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Untersuchungen zur Entwicklung des PhycomycetenAllomyces arbuscula

II. Einfluß von Inhibitoren der Protein- und Nucleinsäuresynthese auf die Gametogenese

Studies on development of the phycomyceteAllomyces arbuscula

II. Effect of inhibitors of protein and nucleic acid synthesis on gametogenesis

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Abstract

InAllomyces arbuscula formation of gametes occurs within 80 min in isolated gametangia. Gametogenesis shows to be sensitive to cycloheximide at 50 and 100 μg/ml, while actinomycin D at 25 and 50 μg/ml fails to inhibit gametogenesis. Synthesis ofrRNA can be profoundly inhibited by 3×10-3 M 5-fluorouracil prior to gametogenesis, without any effect on differentiation of gametes. It is shown by polyacrylamide-gel electrophoresis that during gametogenesis radioactive phosphate is incorporated intotRNA, but not intorRNA. The results indicate that formation of gametes is dependent uponmRNA already present in the gametangia before induction of gametogenesis. It is concluded further that protein synthesis on cytoplasmic ribosomes is finished 20–30 min after induction and thatrRNA synthesis seems not to be a prerequisite for the differentiation of gametes.

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Literatur

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  2. Fähnrich, P.: Untersuchungen zur Entwicklung des PhycomycetenAllomyces arbuscula. I. Einfluß von Inhibitoren der Protein- und Nucleinsäuresynthese auf die Differenzierung von Gametangien. Arch. Microbiol.98, 85–92 (1974)

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Fähnrich, P. Untersuchungen zur Entwicklung des PhycomycetenAllomyces arbuscula . Arch. Microbiol. 99, 147–153 (1974). https://doi.org/10.1007/BF00696230

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Key words

  • Allomyces arbuscula
  • Actinomycin D
  • Cycloheximide
  • 5-Fluorouracil
  • Gametogenesis
  • RNA Synthesis