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Changes in surgar transport and in electrophysiological characteristics of intestinal preparations of temperature-acclimated goldfish (Carassius auratus L.)

Summary

  1. 1.

    Goldfish were acclimated to temperatures of 10°C and 30°C for at least 4 weeks.

  2. 2.

    Fish acclimated to 10°C have longer intestines with larger mucosal folds than fish acclimated to 30°C.

  3. 3.

    When fish acclimated to 10°C are compared with 30°C fish, the electrical resistance of intestinal mucosa, expressed in Ωcm2 of serosal area, from 10°C-acclimated fish is higher. However when related to mucosal area, the resistance in 30°C-acclimated fish is higher.

  4. 4.

    Mucosal glucose application evokes a change in the transepithelial potential difference. Although this effect is smaller in the intestines of 30°C-acclimated fish, a sustained change in potential difference due to the presence of glucose is observed in both cases.

  5. 5.

    Transepithelial sugar transport across in vitro intestinal preparations was measured using 3-oxy-methyl-d-glucose as a substrate. At 30°C the net flux, expressed in mmol·kg wet weight−1·h−1, is larger in intestinal mucosa of 30°C-acclimated fish than in mucosa of 10°C-fish.

  6. 6.

    Ion content was determined in both freshly isolated muscosa and mucosa incubated for 1 h at 20°C. The sodium concentration in intestinal cells from 30°C-acclimated fish was found to be higher.

  7. 7.

    Oxygen consumption by intestinal mucosa was determined at 20°C in the absence and presence of ouabain. The difference in ouabain-sensitive oxygen consumption between 10°C-fish or 30°C-fish is not significant.

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Abbreviations

3-OMG :

3-oxy-methyl-d-glucose

GEP :

glucose evoked potential change across the epithelium

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Groot, J.A., Albus, H., Bakker, R. et al. Changes in surgar transport and in electrophysiological characteristics of intestinal preparations of temperature-acclimated goldfish (Carassius auratus L.). J Comp Physiol B 151, 163–170 (1983). https://doi.org/10.1007/BF00689914

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Keywords

  • Oxygen Consumption
  • Intestinal Mucosa
  • Ouabain
  • Sodium Concentration
  • Intestinal Cell