) Potential phasic and tonic muscles express a common set of fast and slow myosin light chains and fast tropomyosin during early development of chick embryo.Biochimie 65, 637–42.

  44. WEEDS, A. G., TRENTHAM, D. R., KEAN, C. J. C. & BULLER, A. J. (1974) Myosin from cross-reinnervated cat muscles.Nature 247, 135–9.

Download references

Author information

Correspondence to M. F. Gardahaut.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Gardahaut, M.F., Rouaud, T., Renaud, D. et al. Influence of spinal cord stimulation upon myosin light chain and tropomyosin subunit expression in a fast muscle (posterior latissimus dorsi) of the chick embryo. J Muscle Res Cell Motil 6, 769–781 (1985). https://doi.org/10.1007/BF00712241

Download citation

Keywords

  • Spinal Cord
  • Light Chain
  • Embryonic Development
  • Chick Embryo
  • Myosin Light Chain