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Sequestration of arginine by polyphosphate in vacuoles of yeast (Saccharomyces cerevisiae)

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Abstract

Isolated and purified vacuoles from yeast protoplasts contain the bulk of the cellular pool of arginine. The arginine is firmly retained in the isolated vacuoles despite of the presence of a permease which mediates arginine diffusion through the vacuolar membrane (Boller et al., 1975). It is shown, mainly by equilibrium dialysis, on vacuolar extracts, that the retention of arginine in the vacuoles is due to binding by polyphosphate. The polyphosphate appears to be located exclusively in the vacuoles. Enzymes hydrolysing polyphosphate are also located in the vacuoles. Isolated vacuoles from arginine grown cells contain about three times as much polyphosphate as vacuoles from ammonium grown cells; the vacuolar pool of arginine is correspondingly greater. Thus there seems to be a close correlation between the storage of arginine and polyphosphate. This confirms the observation that under conditions provoking “polyphosphate overcompensation” (Liss and Langen, 1962) the accumulation of enormous quantities of polyphosphate is associated with that of corresponding quantities of arginine, provided this amino acid is supplied in the medium. Yet, under certain growth conditions the cells are able to store, and to mobilize, both arginine and polyphosphate independently.

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Abbreviations

PP:

polyphosphate

PO:

orthophosphate

PIPES:

piperazine-N,N′-bis-2-ethanesulfonic acid

HEPES:

N-2-hydroxyethyl-piperazine-N′-2-ethanesulfonic acid

Tris:

2-amino-2-hydroxymethyl-1,3-propanediol

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Correspondence to A. Wiemken.

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Dürr, M., Urech, K., Boller, T. et al. Sequestration of arginine by polyphosphate in vacuoles of yeast (Saccharomyces cerevisiae). Arch. Microbiol. 121, 169–175 (1979). https://doi.org/10.1007/BF00689982

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Key words

  • Yeast
  • Vacuoles
  • Compartmentation
  • Polyphosphate
  • Arginine