Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Different temperature dependences of oxidative phosphorylation in the inner and outer layers of tuna heart ventricle

  • 31 Accesses

  • 18 Citations


  1. 1.

    The oxidative phosphorylation and the temperature dependence of mitochondria prepared from the outer compact layer and the inner spongy layer of adult tuna heart ventricle have been examined.

  2. 2.

    Mitochondria of the inner layer show higher succinoxidase and NADH-oxidase activities as compared with those of the outer layer.

  3. 3.

    Arrhenius plots for succinate oxidation by phosphorylating mitochondria show that the temperature dependence of the inner layer is higher than that of the outer layer.

  4. 4.

    Experiments performed with disrupted non-phosphorylating mitochondria demonstrate that this difference in temperature dependence of the two cardiac compartments depends on the integrity of the mitochondrial membranes.

  5. 5.

    These findings are discussed in relation to the physiology of the fish.

This is a preview of subscription content, log in to check access.


  1. Basile, C., Goldspink, G., Modigh, M., Tota, B.: Morphological and biochemical characterisation of the inner and outer layers of the ventricular myocardium of adult tuna fish (Thunnus thynnus L.). Comp. Biochem. Physiol. (in press)

  2. Carey, F.G., Teal, J.M.: Regulation of body temperature by the bluefin tuna. Comp. Biochem. Physiol.28, 205–213 (1969)

  3. Carey, F.G., Teal, J.M., Kannisher, J.W., Lawson, K.D., Beckett, J.S.: Warmbodied fish Amer. Zool.11, 137–145 (1971)

  4. Chance, B., Williams, G.R.: The respiratory chain and oxidative phosphorylation. Advanc. Enzymol.17, 65–134 (1956)

  5. Don Stevens, E., Fry, F.E.J.: Brain and muscle temperatures in ocean caught and captive skipjack tuna. Comp. Biochem. Physiol.38A, 203–211 (1971)

  6. Fry, F.E.J.: Responses of vertebrate poikilotherms to temperature. In: Thermobiology (ed. A.H. Rose), p. 375–409. New York: Academic Press 1967

  7. Gornall, A.G., Bardawill, C.J., David, M.A.: Determination of serum proteins by means of the biuret reaction. J. biol. Chem.177, 751–766 (1948)

  8. Hazel, J.R., Prosser, C.L.: Molecular mechanisms of temperature compensation in poikilotherms. Physiol. Rev.54, 620–677 (1974)

  9. Hochachka, P.W., Somero, G.N.: Biochemical adaptation to the environment. In: Fish physiology, vol. VI (W.S. Hoar and J.D. Randall, eds.), p. 100–156. New York: Academic Press 1971

  10. Johnston, I., Tota, B.: Myofibrillar ATPase in white and red myotomal muscles of tuna fish. Comp. Biochem. Physiol.49A 367–373 (1974)

  11. Lehninger, A.L.: The mitochondrion. New York: W.A. Benjamin 1965

  12. Lyons, J.M., Raison, J.K.: A temperature-induced transition in mitochondrial oxidation: Contrasts between cold and warm-blooded animals. Comp. Biochem. Physiol.37, 405–411 (1970)

  13. Paléus, S., Tota, B., Hultin, E., Liljeqvist, G.: The determination of cytochrome c reductase activity in the Keilin and Hartree beef heart preparation. Acta chem. scand.23, 1–6 (1969)

  14. Satchell, G.H.: Circulation in fishes, p. 6–7. Cambridge: Cambridge University Press 1971

  15. Smith, C.L.: The temperature depedence of oxidative phosphorylation and of the activity of the various enzyme systems in liver mitochondria from cold-and warm-blooded animals. Comp. Biochem. Physiol.46B, 445–461 (1973)

  16. Somero, G.N.: Molecular mechanisms of temperature compensation in aquatic poikilotherms. In: Hibernation and hypothermia, perspectives and challenges (F.E. South, J.P. Hannon, J.R. Willis, E.G. Pengelly, and R.N. Alpert, eds.), p. 55–80. New York: Elsevier 1972

  17. Tota, B.: On the respiratory metabolism of the inner and outer layers of tuna fish heart ventricle. Pubbl. Staz. Zool. Napoli38, 223–225 (1970)

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Maresca, B., Modigh, M., Servillo, L. et al. Different temperature dependences of oxidative phosphorylation in the inner and outer layers of tuna heart ventricle. J Comp Physiol B 105, 167–172 (1976). https://doi.org/10.1007/BF00691118

Download citation


  • Oxidation
  • Human Physiology
  • Succinate
  • Mitochondrial Membrane
  • Outer Layer