Advertisement

Cellular and Molecular Neurobiology

, Volume 5, Issue 3, pp 285–296 | Cite as

Choline fluxes in synaptosomal membrane vesicles

  • H. Breer
  • M. Knipper
Article

Summary

  1. 1.

    Synaptic plasma membrane vesicles isolated from the highly cholinergic nervous tissue of insects were used to study the translocation of choline across the membrane via a high-affinity carrier-mediated mechanism energized by ion gradients as the sole driving force.

     
  2. 2.

    The uphill movement of choline, energized mainly by the Na+ gradient, attained levels of choline severalfold the final equilibrium value at the peak of the overshoot.

     
  3. 3.

    Efflux of choline required the presence of internal sodium ions and was promoted by external choline if Na+ was present. External choline inhibited choline efflux in the absence of sodium.

     
  4. 4.

    It is concluded that the efflux of choline is in many aspects symmetrical with its uptake.

     

Key words

choline efflux carrier ion gradients transactivation insect 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Barker, L. A., and Mittag, T. W. (1974). Comparative studies of substrate and inhibitors of choline transport and choline acetyltransferase.J. Pharmacol. Exp. Ther. 19286–94.Google Scholar
  2. Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microquantities of protein utilizing the principle of protein-dye binding.Anal. Biochem. 65248–254.Google Scholar
  3. Breer, H. (1981). Characterization of synaptosomes from the central nervous system of insects.Neurochem. Int. 3155–163.Google Scholar
  4. Breer, H. (1982). Uptake of [N-Me-3H]choline by synaptosomes from the central nervous system ofLocusta migratoria.J. Neurobiol. 13107–117.Google Scholar
  5. Breer, H. (1983). Choline transport by synaptosomal membrane vesicles isolated from the insect nervous tissue.FEBS Lett. 153345–348.Google Scholar
  6. Breer, H., and Lueken, W. (1983). Transport of choline by membrane vesicles prepared from synaptosomes of insect nervous tissue.Neurochem. Int. 5713–720.Google Scholar
  7. Breer, H., and Knipper, M. (1984). Characterisation of acetylcholine release from insect synaptosomes.Insect Biochem. 14337–344.Google Scholar
  8. Breer, H., and Knipper, M. (1985). Effects of neurotoxins on the high affinity translocation of choline in synaptosomal membrane vesicles from insects.Comp. Biochem. Physiol. 81C219–222.Google Scholar
  9. Crane, R. K. (1977). The gradient hypothesis and other models of carrier-mediated active transport.Rev. Physiol. Biochem. Pharmacol. 78100–150.Google Scholar
  10. Grinstein, S., and Cohen, S. (1983). Measurement of sidedness of isolated plasma-membrane vesicles: Quantitation of actin exposure by DNase I inactivation.Anal. Biochem. 130151–157.Google Scholar
  11. Heinz, E. (1977). Coupled transport of metabolites.Annu. Rev. Physiol. 2921–58.Google Scholar
  12. Jope, R. S. (1979). High affinity choline transport and acetylCoA production in brain and their roles in ACh synthesis.Brain Res. Rev. 1313–344.Google Scholar
  13. Kanner, B. I. (1978). Active transport ofγ-aminobutyrate by membrane vesicles isolated from rat brain.Biochemistry 171201–1211.Google Scholar
  14. Kanner, B. I. (1983). Bioenergetics of neurotransmitter transport.Biochim. Biophys. Acta 726293–316.Google Scholar
  15. Kanner, B. I., and Kifer, L. (1981). Efflux ofγ-aminobutyric acid by synaptic plasma membrane vesicles isolated from rat brain.Biochemistry 203354–3358.Google Scholar
  16. Kanner, B. I., and Sharon, I. (1978). Active transport of L-glutamate by membrane vesicles isolated from rat brain.Biochemistry 173949–3953.Google Scholar
  17. Krupka, R. M., and Deves, R. (1981). An experimental test for cyclic versus linear transport models.J. Biol. Chem. 2565410–5416.Google Scholar
  18. Kuhar, M. J., and Murrin, L. C. (1978). Sodium-dependent high affinity choline uptake.J. Neurochem. 3015–21.Google Scholar
  19. Marchbanks, R. M., and Wonnacott, S. (1979). Relationship of choline uptake to acetylcholine synthesis and release. InProgress in Brain Research, Vol. 49. The Cholinergic Synapse (S. Tucek, Ed.), Elsevier, Amsterdam, pp. 77–87.Google Scholar
  20. Marchbanks, R. M., Wonnacott, S., and Rubio, M. A. (1981). The effect of acetylcholine release on choline fluxes in isolated synaptic terminals.J. Neurochem. 36379–393.Google Scholar
  21. Mayor, F., Marvizòn, J. G., Aragon, M. C., Gimenez, C., and Valdivicso, F. (1981). Glycine transport into plasma membrane vesicles derived from rat brain synaptosomes.Biochem. J. 198535–541.Google Scholar
  22. Meyer, E. M., and Cooper, J. R. (1983). High affinity choline uptake and calcium-dependent release of ACh from proteoliposomes derived from rat cortical synaptosomes.J. Neurosci. 3987–994.Google Scholar
  23. Meyer, E. M., Engel, D. A., and Cooper, J. R. (1982). Acetylation and phosphorylation of choline following high and low affinity uptake by rat cortical synaptosomes.Neurochem. Res. 7749–759.Google Scholar
  24. Mitchell, P. (1967). Translocation through natural membranes.Adv. Enzymol. 2933–87.Google Scholar
  25. Nelson, M. T., and Blaustein, M. P. (1982). GABA efflux from synaptosomes: Effects of membrane potential, and external GABA and cations.J. Membr. Biol. 69213–223.Google Scholar
  26. Roskoski, R., Rauch, N., and Roskoski, L. M. (1981). Glutamate, aspartate, andγ-aminobutyrate transport by membrane vesicles prepared from rat brain.Arch. Biochem. Biophys. 207407–415.Google Scholar
  27. Rudnik, G. (1977). Active transport of 5-hydroxytryptamine by plasma membrane vesicles isolated from human blood platelets.J. Biol. Chem. 2522170–2174.Google Scholar

Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • H. Breer
    • 1
  • M. Knipper
    • 1
  1. 1.Department of ZoophysiologyUniversity OsnabrückOsnabrückWest Germany

Personalised recommendations