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Grouping of soil bacteria by analysis of colony formation on agar plates

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The colony formation of soil bacteria was studied in relation to incubation time. The process was simulated by a colony-forming curve (CFC) which was a superposition of several component curves (cCFC) given theoretically by the first-order reaction (FOR) model. This model had been previously proposed to define the colony formation of cells of a single culture. Soil bacteria were divided into several groups by these cCFC, as different types of bacteria produced their own colonies. Bacteria belonging to a single group grew at a similar rate on the plating medium and each group was characterized by a different growth rate. Most copiotrophic bacteria were fast growers and most oligotrophic bacteria slow growers.

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Correspondence to T. Hattori.

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Hashimoto, T., Hattori, T. Grouping of soil bacteria by analysis of colony formation on agar plates. Biol Fert Soils 7, 198–201 (1989). https://doi.org/10.1007/BF00709648

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Key words

  • Plate technique
  • Colony formation
  • Growth rate
  • Bacterial grouping
  • Oligotrophic bacteria
  • Copiotrophic bacteria