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Protein production from whey usingPenicillium cyclopium; growth parameters and cellular composition

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The growth parameters ofPenicillium cyclopium have been evaluated in a continuous culture system for the production of fungal protein from whey. Dilution rates varied from 0.05 to 0.20 h−1 under constant conditions of temperature (28°C) and pH (3.5). The saturation coefficients in the Monod equation were 0.74 g l−1 for lactose and 0.14 mg l−1 for oxygen, respectively. For a wide range of dilution rates, the yield was 0.68 g g−1 biomass per lactose and the maintenance coefficient 0.005 g g−1 h−1 lactose per biomass, respectively. The maximum biomass productivity achieved was 2 g l−1 h−1 biomass at dilution rates of 0.16–0.17 h−1 with a lactose concentration of 20 g l−1 in the feed. The crude protein and total nucleic acid contents increased with a dilution rate, crude protein content varied from 43% to 54% and total nucleic acids from 6 to 9% in the range of dilution rates from 0.05 to 0.2 h−1, while the Lowry protein content was almost constant at approximately 37.5% of dry matter.

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(mg l−1):

Co initial concentration of dissolved oxygen


D dilution rate

(mg l−1):

K02 saturation coefficient for oxygen

(g l−1):

Ks saturation coefficient for substrate

(g g−1 h−1) lactose per biomass):

m maintenance energy coefficient

(mM g−1 h−1O2 per biomass):

Q02 specific oxygen uptake rate

(g l−1):

S residual substrate concentration at steady state

(g l−1):

So initial substrate concentration in feed


t1/2 time when Co is equal to Co/2

(g l−1):

X biomass concentration

(g l−1):

X biomass concentration at steady state

(g g−1 biomass per lactose):

YG yield coefficient for cell growth

(g g−1 biomass per lactose):

Yx/s overall yield coefficient


μ specific growth rate


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Correspondence to J. H. Kim.

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Kim, J.H., Lebeault, J.M. Protein production from whey usingPenicillium cyclopium; growth parameters and cellular composition. European J. Appl. Microbiol. Biotechnol. 13, 151–154 (1981). https://doi.org/10.1007/BF00703044

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  • Biomass
  • Lactose
  • Crude Protein
  • Growth Parameter
  • Dilution Rate