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Application of protein-A indirect ELISA (PAI-ELISA) for the detection of anti-Smith antibodies in systemic lupus erythematosus patients

Abstract

An indirect form of protein-A ELISA (PAI-ELISA) was optimized and, when used to detect anti-Smith antibodies in sera of 31 systemic lupus erythematosus (SLE) patients, gave results comparable with those using a commercial immunodiffusion kit. The number of sera found to be positive for anti-Smith antibodies by ELISA was seven, four of which were also found positive by immunodiffusion.

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References

  1. AhmadI.B., AnuarN., SalamF., AhmadN. & AliA.M. 1989 Serology in the detection, assay and diagnosis of antigen and antibodies. InCurrent Research in Life Sciences, eds ZakriA.H. & LatifA. pp. 171–183. Kuala Lumpur: Faculty of Life Sciences, Universiti Kebangsaan Malaysia. (In Malay.)

  2. BanttarriE.E. & PetersenA.C. 1983 A cleaning technique for cuvettes used in enzyme-linked immunosorbent assay.Plant Disease 67, 18–20.

  3. BaradaF.A., AndrewsB.S., DavisJ.S. & TaylorR.P. 1981 Antibodies to Sm in patients with systemic lupus erythematosus.Arthritis and Rheumatism 24, 1236–1244.

  4. ClarkM.F. & AdamA.N. 1977 Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses.Journal of General Virology 34, 475–483.

  5. CroweW. & KushnerI. 1977 An immunofluorescent method usingCrithidia luciliae to detect antibodies to double-stranded DNA.Arthritis and Rheumatism 20, 811–814.

  6. EdwardsM.L. & CooperJ.I. 1985 Plant virus detection using a new form of indirect ELISA.Journal of Virological Methods 11, 309–319.

  7. EngvallE. & PearlmannP. 1971 Enzyme-linked immunosorbent assay (ELISA): quantitative assay of immunoglobulin G.Immunochemistry 8, 871–874.

  8. FritzlerM. J. 1986 Immunofluorescent antinuclear antibody tests. InManual of Clinical Laboratory Immunology, 3rd edn, eds RoseN.R. FriedmanH. & FaheyJ.L. pp. 733–749. Washington DC: American Society for Microbiology.

  9. GoodwinP.H. & BanttariJ.E. 1984 Increased sensitivity of ELISA for potato viruses S, X, and Y by polystyrene pretreatments, additives, and a modified assay procedure.Plant Disease 68, 944–947.

  10. LernerM.R. & SteiztJ.A. 1979 Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus.Proceedings of the National Academy of Sciences of the United States of America 76, 5495–5499.

  11. NakamuraR.M., PeeblesC.L., MoldenD.P. & TanE.M. 1984 Advances in laboratory test for autoantibodies to nuclear antigens in systemic rheumatic disease.Laboratory Medicine 15, 190–198.

  12. NotmanD.D., KurataN. & TanE.M. 1975 Profiles of antinuclear antibodies in systemic rheumatic diseases.Annals of Internal Medicine 83, 464–469.

  13. ReichlinM. 1976 Problems in differentiating SLE and mixed connective-tissue disease.New England Journal of Medicine 295, 1194–1196.

  14. SharpG., IrwinW., MayC.M. HolmanH.R., McDuffieF.C., HessE.V. & SchmidF.R. 1976 Association of antibodies to ribonucleoprotein and Sm antigens with mixed connective tissue disease, systemic lupus erythematosus and other rheumatic diseases.New England Journal of Medicine 295, 1149–1154.

  15. Siti-RohanaB.O., AhmadI.B. & NasurruddinB.A. 1992 Prevalence of antinuclear antibodies in Malaysian subjects. In:Medical Research Towards the 21st Century, eds AlauddenS., MoosdeenF., RajikinM.H., SaimL. & DaudM.Z. pp. 324–327. Kuala Lumpur: Medical Faculty, Universiti Kebangsaan Malaysia.

  16. SutulaC.L., GillettJ.M., MorisseyS.M. & RamsdellD.C. 1986 Interpreting ELISA data and establishing the positive-negative threshold.Plant Disease 70, 722–727.

  17. TakanoM., GoldenS.S., SharpG.C. & AgrisP.F. 1981 Molecular relationship between two nuclear antigens ribonucleoproteins and Sm: purification of active antigens and their biochemical characterisation.Biochemistry 20, 5929–5935.

  18. TanE.M. 1982 Autoantibodies to nuclear antigens (ANA); their immunobiology and medicine. In:Advances in Immunology Vol 33, eds KunkelH.G. & DixonF.J. pp. 167–240. New York: Academic Press.

  19. TanE.M. 1983 Antinuclear antibodies in diagnosis and management. InThe Biology of Immunologic Disease, eds DixonF.J. & FisherD.W., pp. 319–324. Sunderland, MA: Sinauer Associates.

  20. TanE.M., CohenA.S., FriesJ.F., MasiA.T., McShaneA.J., RothfieldN.F., SchallerJ.G., TalalN. & WinchesterJ.G. 1982 The 1982 revised criteria for the classification of systemic lupus erythematosus.Arthritis and Rheumatism 25, 1271–1277.

  21. WinnD.M., WolfeJ.F., LinbergD.A., FristoeF.H., KinglandL. & SharpG.C. 1979 Identification of a clinical subset of systemic lupus erythematosus by antibodies to the Sm antigen.Arthritis and Rheumatism 22, 1334–1337.

  22. Zainal-AbidinB.A.H., IdrisS.R.H., DolahS. & AhmadI.B. 1992 PAI-ELISA technique for the detection of antibodies against rodent malaria.Sains Malaysiana 21, 133–142. (In Malay.)

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Additional information

B.O. Siti-Rohana is with the Universiti Kebangsaan Malaysia (UKM), Faculty of Medicine, Kuala Lumpur, Malaysia. I.B. Ahmad is with the Department of Microbiology, Faculty of Life Sciences, Universiti Kebangsaan Malaysia, Bangi, 43600 UKM, Malaysia; B.A. Nasaruddin is with the Institute for Medical Research, Malaysia.

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Siti-Rohana, B.O., Ahmad, I.B. & Nasuruddin, B.A. Application of protein-A indirect ELISA (PAI-ELISA) for the detection of anti-Smith antibodies in systemic lupus erythematosus patients. World J Microbiol Biotechnol 11, 206–208 (1995). https://doi.org/10.1007/BF00704650

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Key words

  • Anti-Smith antibodies
  • ELISA
  • protein A
  • systemic lupus erythematosus