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Intermediate-filament expression in thyroid gland carcinomas

Summary

Paraffin-embedded specimens of 200 primary thyroid carcinomas were examined immunohistologically for the expression of intermediate-filament (IF) protein of the cytokeratin, vimentin and neurofilament type. In 36 cases, snap-frozen tissue was available, and double label immunofluorescence microscopy was performed in 23 of them.

Cytokeratin reactivity was found in all cells of all follicular, papillary and medullary carcinoma cases examined. Using a monoclonal vimentin antibody, positive staining was found in many, though not all cells of the papillary tumours and in approximately 50% of the follicular and the medullary carcinomas. Among anaplastic carcinomas, some tumours were positive for cytokeratins, with or without coexpression of vimentin. Neurofilaments could only be demonstrated in approximately 13% of medullary tumours which in general also exhibited vimentin positivity.

The differences of IF expression in follicle and C-cell thyroid carcinomas and the broad variation of cytokeratin and vimentin immunoreactivity among anaplastic tumours of this organ is discussed in relation to the possible intrinsic heterogeneity of these tumours and the diagnostic value of these marker.

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References

  1. Altmannsberger M, Osborn M, Schäfer H, Schauer A, Weber K (1984) Distinction of nephroblastomas from other childhood tumours using antibodies to intermediate filaments. Virchows Arch [Cell Pathol] 45:113–124

  2. Altmannsberger M, Dralle H, Weber K, Osborn M, Droese M (1986) Intermediate filaments in cytological specimens of thyroid tumours. Diagn Cytopathol (in press)

  3. Blobel GA, Gould VE, Moll R, Lee I, Huszar M, Geiger B, Franke WW (1985a) Coexpression of neuroendocrine markers and epithelial cytoskeletal proteins in bronchopulmonary neoplasms. Lab Invest 52:39–51

  4. Blobel GA, Moll R, Franke WW, Kayser KW, Gould VE (1985b) The intermediate filament cytoskeleton of malignant mesotheliomas and its diagnostic significance. Am J Pathol 121:235–247

  5. Carcangiu ML, Steeper T, Zampi G, Rosai J (1985) Anaplastic thyroid carcinoma. A study of 70 cases. Am J Clin Pathol 83:35–158

  6. Caselitz J, Osborn M, Seifert G, Weber K (1981) Intermediate-sized filament proteins (prekeratin, vimentin, desmin) in the normal parotid gland and parotid gland tumours. Immunofluorescence study. Virchows Arch [Pathol Anat] 393:273–286

  7. Caselitz J, Becker J, Seifert G, Weber K, Osborn M (1984) Coexpression of keratin and vimentin filaments in adenoid cystic carcinomas of salivary glands. Virchows Arch [Pathol Anat] 403:337–344

  8. Czernobilsky B, Moll R, Levy R, Franke WW (1985) Co-expression of cytokeratin and vimentin filaments in mesothelial, granulosa and rete ovarii cells of the human ovary. Eur J Cell Biol 37:175–190

  9. Denk H, Franke WW (1982) Cytoskeletal filament. In: Arias IM, Popper H, Schachter D, Shafriz DA (eds) The liver, biology and pathobiology. Raven Press, New York, pp 55–71

  10. Denk H, Weybora W, Ratschek M, Sohar R, Franke WW (1985) Distribution of vimentin, cytokeratins, and desmosomal-plaque proteins in human nephroblastoma as revealed by specific antibodies: co-existence of cell groups of different degrees of epithelial differentiation. Differentiation 29:88–97

  11. Droese M, Altmannsberg M, Dralle H (1984) Verteilung der Intermediärfilamente in Schilddrüsencarcinomen. Verh Dtsch Ges Pathol 68:498

  12. Dulbecco R, Allen R, Okada S, Bowman M (1983) Functional changes of intermediate filaments in fibroblastic cells revealed by a monoclonal antibody. Proc Natl Acad Sci (USA) 80:1915–1918

  13. Franke WW, Schmid E, Osborn M, Weber K (1978) Different intermediate-sized filaments distinguished by immunofluorescence microscopy. Proc Natl Acad Sci (USA) 76:5034–5038

