Acta Neuropathologica

, Volume 35, Issue 1, pp 13–25 | Cite as

In vitro characteristics of a sacrococcygeal chordoma maintained in tissue and organ culture systems

  • Bruce C. Horten
  • Stephen R. Montague
Original Investigations


Explants of a human sacral chordoma were successfully maintained on collagen-coated coverslips, gelfoam sponge matrices, and Millipore filter platforms for up to 30 days. Tumor cells cultured on collagen-coated coverslips became increasingly vacuolated whereas those maintained in organ culture were entirely free of vacuoles after 22 days in vitro. A single basic tumor cell, small and polygonal with a large central spherical nucleus and abundant endoplasmic reticulum and Golgi apparatus, was recognized. Vacuoles were formed as the result of the progressive expansion of smooth endoplasmic reticulum. Coalescence of these vacuoles produced the physaliferous cell of light microscopy.

Key words

Chordoma Tissue and organ culture Electron microscopy 


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  1. Botticelli, A., Pavetto, C., Pozzi, F., Sannicola Botticelli, C.: Sul cordoma sacrococcigeo. Aspetti istomorfologici e istochimici. Arch. De Vecchi Anat. pat.53, 577–596 (1968)Google Scholar
  2. Cancilla, P., Morecki, R., Hurwitt, E. S.: Fine structure of a recurrent chordoma. Arch. Neurol. (Chic.)11, 289–295 (1964)Google Scholar
  3. Capelli, A., Jasonni, V., Pizzoferrato, A.: Profilo morfologico ed istochimico dei cordomi. Arch. ital. Anat. Ist. pat.42, 46–92 (1968)Google Scholar
  4. Cappell, D. F.: Chordoma of the vertebral column with three new cases. J. Path. Bact.31, 797–814 (1928)Google Scholar
  5. Cesarini, J.-P., Bonneau, H.: A propos de l'étude ultrastructurale d'un chordome sacré. Arch. Anat. path.19, 95–102 (1971)Google Scholar
  6. Congdon, C. C.: Proliferative lesions resembling chordoma following puncture of nucleus pulposus in rabbits. J. nat. Cancer Inst.12, 893–907 (1952)Google Scholar
  7. Crawford, T.: The staining reactions of chordoma. J. clin. Path.11, 110–113 (1958)Google Scholar
  8. Dahlin, D. C., MacCarty, C. S.: Chordoma: A study of 59 cases. Cancer5, 1170–1178 (1952)Google Scholar
  9. Erlandson, R. A., Tandler, B., Lieberman, P. H., Higinbotham, N. L.: Ultrastructure of human chordoma. Cancer Res.28, 2115–2125 (1968)Google Scholar
  10. Friedmann, I., Harrison, D. F. N., Bird, E. S.: The fine structure of chordoma with particular reference to the physaliphorous cell. J. clin. Path.15, 116–125 (1962)Google Scholar
  11. Gessaga, E. C., Mair, W. G. P., Grant, D. N.: Ultrastructure of a sacrococcygeal chordoma. Acta neuropath. (Berl.)25, 27–35 (1973)Google Scholar
  12. Harvey, W. F., Dawson, E. K.: Chordoma. Edinb. med. J.48, 713–730 (1941)Google Scholar
  13. Horwitz, T.: Chordal ectopia and its possible relation to chordoma. Arch. Path.31, 354–362 (1941)Google Scholar
  14. Kay, S., Schatzki, P. F.: Ultrastructural observations of a chordoma arising in the clivus. Human Path.3, 403–413 (1972)Google Scholar
  15. Müller, H.: Über das Vorkommen von Resten der Chorda dorsalis bei Menschen nach der Geburt und über ihr Verhältnis zu den Gallertgeschwülsten am Clivus. Z. rat. Med.2, 202–229 (1858)Google Scholar
  16. Murad, T. M., Murthy, M. S. N.: Ultrastructure of a chordoma. Cancer25, 1204–1215 (1970)Google Scholar
  17. Peña, C. E., Horvat, B. L., Fisher, E. R.: The ultrastructure of chordoma. Amer. J. clin. Path.53, 544–551 (1970)Google Scholar
  18. Pinchuk, V. G., Svigun, V. S., Monastyrskaia, B. D.: Electron microscopic characteristics of caudal chordoma. Arkh. Pat.31, 38–42 (1969)Google Scholar
  19. Rawlinson, D. G., Rubinstein, L. J., Herman, M. M.: In vitro characteristics of a myxopapillary ependymoma of the filum terminale maintained in tissue and organ culture systems. Acta neuropath. (Berl.)27, 185–200 (1974)Google Scholar
  20. Ribbert, H.: Über die Ecchondrosis physalifora sphenooccipitalis. Zbl. allg. Path. path. Anat.5, 457–461 (1894)Google Scholar
  21. Ribbert, H.: Über die experimentelle Erzeugung einer Ecchondrosis physalifora. Verh. Kong. inn. Med.13, 455–464 (1895)Google Scholar
  22. Rubinstein, L. J., Herman, M. M.: Studies on the differentiation of human and experimental gliomas in organ culture system. In: Recent results in cancer research, vol. 51 (ed. J. Hekmatpanah). Berlin-Heidelberg-New York: Springer 1975Google Scholar
  23. Rubinstein, L. J., Herman, M. M., Foley, V. L.: In vitro characteristics of human glioblastomas maintained in organ culture systems. Light microscopy observations. Amer. J. Path.71, 61–76 (1973)Google Scholar
  24. Soffer, D., Brucher, J. M., Wechsler, W.: Zur Feinstruktur menschlicher Chordome. Path. europ.5, 420–441 (1970)Google Scholar
  25. Spjut, H. J., Luse, S. A.: Chordoma: An electron microscopic study. Cancer17, 643–656 (1964)Google Scholar
  26. Tripier, M. F., Hassoun, J., Toga, M.: Étude ultrastructurale d'un chordome. J. neurol. Sci.25, 361–370 (1975)Google Scholar
  27. Virchow, R.: Untersuchungen über die Entwicklung des Schädelgrundes im gesunden und krankhaften Zustande und über den Einfluß derselben auf Schädelform, Gesichtsbildung und Gehirnbau. Berlin: G. Reimer 1857Google Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Bruce C. Horten
    • 1
    • 2
  • Stephen R. Montague
    • 1
    • 2
  1. 1.Department of Pathology (Neuropathology)Stanford University School of MedicineStanfordUSA
  2. 2.Euphrat Laboratories for NeurosurgeryStanford University School of MedicineStanfordUSA

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