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Intestinal absorption of amino acids by rainbow trout,Salmo gairdneri (Richardson)

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A technique for the in vitro maintenance of isolated portions of rainbow trout intestine is described. Uptake of14C-L-leucine occurs by an active mechanism which is stereospecific, sodium-dependent and susceptible to inhibition by other neutral amino acids.K t for leucine uptake is 2.72 mM with aV max of 19.61 μmoles/g ethanol extracted dry wt.·10 min. L-valine and L-methionine are competitive inhibitors of L-leucine uptake withK i values of 24.30 mM and 2.56 mM, respectively. Evidence suggests that at least two uptake sites for the transport of neutral amino acids are present in the intestine of this species.

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Ingham, L., Arme, C. Intestinal absorption of amino acids by rainbow trout,Salmo gairdneri (Richardson). J Comp Physiol B 117, 323–334 (1977).

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  • Human Physiology
  • Leucine
  • Rainbow Trout
  • Active Mechanism
  • Competitive Inhibitor