  14. Franke WW, Schmid E, Breitkreutz D, Lüder M, Boukamp P, Fusenig NE, Osborn M, Weber K (1979a) Simultaneous expression of two different types of intermediate-sized filaments in mouse keratinocytes proliferating in vitro. Differentiation 14:35–50

  15. Franke WW, Appelhans B, Schmid E, Freudenstein C, Osborn lM, Weber K (1979b) Identification and characterization Of epithelial cells in mammalian tissues by immunofluorescence microscopy using antibodies to prekeratin. Differentiation 15:7–25

  16. Franke WW, Schmid E, Winter S, Osborn M, Weber K (1979c) Widespread occurrence of intermediate-sized filaments of the vimentin-type in cultured cells from diverse vertebrates. Exp Cell Res 123:25–46

  17. Franke WW, Schmid E, Freudenstein C, Appelhans B, Osborn M, Weber K, Keenan TW (1980) Intermediate-sized filaments of the prekeratin type in myoepithelial cells. J Cell Biol 84:633–654

  18. Franke WW, Grund C, Kuhn C, Lehto V-P, Virtanen I (1984) Transient changes of organization of vimentin filaments during mitosis as demonstrated by a monoclonal antibody. Exp Cell Res 154:567–580

  19. Gabbiani G, Kapanci Y, Barazzone P, Franke WW (1981) Immunochemical identification of intermediate-sized filaments in human neoplastic cells. A diagnostic aid for the surgical pathologist. Am J Pathol 104:206–216

  20. Gigi O, Geiger B, Eshhar Z, Moll R, Schmid E, Winter S, Schiller DL, Franke WW (1982) Detection of a cytokeratin determinant common to diverse epithelial cells by a broadly cross-reacting monoclonal antibody. EMBO J 1:1429–1437

  21. Gould VE (1985) The coexpression of distinct classes of intermediate filaments in human neoplasms. Arch Pathol Lab Med 109:984–985

  22. Gould VE, Moll R, Moll I, Lee I, Franke WW (1985) Biology of disease. Neuroendocrine (Merkel) cells of the skin: Hyperplasias, dysplasias, and neoplasms. Lab Invest 52:334–353

  23. Gown AM, Vogel AM (1985) Monoclonal antibodies to human intermediate filament proteins. III. Analysis of tumours. Am J Clin Pathol 84:413–424

  24. Hedberg KK, Chen LB (1986) Absence of intermediate filaments in a human adrenal cortex carcinoma-derived cell line. Exp Cell Res 163:509–517

  25. Holthoefer H, Miettinen M, Paasivuo R, Lehto V-P, Linder E, Alfthan O, Virtanen I (1983) Cellular origin and differentiation of renal carcinomas: A fluorescence microscopy study with kidney specific antibodies, anti-intermediate filament antibodies and lectins. Lab Invest 49:317–326

  26. Hsu SM, Raine L, Fanger H (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: A comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29:577–580

  27. Kerl H, Hofler H, Rauch JH, Denk H (1984) New immunochemical observations in cutaneous neuroendocrine carcinoma (Merkel cell tumour) (abstr). J Invest Dermatol 82:541–542

  28. Krepler R, Denk H, Artlieb U, Moll R (1982) Immunocytochemistry of intermediate filament proteins present in pleomorphic adenomas of the human parotid gland: Characterization of different cell type in the same tumour. Differentiation 21:191–199

  29. LaRocca PJ, Rheinwald JG (1984) Coexpression of simple epithelial keratins and vimentin by human mesothelium and mesothelioma in vivo and in culture. Cancer Res 44:2991–2999

  30. Ljungberg O, Ericsson UB, Bondeson L, Thorell J (1983) A compound follicular-parafollicular cell carcinoma of the thyroid: A new tumour entity? Cancer 52:1053–1061

  31. McNutt MA, Bolen JW, Gown AM, Hammar SP, Vogel AM (1985) Coexpression of intermediate filaments in human epithelial neoplasms. Ultrastruct Pathol 9:31–43

