F-actin filaments are disrupted by the action of cytochalasin and glutaraldehyde. Muscle tropomyosin which is able to polymerize can protect F-actin against fragmentation caused by these two agents. This protective effect does not occur with nonpolymerizable, brain or carboxypeptidase A-treated skeletal muscle tropomyosins. The protection of F-actin against the action of cytochalasin and glutaraldehyde takes place under conditions where the F-actin filaments are saturated with tropomyosin, that is, at a molar ratio of tropomyosin to actin of 1 : 7. It is suggested that nonpolymerizable tropomyosin lacks the protective ability because its binding to F-actin is considerably weaker than the polymerizable tropomyosin and does not saturate all of the binding sites on F-actin.
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BAILEY, K. (1948) Tropomyosin a new asymmetric protein component of the muscle fibril.Biochem. J. 43, 271–9.
BRAY, D. (1979) Cytochalasin action.Nature 282, 671.
BRENNER, S. L. & KORN, E. D. (1979) Substoichiometric concentrations of cytochalasin D inhibit actin polymerization.J. biol. Chem. 254, 9982–5.
BRENNER, S. L. & KORN, E. D. (1980) The effects of cytochalasins on actin polymerization and actin ATPase provide insights into the mechanism of polymerization.J. biol. Chem. 255, 841–4.
COHEN, I. & COHEN, C. A. (1972) Tropomyosin-like protein from human platelets.J. molec. Biol. 68, 383–9.
CÔTÉ, G. P., LEWIS, W. G. & SMILLIE, L. B. (1978) Non-polymerizability of platelet tropomyosin and its NH2- and COOH-terminal sequences.FEBS Lett. 91, 237–41.
DĄBROWSKA, R., NOWAK, E. & DRABIKOWSKI, W. (1980a) Comparison of functional properties of non-muscle and muscle tropomyosin.Eur. J. Cell Biol. 22, 319.
DĄBROWSKA, R., NOWAK, E. & DRABIKOWSKI, W. (1980b) Comparison of some functional properties of non-polymerizable and polymerizable tropomyosin.J. Musc. Res. Cell Motility 1, 466.
DĄBROWSKA, R., SOSINSKI, J. & DRABIKOWSKI, W. (1980c) Comparative studies of various kinds of tropomyosin. InPlasticity of Muscle (edited by PETTE, D.), pp. 225–239. Berlin, New York: Walter de Gruyter.
DOBROWOLSKI, Z., BARYŁKO, B. & DRABIKOWSKI, W. (1980) Distribution of contractile proteins in brain.J. Musc. Res. Cell Motility 1, 487.
DRABIKOWSKI, W., DĄBROWSKA, R. & BARYŁKO, B. (1973) Properties of troponin and its constituents.Acta biochem. polon. 20, 181–99.
DRENCKHAHN, D. & GRÖSCHEL-STEWART, U. (1980) Localization of myosin, actin and tropomyosin in rat intestinal epithelium: immunohistochemical studies at the light and electron microscope levels.J. Cell Biol. 86, 475–82.
GADASI, H., OPLATKA, A., LAMED, R., HOCHBERG, A. & LOW, W. (1974) Possible uncoupling of the mechanochemical process in the actomyosin system crosslinking of F-actin.Biochim. biophys. Acta 333, 161–8.
GORNALL, A. G., BARDAWILL, C. J. & DAVID, M. M. (1949) Determination of serum proteins by means of the biuret reaction.J. biol. Chem. 177, 751–66.
GRUMET, M. & LIN, S. (1980) A platelet inhibitor protein with cytochalasin-like activity against actin polymerizationin vitro.Cell 21, 439–44.
HARTWIG, J. H. & STOSSEL, T. P. (1979) Cytochalasin B and structure of actin gels.J. molec. Biol. 134, 539–53.
HITCHCOCK-De GREGORI, S. E. (1980) Actin assembly.Nature 288, 437–8.
ISENBERG, G., AEBI, U. & POLLARD, T. D. (1980) An actin binding protein from Acanthamoeba regulates actin filament polymerization and interactions.Nature 288, 455–9.
