Purified McAbs (14AC1) of IgG2a isotype raised against an experimental rat glioma (79 FR-G-41) were labeled with Na131I and used for in vivo imaging of glioma grafts by external body seintigraphy. Normal mouse131I-IgG was applied as control for non-specific uptake of proteins in the tumor. Nude mice bearing glioma grafts were injected i.v. with 15 μg of the131I-McAb or131I-IgG with an activity of approximately 150 μCi. Scands obtained 30 min, 24, 48, 72, and 96 h after injecting the intact131I-14AC1 antibody demonstrated enrichment of radioactivity in the tumors. The tumors were clearly visible 48 h after injection of131I-labeled antibody. The time course experiments showed that the uptake of131I-14AC1 antibody in the glioma grafts was the result of specific antigen binding. Intact antibody provided adequate tumor visualization in the scientigrams without background subtraction. Therefore, this technique appears promising for in vivo tumor detection and may offer the possibility of improvement in the evaluation of diagnostic and therapeutic approaches to human gliomas.
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Ballou B, Levine G, Hakala T, Solter D (1979) Tumor location detected with radioactively labeled monoclonal antibody and external scintigraphy. Science 206:844–857
Bourton MA, Coleman RE, Blasberg RG, Groothuis DR, Bigner DD (1984) Monoclonal antibody localization in subcutaneous and intracranial human glioma xenografts: Paired-label and imaging analysis. Anticancer Res 4:133–140
Epenetos AA, Nimmon CC, Arklie J, Elliott AT, Hawkins LA, Knowles RW, Britton KE, Bodmer WF (1982a) Detection of human cancer in an animal model using radiolabelled tumor-associated monoclonal antibodies. Br J Cancer 46:1–8
Epenetos AA, Bodmer WF, Britton KE (1982b) Radioimmunodetection of cancer. Eur J Cancer Clin Oncol 18: 1221–1223
Greenwood FG, Hunter WM, Glover JS (1963) The preparation of131I-labelled human growth hormone of high specific radioactivity. Biochem J 89:114–123
Herlyn D, Powe J, Alavi A, Mattis JA, Herlyn M, Ernst C, Vaum R, Koprowski H (1983) Radioimmunodetection of human tumor xenografts by monoclonal antibodies. Cancer Res 43:2731–2735
Mach JP, Carrel S, Forni M, Ritschard J, Donath A, Alberto P (1980) Tumor localization of radiolabelled antibodies against carcinoembryonic antigen patients with carcinoma N Engl J Med 303:5–10
Phillips J, Anderson T, Sikora K, Watson J (1983) Localization of malignant glioma by a radiolabelled human monoclonal antibody. J Neurol Neurosurg Psychiatry 46:388–392
Pressman D, Korngold L (1953) The in vivo localization of anti-Wagner osteogenic sarcoma antibodies. Cancer 6:619–623
Stavrou D, Süss C, Bilzer T, Kummer U, de Tribolet N (1983) Monoclonal antibodies reactive with glioma cell lines derived from experimental brain tumors. Eur J Cancer Clin Oncol 19:1439–1449
Stavrou D, Mellert W, Bilzer T, Senekowitsch R, Keiditsch E, Mehraein P (1985) Radioimmunodetection of gliomas by administration of radiolabelled monoclonal antibodies. Experimental data. Anticancer Res 5 (in press)
Supported by the Wilhelm Sander Foundation (grant no. Sta-81.007.2)
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Stavrou, D., Mellert, W., Bilzer, T. et al. Localization of experimental glioma grafts by means of iodinated monoclonal antibodies and radionuclide imaging. Acta Neuropathol 66, 340–342 (1985). https://doi.org/10.1007/BF00690968
- Experimental glioma grafts
- Iodinated monoclonal antibodies
- Radionuclide imaging