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Ex vivo determination of potentially virulent Sporothrix schenckii

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Abstract

Hyphae from 30 isolants ofSporothrix andOphiostoma species were washed, dried and pyrolyzed at 350°C. Pyrolysis products were separated on a Carbowax column heated 7.5°C/min to and maintained for 50 min at 160°C. Hydrogen flame detector responses were recorded graphically. Fifteen clinical isolants ofS. schenckii from geographically separated sources produced qualitatively identical pyrograms.S. foliorum, 8 avirulentS. schenckii and otherSporothrix species isolants from soils, andSporothrix states of 6Ophiostoma species yielded pyrograms readily distinguished from each other and from those of virulentS. schenckii. Taxonomic and clinical implications of the pyrograms are mentioned.

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References

  1. 1.

    Hoog, G.S. de. 1974. The genera Blastobotrys, Sporothrix, Calcarisporium and Calcarisporiella gen. nov. Stud. Mycol. (Baarn) 7: 1–84.

  2. 2.

    Weijman, A.C.M. & G.S. de Hoog. 1975. On the subdivision of the genus Ceratocystis. Ant. van Leeuwen. 41: 353–360.

  3. 3.

    Howard, D.H. & G.F. Orr. 1963. Comparison of strains of Sporotrichum schenckii isolated from nature. J. Bact. 85: 816–821.

  4. 4.

    Ishizaki, H., R. Wheat & N.F. Conant. 1974. Serological cross reactivity between Sporothrix schenckii and Ceratocystis species. Abst. Ann. Mtg. Amer. Soc. Microbiol., p. 140.

  5. 5.

    Kulik, M. M. & P. G. Vincent 1973. Pyrolysis — gas liquid chromatography of fungi. Mycopathol Mycol. Appl. 51: 1–18; 251–265.

  6. 6.

    Mackinnon, J.E., I.A. Conti-Diaz, E. Gezuele, E. Civila & S. da Luz. 1969. Isolation of Sporothrix schenckii from nature and considerations of its pathogenicity and ecology. Sabouraudia 7: 38–45.

  7. 7.

    Nicot, J. & F. Mariat. 1973. Caracteres morphologiques et position systematique de Sporothrix schenckii, agent de la sporotrichose humaine. Mycopathol. Mycol. Appl. 49: 53–65.

  8. 8.

    Nishikawa, T., T. Harada, S. Harada & H. Hatano. 1975. Serologic differences in strains of Sporothrix schenckii. Sabouraudia 13: 285–290.

  9. 9.

    Rush-Munro, F.M. 1971. Personal communication.

  10. 10.

    Sprung, D. G. & D. E. Wujek. 1971. Chemotaxonomic studies of Pleurastrum Chodat by means of pyrolysis — gas — liquid chromatography. Phycol. 10: 251–254.

  11. 11.

    Taylor, J.J. 1970. A comparison of some Ceratocystis species with Sporothrix schenckii. Mycopathol. Mycol. Appl. 42: 233–240.

  12. 12.

    Travassos, L.R., P.A.J. Gorin & K.O. Lloyd. 1973. Comparison of the rhamnomannans from the human pathogen Sporothrix schenckii with those from the Ceratocystis species. Inf. Immun. 8: 686–693.

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Taylor, J.J. Ex vivo determination of potentially virulent Sporothrix schenckii. Mycopathologia 58, 107–114 (1976). https://doi.org/10.1007/BF00707181

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Keywords

  • Pyrolysis — gas — liquid chromatography
  • Sporothrix schenckii
  • Ophiostoma (Ascomyceteae)
  • clinical mycology