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The molecular analysis ofbrown eye color mutations isolated from geographically discrete populations ofDrosophila melanogaster

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Abstract

A large proportion of spontaneous mutations inDrosophila melanogaster strains of laboratory origin are associated with insertions of mobile DNA elements. As a first step toward determining whether spontaneous laboratory mutations are predictive for mutational events occurring in the wild, recessivebrown (bw) eye color mutants were isolated. By inbreeding the progeny of wild-caughtDrosophila melanogaster females,bw mutations were isolated from seven separate geographic sites distributed among Japan, California, Siberia and Hungary. Among a total of 14 mutations studied, no case of transposon mutagenesis was found. At least 4 mutations are associated with small deletions in thebw gene. The remainder are inseparable from wild-typebw by Southern analysis and are presumed to be basepair changes or very small indels. Although only two spontaneousbw mutants of laboratory origin have been analyzed molecularly, one is a mobile element insertion.

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Correspondence to Eiji Nitasaka.

Additional information

Communicated by M. Sekeguchi

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Nitasaka, E., Yamazaki, T. & Green, M.M. The molecular analysis ofbrown eye color mutations isolated from geographically discrete populations ofDrosophila melanogaster . Molec. Gen. Genet. 247, 164–168 (1995). https://doi.org/10.1007/BF00705646

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Key words

  • Spontaneous mutations
  • Natural populations
  • Drosophila melanogaster