Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Sequestration of arginine by polyphosphate in vacuoles of yeast (Saccharomyces cerevisiae)

  • 119 Accesses

  • 71 Citations


Isolated and purified vacuoles from yeast protoplasts contain the bulk of the cellular pool of arginine. The arginine is firmly retained in the isolated vacuoles despite of the presence of a permease which mediates arginine diffusion through the vacuolar membrane (Boller et al., 1975). It is shown, mainly by equilibrium dialysis, on vacuolar extracts, that the retention of arginine in the vacuoles is due to binding by polyphosphate. The polyphosphate appears to be located exclusively in the vacuoles. Enzymes hydrolysing polyphosphate are also located in the vacuoles. Isolated vacuoles from arginine grown cells contain about three times as much polyphosphate as vacuoles from ammonium grown cells; the vacuolar pool of arginine is correspondingly greater. Thus there seems to be a close correlation between the storage of arginine and polyphosphate. This confirms the observation that under conditions provoking “polyphosphate overcompensation” (Liss and Langen, 1962) the accumulation of enormous quantities of polyphosphate is associated with that of corresponding quantities of arginine, provided this amino acid is supplied in the medium. Yet, under certain growth conditions the cells are able to store, and to mobilize, both arginine and polyphosphate independently.

This is a preview of subscription content, log in to check access.







piperazine-N,N′-bis-2-ethanesulfonic acid


N-2-hydroxyethyl-piperazine-N′-2-ethanesulfonic acid




  1. Boller, Th., Dürr, M., Wiemken, A.: Characterization of a specific transport system for arginine in isolated yeast vacuoles. Eur. J. Biochem.54, 81–91 (1975)

  2. Dürr, M., Boller, Th., Wiemken, A.: Action of proteinases on the arginine transport of purified vacuoles fromSaccharomyces cerevisiae. Biochem. Biophys. Res. Commun.73, 193–199 (1976)

  3. Huber-Wälchli, V., Wiemken, A.: Differential extraction of soluble pools from the cytosol and the vacuoles of yeast (Candida utilis) using DEAE-dextran. Arch. Microbiol.120, 141–149 (1979)

  4. Indge, K. J.: Polyphosphate of the yeast cell vacuole. J. Gen. Microbiol.51, 447–455 (1968)

  5. Liss, E., Langen, P.: Versuche zur Polyphosphat-Überkompensation in Hefezellen nach Polyphosphatverarmung. Archiv f. Mikrobiol.41, 383–392 (1962)

  6. Lowry, O. H., Rosebrough, N. J., Farr, J. C., Randall, R.: Protein measurement with the Folin phenol reagent. J. Biol. Chem.193, 265–275 (1951)

  7. Ludwig, J. R., Olivier, S. G., McLaughlin, C. S.: The effect of amino acids on growth and phosphate metabolism in a prototrophic yeast strain. Biochem. Biophys. Res. Commun.79, 16–23 (1977)

  8. Ohnishi, T., Gall, R. S., Mayer, M. L.: An improved assay of inorganic phosphate in the presence of extralabile phosphate compounds: Application to the adenosinetriphosphatase assay in the presence of phosphocreatine. Analyt. Biochem.69, 261–267 (1975)

  9. Peterson, G. L.: A simplification of the protein method of Lowry et al. which is more generally applicable. Anal. Biochem.83, 346 (1978)

  10. Ramos, F., Thuriaux, P., Wiame, J. M., Béchet, J.: The participation of ornithine and citrulline in the regulation of arginine metabolism ofSaccharomyces cerevisiae. Eur. J. Biochem.12, 40–47 (1970)

  11. Riemersma, J. C., Alsbach, E. J. J.: Proton translocation during anaerobic energy production inSaccharomyces cerevisiae. Biochim. Biophys. Acta339, 274–284 (1974)

  12. Roush, A. H.: Crystallization of purines in the vacuoles ofCandida utilis. Nature (Lond.)190, 449 (1961)

  13. Schwencke, J., deRobichon-Szulmajster, H.: The transport of Sadenosyl-l-methionine in isolated yeast vacuoles and spheroplasts. Eur. J. Biochem.65, 49–60 (1976)

  14. Seaston, A., Carr, G., Eddy, A. A.: The concentration of glycine by preparation of the yeastSaccharomyces carlsbergiensis depleated of ATP. Effect of proton gradients and uncoupling agents. Biochem. J.154, 669–676 (1976)

  15. Shabalin, A. Y., Vagabov, V. N., Tsiomenko, A. B., Zemlenukhina, O. A., Kulaev, J. S.: Polyphosphate kinase activity in yeast vacuoles. Biochemistry (USSR)42, 1291–1296 (1977)

  16. Svihla, G., Dainko, J. C., Schlenk, F.: Ultraviolet microscopy of purine compounds in the yeast vacuole. J. Bact.83, 399–402 (1963)

  17. Urech, K., Dürr, M., Boller, Th., Wiemken, A., Schwencke, J.: Localization of polyphosphate in vacuoles ofSaccharomyces cerevisiae. Arch. Microbiol.116, 275–278 (1978)

  18. Wiame, J. M.: Accumulation de l'acide phosphorique (phytine, polyphosphate). In: Encylopedia of plant physiology, Vol. IX (W. Ruhland, ed.), pp. 136–146 Berlin-Göttingen-Heidelberg: Springer 1958

  19. Weimberg, R.: Polyphosphate level in nongrowing cells ofSaccharomyces cerevisiae as determined by magnesium ion and the phenomenon of “Überkompensation”. J. Bact.121, 1122–1130 (1975)

  20. Wiemken, A.: Eigenschaften der Hefevakuole. Thesis, ETH Nr. 4340, Swiss Federal Institute Technol. Zürich (1969)

  21. Wiemken, A., Matile, Ph., Moor, H.: Vacuolar dynamics in synchronously budding yeast. Arch. Microbiol.70, 89–103 (1970)

  22. Wiemken, A., Dürr, M.: Characterization of amino acid pools in the vacuolar compartment ofSaccharomyces cerevisiae. Arch. Microbiol.101, 45–57 (1974)

  23. Yemm, E. W., Cocking, E. C.: The determination of amino acids with ninhydrin. Analyst (Lond.)80, 209–213 (1955)

  24. Zacharsky, C. A., Cooper, T. G.: Metabolite compartmentation inSaccharomyces cerevisiae. J. Bact.135, 490–497 (1978)

Download references

Author information

Correspondence to A. Wiemken.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Dürr, M., Urech, K., Boller, T. et al. Sequestration of arginine by polyphosphate in vacuoles of yeast (Saccharomyces cerevisiae). Arch. Microbiol. 121, 169–175 (1979). https://doi.org/10.1007/BF00689982

Download citation

Key words

  • Yeast
  • Vacuoles
  • Compartmentation
  • Polyphosphate
  • Arginine