Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Suppression of acute experimental allergic encephalomyelitis by synthetic serum thymic factor

Clinical, histopathological, and immunohistochemical studies

  • 12 Accesses

  • 16 Citations


Acute experimental allergic encephalomyelitis (EAE) was induced in Hartley guinea pigs and Lewis rats, which were then treated with synthetic serum thymic factor (FTS). When a dose of 30 μg/100 g body weight of FTS was subcutaneously administered to the animals on days — 1 (before inoculation), 4, 9 and 15 intermittently, clinical symptoms of acute EAE were suppressed. Histopathological evaluation showed that the severity of EAE in FTS-treated guinea pigs was less than in unteated guinea pigs. Immunohistochemical examination showed that the numbers of OX6+, W3/25+, W3/13+ and OX19+ cells in FTS-treated rats were less than in untreated rats and that the number of OX8+ cells in FTS-treated rats was greater than in untreated rats. These findings suggest that FTS induced OX8+ cells in inflammatory lesions and suppressed inflammation in acute EAE.

This is a preview of subscription content, log in to check access.


  1. 1.

    Bach JF (1983) Thymulin (FTS-Zn). Clin Immunol Allergy 3:133–156

  2. 2.

    Bach JF, Dardenne M, Pleau JM, Bach MA (1975) Isolation, biochemical characteristics, and biological activity of a circulating thymic hormone in the mouse and in the human. Ann NY Acad Sci 249:186–210

  3. 3.

    Bach JF, Dardenne M, Pleau JM, Rosa J (1977) Biochemical characterisation of a serum thymic factor. Nature 266:55–57

  4. 4.

    Bach JF, Bach MA, Blanot D, Bricas E, Charreire J, Dardenne M, Fournier C, Pleau JM (1978) Thymic serum factor (FTS). Bull Inst Pasteur 76:325–398

  5. 5.

    Bach MA, Niaudet P (1976) Thymic function in NZB mice. II. Regulatory influence of a circulating thymic factor on antibody production against polyvinylpyrrolidone in NZB mice. J Immunol 117:760–764

  6. 6.

    Bach MA, Fournier C, Bach JF (1975) Regulation ofϑ-antigen expression by agents altering cyclic AMP level and by thymic factor. Ann NY Acad Sci 249:316–327

  7. 7.

    Barclay AN (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues. Immunology 42:593–600

  8. 8.

    Ben-Nun A, Cohen IR (1982) Experimental autoimmune encephalomyelitis (EAE) mediated by T cell lines: process of selection of lines and characterization of the cells. J Immunol 129:303–308

  9. 9.

    Bliznakov EG, Wan YP, Chang D, Folkers K (1978) Partial reactivation of impaired immune competence in aged mice by synthetic thymus factors. Biochem Biophys Res Commun 80:631–636

  10. 10.

    Hickey WF, Gonatas NK (1984) Suppressor T-lymphocytes in the spinal cord of Lewis rats recovered from acute experimental allergic encephalomyelitis. Cell Immunol 85:284–288

  11. 11.

    Hickey WF, Gonatas NK, Kimura H, Wilson DB (1983) Identification and quantitation of T lymphocyte subsets found in the spinal cord of the Lewis rat during acute experimental allergic encephalomyelitis. J Immunol 131:2805–2809

  12. 12.

    Lacombe M, Perner F, Dardenne M, Bach JF (1974)Thymectomy in the young pig: effects on the level of circulating thymic hormone. Surgery 76:556–559

  13. 13.

    Lassmann H (1983) Comparative neuropathology of chronic experimental allergic encephalomyelitis and multiple sclerosis. Springer, Berlin Heidelberg New York Tokyo, pp 1–94

  14. 14.

    Lassmann H, Vass K, Brunner Ch, Seitelberger F (1986) Characterization of inflammatory infiltrates in experimental allergic encephalomyelitis. Progr Neuropathol 6:33–62

  15. 15.

    Moore GRW, Traugott U, Stone SH, Raine CS (1985) Dose dependency of MBP-induced demyelination in the guinea pig. J Neurol Sci 70:197–205

  16. 16.

    Moore GRW, McCarron RM, Traugott U, McFarlin DE, Raine CS (1987) Critical threshold for dose of myelin basic protein in murine autoimmune encephalomyelitis. J Neurol Sci 77:173–184

  17. 17.

    Nagai Y, Osanai T, Sakakibara K (1982) Intensive suppression of experimental allergic encephalomyelitis (EAE) by serum thymic factor and therapeutic implication for multiple sclerosis. Jpn J Exp Med 52:213–219

  18. 18.

    Pettinelli CB, McFarlin DE (1981) Adoptive transfer of experimental allergic encephalomyelitis in SJL/J mice after in vitro activation of lymph node cells by myelin basic protein: requirement for Lyt 1+2T lymphocytes. J Immunol 127:1420–1423

  19. 19.

    Raine CS (1985) Experimental allergic encephalomyelitis and experimental allergic neuritis. Handb Clin Neurol 47:429–466

  20. 20.

    Rovenský J, Grimová J, Pekárek J, Korčáková L (1981) The effects of thymosin (fraction V) on adjuvant diseases in rats. Thymus 3:51–53

  21. 21.

    Schulof RS, Goldstein AL (1981) Thymosins and other thymic hormones. In: Hadden J, Stewart M (eds) The lymphokines Humana Press, Clifton, pp 397–423

  22. 22.

    Trotter J, Sriram S, Rassenti L, Chou CHJ, Fritz RB, Steinman L (1985) Characterization of T cell lines and clones from SJL/J and (BALB/cxSJL/J)F1 mice specific for myelin basic protein. J Immunol 134:2322–2327

  23. 23.

    Wiśniewski HM, Keith AB (1977) Chronic relapsing experimental allergic encephalomyelitis: an experimental model of multiple sclerosis. Ann Neurol 1:144–148

  24. 24.

    Woyciechowska J, Goldstein AL, Driscoll B (1985) Experimental allergic encephalomyelitis in guinea pigs: influence of thymosin fraction V on the disease. J Neuroimmunol 7:215–219

Download references

Author information

Correspondence to S. Kato.

Additional information

Supported partly by a grant (1987) from the Neuroimmunological Disorders Research Committee, the Ministry of Health and Welfare of Japan

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kato, S., Nakamura, H. Suppression of acute experimental allergic encephalomyelitis by synthetic serum thymic factor. Acta Neuropathol 75, 337–344 (1988). https://doi.org/10.1007/BF00687786

Download citation

Key words

  • Experimental allergic encephalomyelitis
  • Serum thymic factor
  • Suppressor T cell
  • Immunohistochemistry