Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Molecular analysis of chromosomal polymorphism in the South American cricetid,Graomys griseoflavus

  • 19 Accesses

  • 7 Citations

Abstract

Graomys griseoflavus is a South American phyllotine rodent widespread in Argentina that shows a high frequency of Robertsonian fusions (RFs). DNA restriction withEcoRI produced a 250-bp repeated family (EG250) specific for the genus. Southern hybridization and sequencing analysis indicate that the EG250 family is heterogeneous, comprising at least two subfamilies.In situ hybridized EG250 probe showed a centromere location in almost all chromosomes. In all karyomorphs C-banding was negative, but restriction enzyme banding (re-banding) withAluI andMboI showed centromeric blocks in the autosomes that will generate Robertsonian fusions. Thus, we found three groups of chromosomes: (a) EG250 and Re-banding negative; (b) EG250 positive and Re-banding negative; and (c) EG250 and Re-banding positive. We consider that group (b) is more the result of chromatin condensation state than that of the frequency of recognition sites for the enzymes used. Restriction enzyme blocks would appear in regions with heterochromatic EG250 subfamilies, while lack of banding would be due to decondensed EG250 subfamilies becoming an easier target for chromosomal restriction. It is suggested that heterochromatic EG250 DNA provides a favourable molecular environment for Robertsonian fusion occurrence.

This is a preview of subscription content, log in to check access.

References

  1. Arn PH, Jabs EW (1990) Characterization of human centromeric regions using restriction enzyme banding, alphoid DNA and structural alterations.Mol Biol Med 7: 371–377.

  2. Baker RJ, Bickham JW (1986) Speciation by monobrachial centric fusions.Proc Natl Acad Sci USA 83: 8245–8248.

  3. Bianchi NO, Bianchi MS (1987) Analysis of the eukaryotic chromosome organization with restriction endonucleases. In: Obe G, Basler A, eds.Cytogenetics. Berlin: Springer, pp 208–299.

  4. Bianchi MS, Bianchi NO, Pantelias GE, Wolff S (1985) The mechanism and pattern of banding induced by restriction endonucleases in human chromosomes.Chromosoma 91: 131–136.

  5. Bianchi MS, Bianchi NO, Gripenberg U, Wessman M, Huuhtanen S (1990) Characterization of heterochromatin in moose (Alces alces).Genetica 80: 1–7.

  6. Bogenberger JM, Neitzel H, Fittler F (1987) A highly repetitive DNA component common to all Cervidae: its organization and chromosomal distribution during evolution.Chromosoma 95: 154–161.

  7. Broccoli D, Trevor KT, Miller OJ, Miller DA (1991) Isolation of a variant family of mouse minor satellite DNA that hybridizes preferentially to a chromosome 4.Genomics 10: 68–74.

  8. Brutlag DL (1980) Molecular arrangement and evolution of heterochromatic DNA.Annu Rev Genet 14: 121–144.

  9. Burkholder G (1989) Morphological and biochemical effects of the endonucleases on isolated mammalian chromosomesin vitro.Chromosoma 97: 347–355.

  10. Elder FFB, Hsu TC (1988) Tandem fusion in the evolution of mammalian chromosomes. In: Daniel A, ed.The Cytogenetics of Mammalian Autosomal Rearrangements. New York: Liss, pp 481–506.

  11. Garagna S, Redi CA, Capanna Eet al. (1993) Genome distribution, chromosomal allocation, and organization of the major and minor satellite DNAs in 11 species and subspecies of the genusMus.Cytogenet Cell Genet 64: 247–255.

  12. Gosalvez J, Lopez-Frenandez C, Ferrucci L, Mezzanotte R (1989) DNA base sequence is not the only factor for restriction endonuclease activity on metaphase chromosomes: evidence using isosquizomers.Cytogenet Cell Genet 50: 142–144.

  13. Gardner AL, Patton JL (1976) Karyotypic variation in oryzomine rodents (Cricetidae) with comments on chromosomal evolution in the neotropical cricetine complex.Occasional Papers of the Museum of Zoology, No. 49, Louisiana State University, 1–48.

  14. Hamilton MJ, Honeycutt RL, Baker RJ (1990) Intragenomic movement, sequence amplification and concerted evolution in satellite DNA in harvest mice.Reithrodontomys: evidence from in situ hybridization.Chromsoma 99: 321–329.

  15. Hamilton MJ, Hong G, Wichman HA (1992) Intragenomic movement and concerted evolution of satellite DNA inPeromyscus: evidence fromin situ hybridization.Cytogenet Cell Genet 60: 40–44.

