A short guide is given for the planning of nerve fiber morphometry using computer-assisted methods. A new computer program is introduced which allows, among other factors, correction for myelin sheath shrinkage. If myelin shrinkage is not corrected for, there will be false high values for axon caliber and false low values for sheath thickness. The magnitude of the skew produced by this factor is shown on hand from computer editing.
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Arbuthnott ER, Ballard KJ, Boyd IA, Kalu KU (1980) Quantitative study of the non-circularity of myelinated peripheral nerve fibres in the cat. J Physiol (Lond) 308:99–123
Arbuthnott ER, Boyd IA, Kalu KU (1980) Ultrastructural dimensions of myelinated peripheral nerve fibers in the cat and their relation to conduction velocity. J Physiol (Lond) 308:125–157
Berthold C-H (1978) Morphology of normal peripheral axons. In: Waxman SG (ed) Physiology and pathobiology of axons. Raven Press, New York, pp 3–63
Berthold C-H, Corneliuson O, Rydmark M (1982) Changes in shape and size of cat spinal root myelinated nerve fibers during fixation and Vestopal-W embedding for electron microscopy. J Ultrastruct Res 80:23–41
Dyck PJ, Low PA, Sparks MF, Hexum LA, Karnes JL (1980) Effect of serum hyperosmolality on morphometry of healthy human sural nerve. J Neuropathol Exp Neurol 39:285–295
Friede RL (1986) Relation between myelin sheath thickness, internode geometry, and sheath resistance. Exp Neurol 92: 234–247
Friede RL, Beuche W (1985) A new approach toward analyzing peripheral nerve fiber populations. I. Variance in sheath thickness corresponds to different geometric proportions of the internodes. J Neuropathol Exp Neurol 44:60–72
Hildebrand C, Hahn R (1978) Relation between myelin sheath thickness and axon size in spinal cord white matter of some vertebrate species. J Neurol Sci 38:421–434
Holland GR (1982) The effect of buffer molarity on the size, shape and sheath thickness of peripheral myelinated nerve fibres. J Anat 135:183–190
Karnes J, Robb R, O'Brien PC, Lambert EH, Dyck PJ (1977) Computerized image recognition for morphometry of nerve attribute of shape of sampled transverse sections of myelinated fibers which best estimates their average diameter. J Neurol Sci 34:43–51
Minwegen P, Friede RL (1984) Conduction velocity varies with osmotically induced changes of the area of the axon's profile. Brain Res 297:105–113
Pfeiffer G, Friede RL (1985) A morphometric study of nerve fiber atrophy in rat spinal roots. J Neuropathol Exp Neurol 44:546–558
Sugimura K, Windebank AJ, Natarajan V, Lambert EH, Schmid HHO, Dyck PJ (1980) Interstitial hyperosmolarity may cause axis cylinder shrinkage in streptozotocin diabetic nerve. J Neuropathol Exp Neurol 39:710–721
Dedicated to Prof. F. Seitelberger on the occasion of his seventieth birthday
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Friede, R.L. Computer editing of morphometric data on nerve fibers. An improved computer program. Acta Neuropathol 72, 74–81 (1986). https://doi.org/10.1007/BF00687950
- Nerve fiber morphometry
- Computer program
- Myelin sheath thickness
- Tissue preparation