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Oocytes from pachytene to dictyotene can easily be analysed in neonatal rodents

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We have investigated the dynamics of meiotic prophase I in neonatal ovaries from different wild rodent species, from a laboratory strain ofMus musculus and fromMus musculus × Mus spretus F1 hybrids. We found that almost all stages of prophase I were regularly present in neonatal ovaries from these species and that their transcriptional activity can be assessed by [3H]-uridine incorporation, indicating that postnatal analysis of meiotic chromosomes and synapsis may be conducted as an alternative to the investigation of foetal ovaries.

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  1. Bachvarova R (1985) Gene expression during oogenesis and oocyte development in mammals.Dev Biol 1: 453–524.

  2. Baker T (1972) Oogenesis and ovarian development. In: Balin H, Glasser SR, eds.Reproductive Biology, Excerpta Medica, 398–437.

  3. Bakken AH, McClanahan M (1978) Patterns of RNA synthesis in early meiotic prophase oocytes from fetal mouse ovaries.Chromosoma 67: 21–40.

  4. Dietrich AJJ, Mulder RJP (1983) A light- and electron microscopic analysis of meiotic prophase in female mice.Chromosoma 88: 377–385.

  5. Fletcher JM (1979) Light microscopic analysis of meiotic prophase chromosomes by silver staining.Chromosoma 72: 241–248.

  6. Lemon JG, Morton WRM (1968) Oogenesis in the golden hamster (Mesocricetus auratus). A study of the first meiotic prophase.Cytogenetics 7: 376–389.

  7. Matsuda Y, Chapman VM (1992) Analysis of sex-chromosomes aneuploidy in interspecific backcross progeny between the laboratory mouse strain C57BL 6 andMus spretus.Cytogenet Cell Genet 60: 74–78.

  8. Meredith R (1969) A simple method for preparing meiotic chromosomes from mammalian testis.Chromosoma 26: 254–258.

  9. Moses MJ, Poorman PA (1984) Synapsis, Synaptic adjustment and DNA synthesis in mouse oocytes. In: Bennet MD, Gropp A, Wolf U, eds.Chromsomes Today 8, George Allen and Unwin, pp 90–103.

  10. Simoni G, Branbati B, Danesino C,et al. (1983) Efficient direct chromosome analysis and enzyme determinations from chorionic villi samples in the first trimester of pregnancy.Hum Genet 63: 349–357.

  11. Singh AP, Raman R (1993) Mammalian sex chromosomes. Vl. Synapsis in the heterochromatin-rich X chromosomes of four rodent species,Mus dunni, Bandicota bengalensis, Mesocricetus auratus, andNesokia indica.Genome 36: 195–198.

  12. Speed RM (1982) Meiosis in the foetal mouse ovary. I. An analysis at the light microsope level using surface-spreading.Chromosoma 85: 427–437.

  13. Speed RM (1990) Meiosis in mammals and man.Adv Mutagenesis Res 1: 27–69.

  14. Sperling K, Kalscheuer V, Neitzel H (1987) Transcriptional activity of constitutive heterochromatin in the mammalMicrotus agrestis (Rodentia, Cricetidae),Exp Cell Res 173: 463–472.

  15. Sung WK, Jagiello G (1982) A technique for light and electron microscopy of the synaptonemal complex of the mouse oocyte.Can J Genet Cytol 24: 675–680.

  16. von Winiwarter H (1901) Recherches sur l'ovogenèse et l'organogenèse de l'ovaire des mammifères (lapin et homme).Arch Biol 17: 33–200.

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Singh, A.P., Maerzke, S., von Deimling, O. et al. Oocytes from pachytene to dictyotene can easily be analysed in neonatal rodents. Chromosome Res 1, 209–213 (1993). https://doi.org/10.1007/BF00710126

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Key words

  • meiosis
  • oocytes
  • synaptonemal complex