  16. Hoerz W, Zachau HG (1977) Characterization of distinct segments in mouse satellite DNA by restriction nucleases.Eur J Biochem 73: 383–392.

  17. John B (1988) The biology of heterochromatin. In Verma RS, ed.Heterochromatin: Molecular and Structural Aspects. Cambridge: Cambridge University Press, pp 1–147.

  18. Mezzanotte R, Rossino R, Nieddu M, Lopez-Frenandez C, Gosalvez J (1992) The DNA fragments produced byAluI andBstNI digestion of fixed mouse chromosomes.Chromosoma 101: 641–644.

  19. Miller DA, Choi YC, Miller DJ (1983) Chromosome localization of highly repetitive human DNAs and amplified ribosomal DNA with restriction enzymes.Science 219: 395–397.

  20. Miller JR, Hindkjær J, Thomsen PD (1993) A chromosomal basis for the differential organization of a porcine centromere-specific repeat.Cytogenet Cell Genet 62: 37–41.

  21. Modi WS (1993a) Heterogeneity in the concerted evolution process of a tandem satellite array in meadow mice (Microtus).J Mol Evol 37: 48–56.

  22. Modi WS (1993b) Comparative analyses of heterochromatin inMicrotus: sequence heterogeneity and localized expansion and contraction of satellite DNA arrays.Cytogenet Cell Genet 62: 142–148.

  23. Pardue ML (1985)In situ hybridization. In: Hames BD, Higgins SJ, eds.Nucleic Acid Hybridization. Oxford: IRL Press, pp 179–202.

  24. Redi CA, Capanna E (1988) Robertsonian heterozygotes in the house mouse and fate of their germ cells. In: Daniel A, ed.The Cytogenetics of Mammalian Autosomal Rearrangements, New York: Liss, pp 315–359.

  25. Redi CA, Garagna S, Mazzini G, Winking H (1986) Pericentromeric heterochromatin and A-T contents during Robertsonian fusion in the house mouse.Chromosoma 94: 31–35.

  26. Redi CA, Garagna S, Capanna E (1990) Nature's experiment within situ hybridization? A hypothesis for the mechanism of Rb fusion.J Evol Biol 3: 133–137.

  27. Rose RE (1988). The nucleotide sequence of pACYC184.Nucleic Acids Res 16: 335.

  28. Sambrook J, Fritsch EF, Maniatis R (1989)Molecular Cloning: A Laboratory Manual. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.

  29. Sanger F, Niclen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors.Proc Natl Acad Sci USA 74: 5463–5467.

  30. Schweizer D, Loidl J, Hamilton B (1987) Heterochromatin and the phenomenon of chromosome banding. In: Henning W ed.Structure and Function of Eukaryotic Chromsomes. Results and Problems in Cell Differentiation, No 14. Berlin: Springer, pp 235–254.

  31. Sumner AT (1972) A simple technique for demonstrating centromeric heterochromatin.Exp Cell Res 75: 304–306.

  32. Van Den Bussche RA, Baker RJ, Wichman HA, Hamilton MJ (1993) Molecular phylogenetics of Stenodermatini bat genera: congruence of data from nuclear and mitochondrial DNA.Mol Biol Evol 10: 944–959.

  33. Vidal-Rioja L, de Fronza TG, Wainberg R, Brum-Zorrilla N, Wallace F, Zambelli A (1988) C-banding pattern and satellite DNA localization on the chromosomes ofOryzomys flavescens (Rodentia, Cricetidae).Caryologia 41: 323–328.

  34. Waye JS, Willard HF (1991) Nucleotide sequence heterogeneity of alpha satellite repetitive DNA: a survey of alphoid sequences from different human chromosomes.Nucleic Acids Res 15: 7549–7569.

  35. Wichman HA, Payne CT, Ryder OA, Hamilton MJ, Maltbie M, Baker RJ (1991) Genomic distribution of heterochromatic sequences in equids: implications to rapid chromosomal evolution.J Hered 82: 369–377.

  36. Zambelli A, Vidal-Rioja L, Wainberg R (1994) Cytogenetic analysis of autosomal polymorphism inGraomys griseoflavus (Rodentia, Cricetidae).Z. Säugetierkd 59: 14–20.

Download references

Author information

Correspondence to Lidia Vidal-Rioja.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Zambelli, A., Vidal-Rioja, L. Molecular analysis of chromosomal polymorphism in the South American cricetid,Graomys griseoflavus . Chromosome Res 3, 361–367 (1995). https://doi.org/10.1007/BF00710017

Download citation

Key words

  • chromosomal polymorphism
  • heterochromatin
  • satellite DNA
  • South American rodent