Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Inactivation of neurohormone D by Malpighian tubules in an insect,Periplaneta americana

Summary

The heart rate accelerating peptide neurohormone D is rapidly inactivated by intact Malpighian tubules of cockroaches and also by homogenates of them. The peptide is removed from a solution by an active uptake mechanism. Within the tubule cells one or a set of soluble proteinases with a molecular mass around 45000 Da hydrolyze the neuropeptide. The inhibition of the reaction by synthetic protease inhibitors and chelating agents characterizes the enzyme(s) as metalloendopeptidase with serine or cysteine at the active site. This seems to be the first evidence that a peptidase comparable to the neutral metalloendopeptidase of mammalian kidney microvilli exists in insect Malpighian tubules and could play an important role in the hydrolysis of neuropeptides.

This is a preview of subscription content, log in to check access.

Abbreviations

EDTA :

ethylenediamine tetraacetic acid

AEBSF :

4-β-aminoethylbenzenesulfonylfluoride

PMSF :

pnenylmethanesulfonyl fluoride

TLCK :

tosyl-lysine chloromethyl ketone

TPCK :

tosyl-phenylalanyl chloromethyl ketone

CMB :

chloromercuribenzoic acid

DNP-Ala :

N-dinitrophenyl-alanine

TFA :

trifluoroacetic acid

References

  1. Baumann E, Gersch M (1982) Purification and identification of neurohormone D, a heart accelerating peptide from the corpora cardiaca of the cockroachPeriplaneta americana. Insect Biochem. 12:7–14

  2. Baumann E, Penzlin H (1984) Sequence analysis of neurohormone D, a neuropeptide of an insect,Periplaneta americana. Biomed Biochim Acta 43:K13–16

  3. Baumann E, Gersch M, Penzlin H (1984) The occurrence of neurohormone D in the nervous system of several arthropods. Zool Jb Physiol 88:213–223

  4. Birkenbeil H (1971) Untersuchungen zur Wirkung von Neurohormon D und von anderen aktiven Substanzen auf das Zentralnervensystem der SchabePeriplaneta americana (L.). Zool Jb Physiol 75, 493–525

  5. Bond JS, Beynon RJ (1985) Mammalian metalloendopeptidases. Int J Biochem. 17:565–574

  6. Bruton CJ, Hartley BS (1970) Chemical studies on methionyl-t-RNA synthetase fromEscherichia coli. J Mol Biol 52:165–178

  7. Dua AK, Pinski C, Labella F (1985) Peptidases that terminate the action of enkephalins. Consideration of physiological importance for amino-, carboxy-, endo-, and pseudoenkephalinase. Life Sci 37:985–992

  8. Gäde G (1985) Hypertrehalosaemic hormones and myoactive factors from cockroach corpus cardiacum are very likely identical. Naturwissenschaften 72:95–96

  9. Gersch M, Baumann, E, Birkenbeil H, Pass G, Penzlin H (1982) Die Wirkungsweise des herzaktiven Peptids Neurohormon D vonPeriplaneta americana. Zool Jb Physiol 86:17–33

  10. Gersch M, Richter K (1963) Auslösung von Nervenimpulsen durch ein Neurohormon beiPeriplaneta americana. Zool Jb Physiol 70:301–308

  11. Gold AM, Fahrney D (1964) Sulfonyl fluorides as inhibitors of esterases. II. Formation and reactions of phenylmethane sulfonyl α-chymotrypsin. Biochemistry 3:783–791

  12. Hertel W (1975) Untersuchungen zur Beeinflussung des Herzschlages vonPeriplaneta americana L. unter Bedingungen der Kurzzeitkultur von Herzsegmentpräparaten in vitro. Zool Jb Physiol 79:70–76

  13. Meredith J, Moore L, Scudder GGE (1984) Excretion of ouabain by Malpighian tubules ofOncopeltus fasciatus. Am J Physiol 246:R705-R715

  14. Mordue W, Stone JV (1978) Structure and metabolism of adipokinetic hormone. Abstracts VIIIth ISCE, Amsterdam, p 164

  15. O'Shea M, Witten J, Schaffer M (1984) Isolation and characterization of two myoactive neuropeptides: Further evidence of an invertebrate peptide family. J Neurosci 4:521–529

  16. Phillips E (1982) Hormonal control of renal functions in insects. Fed Proc 41:2348–2354

  17. Richter K, Gersch M (1974) Untersuchungen über die Wirkung cholinerger, aminerger und peptiderger Substanzen am lateralen Herznerven und den funktionellen Zusammenhang dieser Wirkungen bei der Herzregulation vonBlaberus craniifer Burm (Insecta: Blattaria). Zool Jb Physiol 78:16–32

  18. Scarborough RM, Jamieson GC, Kalish F, Kramer StJ, McEnroe GA, Miller CA, Schooley DA (1984) Isolation and primary structure of two peptides with cardio-acceleratory and hyperglycemic activity from the corpora cardiaca ofPeriplaneta americana. Proc Natl Acad Sci 81:5575–5579

  19. Schlottke E (1937) Untersuchungen über die Verdauungsfermente der Insekten. III. Die Abhängigkeit des Fermentgehaltes von der Art der Nahrung. Versuche anPeriplaneta orientalis L. Z Vergl Physiol 24:463–492

  20. Shaw E (1967) Site specific reagent for chymotrypsin and trypsin. Methods Enzymol 11:677–686

  21. Starratt AN, Steele RW (1984) In vivo inactivation of the insect neuropeptide proctolin inPeriplaneta americana Insect Biochem 14:97–102

  22. Steele RW, Starratt AN (1985) In vitro inactivation of the insect neuropeptide proctolin in haemolymph fromPeriplaneta americana. Insect Biochem 15:511–519

  23. Turner A, Matsas R, Kenny J (1985) Are there neuropeptide specific peptidases? Biochem Pharmacol 34:1347–1356

  24. Unger H (1956) Neurohormonale Steuerung der Herztätigkeit bei Insekten. Naturwissenschaften 43:66–67

  25. Walsmann P, Richter M, Markwardt F (1972) Inaktivierung von Trypsin und Thrombin durch 4-Amidinobenzolsulfofluorid und 4-(2-Aminoäthyl)-benzolsulfofluorid. Acta Biol Med Ger 28:577–585

  26. Witten JL, Schaffer MH, O'Shea M, Cook JC, Hemling ME, Rinehart KL (1984) Structures of two cockroach neuropeptides assigned by fast atom bombardment mass spectrometry. Biochem Biophys Res Commun 124:344–349

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Baumann, E., Penzlin, H. Inactivation of neurohormone D by Malpighian tubules in an insect,Periplaneta americana . J Comp Physiol B 157, 511–517 (1987). https://doi.org/10.1007/BF00691836

Download citation

Keywords

  • Peptide
  • Heart Rate
  • Cysteine
  • Serine
  • Molecular Mass