Gnotobiotic puppies were intraperitoneally infected with R252-canine distemper virus (CDV), and treated with normal serum (NS) or CDV-specific antibody. Resultant central nervous system (CNS) lesions were evaluated by light and immunofluorescence microscopy. Lesions in NS-treated dogs, if present, consisted of small foci of neuronal degeneration and accompanying microgliosis, restricted to grey matter of the frontal lobe. Dogs receiving anti-CDV antibody, in contrast, had extensive lesions in the brain. Laminar cortical necrosis, gliosis and astrocytic activation of the grey matter of the frontal lobe was the most consistent lesion observed. In addition, foci of cerebellar granule cell layer degeneration and necrosis was seen along with segmental poliomalacia of the spinal cord. In several dogs, eosinophilic intracytoplasmic and intranuclear viral inclusion bodies were observed in neurons. The lesions observed in these dogs are compared to previously reported lesions of CDV infection in dogs.
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Appel, M. J. G., Gillespie, J. J.: Canine distemper virus. Virol. Monogr.11, 1–96 (1972)
Confer, A. W., Kahn, D. E., Koestner, A., Krakowka, S.: Biological properties of a canine distemper virus isolate associated with demyelinating encephalitis. Infect. Immun.11, 835–844 (1975)
Connolly, J. H., Allen, I. V., Harwitz, L. J., Millar, J. H. D.: Subacute sclerosing panencephalitis. Clinical, pathological, epidemiological and virological findings. Q. J. Med.148, 625–644 (1968)
Hartley, W. J.: A post-vaccinal inclusion body encephalitis in dogs. Vet. Pathol.11, 301–312 (1974)
Innes, J. R. M., Saunders, L. Z.: Canine distemper in comparative neuropathology, pp. 375–384. London: Academic Press 1962
Gibson, J. P., Griesemer, R. A., Koestner, A.: Experimental distemper in the gnotobiotic dog. Vet. Pathol.2, 1–19 (1965)
Griesemer, R. G., Gibson, J. P.: The gnotobiotic dog. Lab An. Care13, 643–649 (1963)
Koestner, A.: Animal model: Distemper-associated demyelinating encephalomyelitis. Am. J. Pathol.78, 361–364 (1975)
Krakowka, S., McCullough, B., Koestner, A., Olsen, R. G.: Myelinspecific autoantibodies associated with central nervous system demyelination in canine distemper virus infection. Infect. Immun.8, 819–827 (1973)
Krakowka, S., Cockerell, G. L., Koestner, A.: Effects of canine distemper virus on lymphoid function in vitro and in vivo. Infect. Immun.11, 1069–1078 (1975)
Krakowka, S., Olsen, R. G., Confer, A. W., Koestner, A., McCullough, B.: Serologic response to canine distemper viral antigens in gnotobiotic dogs infected with R252-canine distemper virus. J. Infect. Dis.132, 384–392 (1975)
Krakowka, S., Koestner, A.: Age-related susceptibility to canine distemper virus infection in gnotobiotic dogs. J. Infect. Dis.134, 629–632 (1976)
Krakowka, S., Koestner, A.: Comparison of canine distemper virus strains: Effects on lymphoid tissues. Am. J. Vet. Res.38, 1919–1922 (1977)
Krakowka, S., Long, D., Mezza, R., Mador, R., Koestner, A.: Derivation and maintenance of gnotobiotic dogs. Lab. Anim. Sci.28, 327–330 (1978)
Krakowka, S., Wallace, A. L., Koestner, A.: Syncitia inhibition by immune lymphocytes: An in vitro test for immunity to canine distemper. J. Clin. Microbiol.7, 292–297 (1978)
Lincohn, S. D., Gorham, J. R., Att, R. L., Hegreberg, G. A.: Etiologic studies of old dog encephalitis I. Demonstration of canine distemper viral antigen in the brain in two cases. Vet. Pathol.8, 1–8 (1971)
Mador, R., Krakowka, S., Koestner, A.: A technique for processing canine brain for combined immunofluorescence, light and electron microscopy. (In preparation)
McCullough, B., Krakowka, S., Koestner, A., Shadduck, J.: Demyelinating activity of distemper virus isolates in gnotobiotic dogs. J. Infect. Dis.130, 343–350 (1974)
McCullough, B., Krakowka, S., Koestner, A.: Experimental canine distemper virus-induced demyelination. Lab. Invest.31, 216–222 (1974)
McCullough, B., Krakowka, S., Koestner, A.: Experimental canine distemper virus-induced lymphoid depletion. Am. J. Pathol.74, 155–176 (1974)
Raine, C. S.: On the development of CNS lesions in natural canine distemper encephalomyelitis. J. Neurol. Sci.30, 13–28 (1976)
Rustigan, R.: Persistent infection of cells in culture by measles virus. II. Effects of measles antibody on persistently infected Hela sublines and recovery of a Hela clonal line persistently infected with incomplete virus. J. Bacteriol.92, 1805–1811 (1966)
Wisniewski, H. M., Raine, C. S., Kay, W. J.: Observations on viral demyelinating encephalomyelitis: Canine distemper. Lab. Invest.26, 589–599 (1974)
Supported by Grant No. ROI A1-09022-07, Allergy and Infectious Diseases, NIH, PHS
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Krakowka, S., Mador, R.A. & Koestner, A. Canine distemper virus-associated encephalitis: Modification by passive antibody administration. Acta Neuropathol 43, 235–241 (1978). https://doi.org/10.1007/BF00691584
- Canine distemper virus
- Passive antibody administration