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The effect of cytochalasin and glutaraldehyde on F-actin filaments containing muscle and non-muscle tropomyosin

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Summary

F-actin filaments are disrupted by the action of cytochalasin and glutaraldehyde. Muscle tropomyosin which is able to polymerize can protect F-actin against fragmentation caused by these two agents. This protective effect does not occur with nonpolymerizable, brain or carboxypeptidase A-treated skeletal muscle tropomyosins. The protection of F-actin against the action of cytochalasin and glutaraldehyde takes place under conditions where the F-actin filaments are saturated with tropomyosin, that is, at a molar ratio of tropomyosin to actin of 1 : 7. It is suggested that nonpolymerizable tropomyosin lacks the protective ability because its binding to F-actin is considerably weaker than the polymerizable tropomyosin and does not saturate all of the binding sites on F-actin.

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Dabrowska, R., Próchniewicz, E. & Drabikowski, W. The effect of cytochalasin and glutaraldehyde on F-actin filaments containing muscle and non-muscle tropomyosin. J Muscle Res Cell Motil 4, 83–93 (1983). https://doi.org/10.1007/BF00711959

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Keywords

  • Skeletal Muscle
  • Protective Effect
  • Glutaraldehyde
  • Cytochalasin
  • Protective Ability