The force-velocity (P–V) relation for single fibres isolated from the semitendinosus muscle of the frog was determined at preset times during the rise of tension and the plateau of isometric tetani. The controlled-velocity release method was used. Experiments were performed at a sarcomere length of about 2.25 µm.
Addition of dantrolene-sodium (DaNa) to Ringer's solution resulted in a large reduction of the peak twitch tension and of the speed of rise of twitch and tetanic tensions. The plateau tetanic tension was either unaffected or only slightly reduced by DaNa. In all fibres the speed of rise of isometric tension after a quick release imposed at the tetanus plateau was not affected by DaNa.
DaNa did not significantly affect the observed value ofV 0 and the calculated values of Hill's constantsV 0,P 0 * , a/P 0 * andb for andP–V relation determined at the tetanus plateau. In accordance with previous work, during the tetanus rise theP–V relation gradually attained its final characteristics, but there was a significant delay of the development of the isometric tension with respect to the development of theP–V relation. Treatment of the fibre with DaNa increased both the development time of theP–V relation and the level of isometric tension at which during the tetanus rise theP–V relation attained its final characteristics.
It is concluded that DaNa, which inhibits the release of Ca2+ from the sarcoplasmic reticulum, also depresses the rate of development of activation.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
CAMBRIDGE, E. W. & HAINES, J. (1959) A new versatile transducer system.J. Physiol. 149, 2–3P.
CECCHI, G., COLOMO, F. & LOMBARDI, V. (1976) A loudspeaker servo system for determination of mechanical characteristics of isolated muscle fibres.Boll. Soc. it. Biol. sper. 52, 733–6.
CECCHI, G., COLOMO, F. & LOMBARDI, V. (1978) Force-velocity relation in normal and nitrate-treated frog single muscle fibres during rise of tension in an isometric tetanus.J. Physiol. 285, 257–73.
CECCHI, G., COLOMO, F. & LOMBARDI, V. (1979a) A capacitance-gauge force transducer for isolated muscle fibres.J. Physiol. 292, 1–2P.
CECCHI, G., COLOMO, F. & PIAZZESI, G. (1980) Kinetics of the activation process in normal and dantrolene-treated frog single muscle fibres.Proceedings of the International Union of Physiological Sciences, Budapest, 1980, Vol. 14/1005, p. 361.
CECCHI, G., COLOMO, F. & LOMBARDI, V. (1981) Force-velocity relation in deuterium oxide-treated frog single muscle fibres during the rise of tension in an isometric tetanus.J. Physiol.,317, 207–21.
CECCHI, G., COLOMO, F., LOMBARDI, V. & PIAZZESI, G. (1979b) Development of activation and rise of tension in an isometric tetanus.Pflügers Arch. 381, 71–4.
EDMAN, K. A. P. (1979) The velocity of unloaded shortening and its relation to sarcomere length and isometric force in vertebrate muscle fibres.J. Physiol. 291, 143–59.
EDMAN, K. A. P. (1981) Deactivation of the contractile system induced by shortening of striated muscle. InThe Regulation of Muscle Contraction: Excitation-Contraction Coupling (edited by GRINNELL, A. D. and BRAZIER, M. A. B.), pp. 281–96.
EDMAN, K. A. P., MULIERI, L. A. & SCUBON-MULIERI, B. (1976) Non-hyperbolic force-velocity relationship in single muscle fibres.Acta physiol. scand. 98, 143–56.
ELLIS, K. O. & CARPENTER, J. F. (1972) Studies on the mechanism of action of dantrolene sodium.Arch. exp. Path. Pharm. 275, 83–94.
FORD, L. E., HUXLEY, A. F. & SIMMONS, R. M. (1977) Tension responses to sudden length change in stimulated frog muscle fibres near slack length.J. Physiol. 269, 441–515.
HAINAUT, K. & DESMEDT, J. E. (1974) Effect of dantrolene sodium on calcium movements in single muscle fibres.Nature 252, 728–30.
HILL, A. V. (1938) The heat of shortening and the dynamic constants of muscle.Proc. R. Soc. 126, 136–95.
HUXLEY, A. F. (1957) Muscle structure and theories of contraction.Prog. Biophys. biophys. Chem. 7, 255–318.
JEWELL, B. R. & WILKIE, D. R. (1958) An analysis of the mechanical components in frog's striated muscle.J. Physiol. 143, 515–40.
KATZ, B. (1939) The relation between velocity and force in muscular contraction.J. Physiol. 96, 45–64.
MOISESCU, D. G. (1976) Kinetics of reactions in calcium-activated skinned muscle fibres.Nature 262, 610–4.
TAYLOR, S. R., LOPEZ, J. R. & SHLEVIN, H. H. (1979) Calcium movements in relation to muscle contraction.Proc. West pharmacol. Soc. 22, 321–6.
VAN WINKLE, W. B. (1976) Calcium release from skeletal muscle sarcoplasmic reticulum: site of action of dantrolene sodium?Science 193, 1130–1.
About this article
Cite this article
Cecchi, G., Colomo, F. & Piazzesi, G. The development of the force-velocity relation in normal and dantrolene-treated frog single muscle fibres. J Muscle Res Cell Motil 4, 395–404 (1983). https://doi.org/10.1007/BF00711946
- Sarcoplasmic Reticulum
- Final Characteristic
- Versus Relation
- Sarcomere Length