The transport of calcium from mineralized granules stored in the Malpighian tubules to the puparium of the face fly,Musca autumnalis De Geer, was studied. Calcium was transported directly from the tubules to the cuticle via the hemolymph. Little, if any, calcium entered the hindgut or other tissues during or prior to transport. A total of approximately 0.8 mg of calcium per larva was transported, beginning at the wandering stage; peak hemolymph concentrations occurred at anterior retraction. Hemolymph calcium levels subsequently decreased as puparial calcium increased. Puparial mineralization utilized most of the minerals stored during the larval stage, with lesser amounts of minerals being recovered in the adult or excreted. Deposition of mineral salts in the cuticle was accompanied by an increase in cuticular pH from 7.0 to 8.4. The house fly,Musca domestica L., which contains much lower concentrations of minerals in the puparial cuticle, exhibited no increase in cuticular pH during pupariation. Biomineralization of the face fly puparial cuticle appears to occur, in part, as a result of ionic equilibria involving calcium and magnesium phosphates and carbonates, which have relatively low solubility products at alkaline pH.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Cameron JN, Wood CM (1985) Apparent H+ excretion and CO2 dynamics accompanying carapace mineralization in the blue crab (Callinectes sapidus) following moulting. J Exp Biol 114:181–196
Darlington MV, Meyer HJ, Graf G, Freeman TP (1983) The calcified puparium of the face fly,Musca autumnalis (Diptera: Muscidae). J Insect Physiol 29:157–162
Darlington MV, Meyer HJ, Graf G (1985) Carbonic anhydrase in the face fly,Musca autumnalis (DeGeer) (Diptera: Muscidae). Insect Biochem 15:411–418
Eastham L (1925) Peristalsis in the Malpighian tubules of Diptera. Preliminary account: with a note on the elimination of calcium carbonate from the Malpighian tubules ofDrosophila funebris. Q J Microsc Sci 69:385–398
Digby PS (1985) Reducing activity and the formation of base in the shore crab,Carcinus maenas. J Mar Biol Assoc UK 65:505–530
Florkin M, Jeuniaux C (1974) Hemolymph composition. In: Rockstein M. (ed) The physiology of insecta. Academic Press, New York 3: 109–152
Fraenkel G, Hsiao C (1967) Calcification, tanning, and the role of ecdysone in the formation of the puparium of the face fly,Musca autumnalis. J Insect Physiol 13:1387–1394
Gilby AR, McKellar JW (1976) The calcified puparium of a fly. J Insect Physiol 22:1465–1468
Grodowitz MJ, Broce AB (1983) Calcium storage in face fly (Diptera: Muscidae) larvae for puparium formation. Ann Entomol Soc Am 76:418–424
Grodowitz MJ, Broce AB, Kramer KJ (1987) Morphological and biochemical composition of mineralized granules from the Malpighian tubules ofMusca autumnalis DeGeer larvae (Diptera: Muscidae). Insect Biochem 17:335–345
Keilin D (1921) On the calcium carbonate and the calcospherites in the Malpighian tubules and fat body of dipterous larvae and the ecdysial elimination of these products of excretion. Q J Microsc Sci 65:611–625
Krueger RA, Broce AB, Hopkins TL (1987) Dissolution of mineralized granules in the Malpighian tubules of the face fly,Musca autumnalis DeGeer, for use in puparium mineralization. J Insect Physiol 33:255–263
Roseland CR, Grodowitz MJ, Kramer KJ, Hopkins TL, Broce AB (1985) Stabilization of mineralized and sclerotized puparial cuticle of Muscid flies. Insect Biochem 15:521–528
Wood CM, Cameron JN (1985) Temperature and the physiology of intracellular and extracellular acid-base regulation in the blue crab,Callinectes sapidus. J Exp Biol 114:151–179
Contribution No. 87-237-J from the Kansas Agricultural Experiment Station, Kansas State University, Manhattan, Kansas. Cooperative investigation between Agricultural Research Service, U.S. Department of Agriculture and the Kansas Agricultural Experiment Station. K.J.K. is a research chemist and adjunct professor at U.S. Grain Marketing Research Laboratory and Kansas State University, respectively. Mention of a proprietary product in this paper does not imply its approval by the USDA to the exclusion of other products that may also be suitable. Reprint requests to A.B. Broce
About this article
Cite this article
Krueger, R.A., Broce, A.B., Hopkins, T.L. et al. Calcium transport from Malpighian tubules to puparial cuticle ofMusca autumnalis . J Comp Physiol B 158, 413–419 (1988). https://doi.org/10.1007/BF00691138
- Calcium Level
- Larval Stage
- Mineral Salt