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Diglyceride-transporting lipoproteins inLocusta

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Summary

The majority of haemolymph proteins obtained from resting mature locusts are soluble in 50% saturated (NH4)2SO4 solution. Chromatographically, these proteins may be resolved on Sepharose 6B into three partially included fractions. The leading high molecular weight fraction, A, is predominant and all the diglyceride in “resting blood” is associated with it. An additional higher molecular weight fraction, A+, is present in the haemolymph of locusts injected with extracts of the glandular lobes of the corpora cardiaca (“elevated blood”), and this fraction carries all the increased diglyceride. Fraction A+ is better resolved on Ultrogel ACA 22. A useful rapid technique for studying the formation of A+ lipoprotein during lipid mobilization has been developed by the use of lipoproteinpolyanion-metal ion complex formation. A+ is soluble after heparin treatment whereas the yellow leading edge of fraction A precipitates. Concomitant with the appearance of A+ there is a marked depletion of a lower molecular weight non lipid-carrying protein fraction, C. The ability to form fraction A+ after injections of glandular lobe extract cannot be demonstrated in fledglings (immature locusts within 2 or 3 days after the imaginal moult) where fraction C is virtually absent. It is suggested that during lipid mobilization, some lipoprotein from fraction A combines with protein from fraction C to form A+. A direct effect of adipokinetic hormone on the process of A+ formation cannot be excluded.

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References

  1. Bligh, E.G., Dyer, W.J.: A rapid method of total lipid extraction and purification. Canad. J. Biochem. Physiol.37, 911–917 (1959)

  2. Burstein, M., Scholnick, H.R.: Lipoprotein-polyanion-metal interactions. Advanc. Lipid Res.11, 67–108 (1973)

  3. Burstein, M., Scholnick, H.R., Morfin, R.: Rapid method for the isolation of lipoproteins from human serum by precipitation with polyanions. J. Lipid Res.11, 583–595 (1970)

  4. Candy, D.J.: Metabolic studies on locust flight muscle using a new perfusion technique. J. Insect Physiol.16, 531–543 (1970)

  5. Chino, H., Gilbert, L.I.: Diglyceride release from insect fat body: a possible means of lipid transport. Science (N.Y.)143, 359–361 (1964)

  6. Chino, H., Gilbert, L.I.: Lipid release and transport in insects. Biochim. biophys. Acta (Amst.)98, 94–110 (1965)

  7. Chino, H., Murakami, S., Harashima, K.: Diglyceride-carrying lipoproteins in insect haemolymph: isolation, purification and properties. Biochim. biophys. Acta (Amst.)176, 1–26 (1969)

  8. Chino, H., Sudo, A., Harashima, K.: Isolation of diglyceride-bound lipoprotein from insect haemolymph. Biochim. biophys. Acta. (Amst.)144, 177–179 (1967)

  9. Cornwell, D.G., Kruger, F.A.: Molecular complexes in the isolation and characterization of plasma lipoproteins. J. Lipid. Res.2, 110–134 (1961)

  10. Goldsworthy, G.J., Mordue, W., Guthkelch, J.: Studies on insect adipokinetic hormones. Gen. comp. Endocr.18, 545–551 (1972)

  11. Jutsum, A.R., Argarwal, H.C., Goldsworthy, G.J.: Starvation and haemolymph lipids inLocusta migratoria (R & F). Acrida4, 47–56 (1975)

  12. Jutsum, A.R., Goldsworthy, G.J.: Some effects of mermithid infection on metabolic reserves and flight inLocusta. Int. J. Parasit.4, 625–630 (1974)

  13. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folinphenol reagent. J. biol. Chem.193, 265–275 (1951)

  14. Mayer, R.J., Candy, D.J.: Changes in the haemolymph lipoproteins during locust flight. Nature (Lond.)215, 987 (1967)

  15. Mayer, R.J., Candy, D.J.: Control of haemolymph lipid concentration during locust flight: An adipokinetic hormone from the corpora cardiaca. J. Insect Physiol.15, 611–620 (1969)

  16. Mordue, W., Goldsworthy, G.J.: Physiological effects of corpus cardiacum extracts in locusts. Gen. comp. Endocr.12, 360–369 (1969)

  17. Mwangi, R.W., Goldsworthy, G.J.: Age-related changes in the response to adipokinetic hormone inLocusta migratoria. Physiol. Entomol., in press (1976a)

  18. Mwangi, R.W., Goldsworthy, G.J.: Age-related changes in the response to adipokinetic hormone inLocusta. Gen. comp. Endocr., in press (1976b)

  19. Peled, Y., Tietz, A.: Fat transport in the locust: the role of protein synthesis. Biochim. biophys. Acta (Amst.)296, 499–509 (1973)

  20. Peled, Y., Tietz, A.: Isolation and properties of a lipoprotein from the haemolymph of the locust,Locusta migratoria. Insect Biochem.5, 61–72 (1975)

  21. Robinson, N.L., Goldsworthy, G.J: Adipokinetic hormone and the regulation of carbohydrate and lipid metabolism in a working flight muscle. J. Insect Physiol, in press (1976a)

  22. Robinson, N.L., Goldsworthy, G.J.: Adipokinetic hormone and locust flight muscle metabolism. Gen. comp. Endocr., in press (1976b)

  23. Siakotos, A.N.: The conjugated plasma proteins of the American cockroach. I. The normal state. J. gen. Physiol.43, 999–1013 (1960a)

  24. Siakotos, A.N.: The conjugated plasma proteins of the American cockroach. II. Changes during moulting and clotting processes. J. gen. Physiol.43, 1015–1030 (1960b)

  25. Thomas, K.K., Gilbert, L.I.:In vitro studies on the release and transport of phospholipids. J. Insect Physiol.13, 963–980 (1967)

  26. Thomas, K.K., Gilbert, L.I.: Isolation and characterization of the haemolymph lipoproteins of the American Silkmoth,Hyalophora cecropia. Arch. Biochem. Biophys.127, 512–521 (1968)

  27. Thomas, K.K., Gilbert, L.I.: The haemolymph lipoproteins of the silkmothHyalophora gloveri: studies on lipid composition, origin and function. Physiol. Chem. Physics1, 293–311 (1969)

  28. Tietz, A.: Fat transport in the locust. J. Lipid Res.3, 421–426 (1962)

  29. Tietz, A.: Fat transport in the locust: the role of diglycerides. Europ. J. Biochem.2, 236–242 (1967)

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Mwangi, R.W., Goldsworthy, G.J. Diglyceride-transporting lipoproteins inLocusta . J Comp Physiol B 114, 177–190 (1977). https://doi.org/10.1007/BF00688968

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Keywords

  • Heparin
  • Protein Fraction
  • Elevated Blood
  • Diglyceride
  • Leading Edge