  32. Merot Y, Margolis RJ, Dahl D, Saurat J-H, Mihm MC (1986) Coexpression of neurofilament and keratin proteins in cutaneous neuroendocrine carcinoma cells. J Invest Dermatol 86:74–77

  33. Miettinen M, Franssila K, Lehto VP, Passivuo R, Virtanen I (1984) Expression of intermediate filament proteins in thyroid gland and thyroid tumours. Lab Invest 50:262–270

  34. Miettinen M, Lehto VP, Dahl D, Virtanen I (1985) Varying expression of cytokeratin and neurofilaments in neuroendocrine tumours of human gastrointestinal tract. Lab Invest 52:429–436

  35. Moll R, Franke WW, Schiller DL, Geiger B, Krepler R (1982) The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumours and cultured cells. Cell 31:11–24

  36. Moll R, Krepler R, Franke WW (1983) Complex cytokeratin polypeptide pattern observed in certain human carcinomas. Differentiation 23:256–269

  37. Moll R, Moll I, Franke WW (1984) Identification of Merkel cells in human skin by specific cytokeratin antibodies: Changes of cell density and distribution in fetal and adult plantar epidermis. Differentiation 28:136–154

  38. Moll R, Franke WW (1985) Cytoskeletal differences between human neuroendocrine tumours: A cytoskeletal protein of molecular weight 46,000 distinguishes cutaneous from pulmonary neuroendocrine neoplasms. Differentiation 30:165–175

  39. Moll R, Cowin P, Kapprell H-P, Franke WW (1986) Biology of disease. Desmosomal proteins: New markers for identification and classification of tumours. Lab Invest 54:4–25

  40. Osborn M, Weber K (1983) Tumour diagnosis by intermediate filament typing: a novel tool for surgical pathology. Lab Invest 48:372–394

  41. Osborn M, Debus E, Weber K (1984) Monoclonal antibodies specific for vimentin. Eur J Cell Biol 34:137–143

  42. Permanetter W, Nathrath WBJ, Löhrs U (1982) Immunohistochemical analysis of thyroglobulin and keratin in benign and malignant thyroid tumours. Virchows Arch [Pathol Anat] 398:221–228

  43. Schmid E, Osborn M, Rungger-Brändle E, Gabbiani G, Weber K, Franke WW (1980) Distribution of vimentin and desmin filaments in smooth muscle tissue of mammalian and avian aorta. Exp Cell Res 137:329–340

  44. Viac J, Reano A, Brochier J, Staquet MJ, Thivolet J (1983) Reactivity pattern of a monoclonal anti-keratin antibody (KL 1). J Invest Dermatol 81:351–354 (1983)

  45. Venetianer A, Schiller DL, Magin T, Franke WW (1983) Cessation of cytokeratin expression in a rat hepatoma cell line lacking differentiated functions. Nature 305:730–733

  46. Waldherr R, Schwechheimer K (1985) Co-expression of cytokeratin and vimentin intermediate-sized filaments in renal cell carcinoma. Comparative study of the intermediate-sized filamentdistribution in renal cell carcinomas and normal human kidney. Virchows Arch [Pathol Anat] 408:15–27

  47. Wiedenmann B, Franke WW, Kuhn C, Moll R, Gould VE (1986) Synaptophysin: A marker protein for neuroendocrine cells and neoplasms. Proc Natl Acad Sci (USA) 83:3500–3504

  48. Yagi Y, Yagi S, Saku T (1985) The localization of cytoskeletal proteins and thyroglobulin in thyroid microcarcinoma in comparison with clinically manifested thyroid carcinoma. Cancer 56:1967–1971

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Correspondence to Sören Schröder.

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Dedicated to Prof. Dr. G. Seifert on the occasion of his 65th birthday

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Schröder, S., Dockhorn-Dworniczak, B., Kastendieck, H. et al. Intermediate-filament expression in thyroid gland carcinomas. Vichows Archiv A Pathol Anat 409, 751–766 (1986). https://doi.org/10.1007/BF00710761

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Key words

  • Thyroid carcinoma
  • Intermediate filaments
  • Keratin
  • Vimentin
  • Neurofilaments