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Leptomeres in cultured human muscle

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Summary

Leptomeres, the laminated structures consisting of bundles of very fine filaments separated into bands about 260 nm wide by periodic transverse dense lines 20–80 nm wide, were observed frequently in cultured muscle fibers of 8 patients with acid maltase deficiency, 4 with sporadic, adult-onset idiopathic “autophagic” vacuolar myopathy (that is not acid-maltase deficient) and one with abnormal mitochondria, but in only one of >50 other cultures of normal and denervated human muscle. They were also induced abundantly in cultured normal human muscle by exposure to 0.5 mM DNP.

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References

  1. Askanas, V., Engel, W. K.: A new program for investigating adult human skeletal muscle grown aneurally in tissue culture. Neurology (Minneap.)25, 58–67 (1975)

  2. Askanas, V., Engel, W. K.: A technique of fiber selection from human muscle tissue cultures for histochemical-electronmiscroscopic studies. J. Histochem. Cytochem.23, 144–146 (1975a)

  3. Askanas, V., Engel, W. K.: Chick muscle in tissue culture: the ubiquity of viral infection. Acta Neuropath. (Berl.)32, 271–279 (1975)

  4. Askanas, V., Engel, W. K.: Diseased human muscle in tissue culture: a new approach to the pathogenesis of human neuromuscular disorders. In: Pathogenesis of Human Muscular Dystrophies, ed. L. P. Rowland, pp. 856–871. Amsterdam, Excerpta Medica 1977

  5. Askanas, V., Engel, W. K., Britton, D. E., Adornato, B. T., Eiben, D. M.: An unusual mitochondrial abnormality reincarnatedex aegro and inducedde novo in cultured muscle fibers. Neurology (Minneap.)27, 348 (1977a)

  6. Askanas, V., Engel, W. K., DiMauro, S., Brooks, B. R., Mehler, M.: Adult-onset acid maltase deficiency. Morphologic and biochemical abnormalities reproduced in cultured muscle. New Engl. J. Med.294, 573–578 (1976)

  7. Baker, D.: Electrophysiology of muscle spindle. In: Handbook of Sensory Physiology. Vol. 11, pp. 1–190. Springer, Berlin-Heidelberg-New York: 1973

  8. Banker, B. W., Girvin, P. Y.: The ultrastructural features of the mammalian muscle spindle. J. Neuropath. Exp. Neurol.30, 155–195 (1971)

  9. Carrier, H., Tommasi, M., Kopp, Boisson, D.: Proliferation du système tubulaire transversale au cours d'une myopathie tardive et familiale. J. Neurol. Sci.27, 499–512 (1976)

  10. Engel, W. K.: These essentiality of histo- and cytochemical studies of skeletal muscle in the investigation of neuromuscular disease. Neurology (Minneap.)12, 778–794 (1962)

  11. Fardeau, M.: Personal communication, 1976

  12. Gruner, J. E.: La structure fine du fuseau neuromusculaire humain. Rev. Neurol.104, 490–507 (1961)

  13. Gordon, A. R., Tuffey and Vrbova, G.: A possible mechanism determining the pattern of innervation of skeletal muscle fibers. In: Recent Advances in Myology, eds. W. G. Bradley, D. Gardner-Medwin, I. N. Walton, pp. 22–26. Amsterdam: Excerpta Medica, New York: American Elsevier 1975

  14. Hess, A., Pilar, G.: Slow fibers in the extraocular muscle of the cat. J. Physiol. (London)169, 780–801 (1963)

  15. Hikida, R. A., Bock, J. W.: Analysis of fiber types in the pigeon's metapatagialis muscle. Effects of denervation. Tissue Cell8, 259–276 (1976)

  16. Karlsson, U., Anderson-Cedegren, E.: Small leptomeric organelles in intrafusal muscle fibers of the frog as revealed by electronmicroscopy. J. Ultrastruct. Res.23, 417–426 (1968)

  17. Lake, B. D., Wilson, J.: Zebra-body myopathy. Clinical, histochemical and ultrastructural studies. J. Neurol. Sci.24, 437–446 (1975)

  18. Mair, W. G. P., Tome, F. M. S.: The ultrastructure of the adult and developing human myotendinous junction. Acta Neuropath. (Berl.)21, 239–252 (1972)

  19. Miranda, A. G., Godman, G. C.: The effects of cytochalasin D on differentiating muscle culture. Tissue Cell5, 1–22 (1973)

  20. Martinez, A. Y., Hay, S., McNeer, K. W.: Extraocular muscles. Light microscopy and ultrastructural features. Acta Neuropath. (Berl.)34, 237–253 (1976)

  21. Mukano, K.: The fine structure of human extraocular muscles. A “laminated structure” in the muscle fibers. J. Electron Micr.15, 227–236 (1966)

  22. Ovalle, W. K.: Fine structure of rat intrafusal muscle fibers. The equatorial region. J. Cell Biol.52, 382–396 (1972)

  23. Ringel, S. P., Engel, W. K., Bender, A. N., Peters, N. D., Yee, R. D.: Histochemistry and acetylcholine receptors distribution in different types of fibers in monkey extraocular muscles. Neurology (Minneap.) (In press)

  24. Ruska, H., Edwards, G. A.: A new cytoplasmic pattern in striated muscle fibers and its possible relation to growth. Growth21, 77–88 (1957)

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Correspondence to W. King Engel.

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Askanas, V., Engel, W.K. & Bethlem, J. Leptomeres in cultured human muscle. Acta Neuropathol 42, 247–250 (1978). https://doi.org/10.1007/BF00690365

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Key words

  • Leptomeres
  • Cultured human muscle
  • Myopathy