KITAGAWA, S., DRABIKOWSKI, W. & GERGELY, J. (1968) Exchange and release of the bound nucleotide of F-actin.Archs Biochem. Biophys. 125, 707–14.
LAZARIDES, E. (1975) Two general classes of cytoplasmic actin filaments in tissue culture cells: the role of tropomyosin.J. Cell Biol. 65, 549–61.
LAZARIDES, E. (1977) Actin, α-actinin and tropomyosin interaction in the structural organization of actin filaments in nonmuscle cells.J. Supramolec. Struct. 5, 531–63.
LEHRER, S. S. (1972) The crosslinking of actin and tropomyosin by glutaraldehyde.Biochem. biophys. Res. Commun. 48, 967–76.
LIN, D. K., TOBIN, D., GRUMET, M. & LIN, S. (1980) Cytochalasins inhibit nuclei-induced actin polymerization by blocking filament elongation.J. Cell. Biol. 84, 455–60.
LÖW, I., DANCKER, P. & WIELAND, T. H. (1975) Stabilization of F-actin by phalloidin; reversal of the destabilizing effect of cytochalasin B.FEBS Lett. 54, 263–65.
LOWRY, O. H., ROSEBROUGH, R. J., FARR, A. R. & RANDALL, R. J. (1951) Protein measurements with the Folin reagent.J. biol. Chem. 193, 265–75.
MARUYAMA, K., HARTWIG, J. H. & STOSSEL, T. P. (1980) Cytochalasin B and the structure of actin gels. II. Further evidence for the splitting of F-actin by cytochalasin B.Biochim. biophys. Acta 626, 494–500.
MAUPIN-SZAMIER, P. & POLLARD, T. D. (1978) Actin filament destruction by osmium tetroxide.J. Cell Biol. 77, 837–52.
MIKAWA, T. (1979) ‘Freezing’ of Ca-regulated conformation of reconstituted thin filaments of skeletal muscle.Nature 278, 473.
OOSAWA, F., FUJIME, S., ISHIWATA, S. & MIHASHI, K. (1972) Dynamic property of F-actin and thin filament.Cold Spring Harb. Symp. quant. Biol. 23, 277–85.
PRÒCHNIEWICZ, E. (1979) Effect of crosslinking by glutaraldehyde on interaction of F-actin with heavy meromyosin.Biochim. biophys. Acta 579, 346–58.
SCHLOSS, J. A. & GOLDMAN, R. D. (1980) Microfilaments and tropomyosin of cultured mammalian cells: isolation and characterization.J. Cell Biol. 87, 633–42.
SPUDICH, J. A. (1972) Effect of cytochalasin B on actin filaments.Cold Spring Harb. Symp. quant. Biol. 37, 585–93.
STRZELECKA-GOŁASZEWSKA, H., JAKUBIAK, M. & DRABIKOWSKI, W. (1975) Changes in the state of actin during superprecipitation of actomyosin.Eur. J. Biochem. 55, 221–30.
UENO, H., TAWADA, Y. & OOI, T (1976) Properties of non-polymerizable tropomyosin obtained by carboxy-peptidase A digestion.J. Biochem. 80, 238–90.
WEBER, K. & OSBORN, M. (1969) The reliability of molecular weight determinations by dodecyl sulphate-polyacrylamide gel electrophoresis.J. biol. Chem. 244, 4406–10.
WEHLAND, J. & WEBER, K. (1980) Distribution of fluorescently labeled actin and tropomyosin after microinjection in living tissue culture cells as observed with TV image intensification.Expl Cell Res. 127, 397–408.
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Dabrowska, R., Próchniewicz, E. & Drabikowski, W. The effect of cytochalasin and glutaraldehyde on F-actin filaments containing muscle and non-muscle tropomyosin. J Muscle Res Cell Motil 4, 83–93 (1983). https://doi.org/10.1007/BF00711959
- Skeletal Muscle
- Protective Effect
- Protective Ability